[Show abstract][Hide abstract] ABSTRACT: To study the effects of polychlorinated biphenyl (PCB126) on the expression of c-fos and c-jun in mesenchymal cells (MSCs) of rats.
MSCs were separated by percoll cell separating medium (density 1.073 g/cm3). After being cultured and transferred, the cells were divided into a control group, a low-dose group (10(-7) mol/L PCB126) and a high-dose group (10(-6) mol/L PCB126). The cellular growth rate and the expression of c-fos and c-jun protein were analyzed by MTT, immunofluorescence chemical assay, RT-PCR and Western blot.
The cellular growth rate in the low-and high-dose group were 13.8% and 19.1% at 12 h, 31.5% and 36.1% at 24 h, 42.5% and 43.6% at 48 h, respectively (P < 0.01). The positive rates of c-fos expression in the low-and high-dose groups were 54.6% and 51.3% at 12 h, and 83.2% and 73.0% at 24 h. The expression of c-fos mRNA detected by RT-PCR was upregulated at 30 min and 1h in PCB126 groups. The expression of c-jun mRNA were much higher in PCB126 groups than that in the control group. The expression of c-fos and c-jun protein was upregulated in the low-and high-dose groups at 12 h and 24 h.
PCB126 could promote the proliferation of MSCs and upregulate the expression of cancer associated gene c-fos and c-jun. The effect of PCB126 on the function of MSCs might be associated with the abnormal expression of c-fos and c-jun gene.
No preview · Article · Mar 2012 · Wei sheng yan jiu = Journal of hygiene research
[Show abstract][Hide abstract] ABSTRACT: This study sought to elucidate the effect of mechanical strain on the differentiation of mesenchymal stem cells into osteoblasts. Under the conditons of inducing osteoblasts, Immunohistochemical methods and RT-PCR technology were applied in osteogenic supplements medium to detect: (1) the expression of Alkaline phosphatase (ALP), Type I collagen (COL I ), Osterx (Osx) and Osteocalcin (OCN) mRNA, with cyclic strain (3%, 0.5 Hz) applied for 15 min, 30 min, 1 h, 2 h, 4 h, 3 d, 7 d, 14 d; (2) the expression of Osx mRNA and OCN mRNA with 3% strain for 1 h. The results showed: (1) ALP mRNA expression was higher at 7 days; COL I mRNA expression was greater obviously at 7 days and 14 days than that at 3 days and that of the unstrained cells; (2) the expression of Osx mRNA was up-regulated after 15min by strain stimulation,which was significantly increased at 30 min and 1 h in the unstrained cells. The expression of OCN mRNA was not affected in the unstrained cells at 15 min, whereas strain could promote the expression of OCN mRNA at this period. The expression of OCN mRNA was more obviously upregulated in the strained cells at 30 min and 1 h when compared with that in the unstrained cells; (3) the strain (1% and 3%) significantly promoted the expression of Osx mRNA; 10% strain had a little effect on Osx mRNA expression. The expression of OCN mRNA was up-regulated by 3% strain, whereas it had little effect at 1% and 10% strain. In summary, mechanical strain can promote the differentiation of mesenchymal stem cells into osteoblasts.
No preview · Article · Jul 2009 · Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi