[Show abstract][Hide abstract] ABSTRACT: Culture conditions were optimized for somatic embryogenesis ofPanax ginseng. The highest frequency of embryo formation was obtained when tissues were excised from the middle region of the cotyledon
segments of zygotic embryos. Only treatment with light could stimulate the formation of single-type somatic embryos, whereas
multiple-type somatic embryos and calli were observed under dark conditions. The highest production of somatic embryos was
found with an NH4
+:NO3 ratio of 21:39. Among the tested media (MS, B5, and SH), maximum formation of somatic embryos was obtained when cotyledon
expiants were cultured on an 1% agar MS medium supplemented with 5% sucrose. Regenerated ginseng plantlets were transferred
to an autoclaved soil mixture in the greenhouse. These transformants showed no detectable variations in their morphology or
growth characteristics compared with the donor plant.
Full-text · Article · Oct 2006 · Journal of Plant Biology
[Show abstract][Hide abstract] ABSTRACT: Hypocotyl expiants from 22 cultivars ofCatharanthus roseus were cultured on various shoot-inducing media to assess their competence for adventitious shoot formation. The Murashige
and Skoog (MS) media had been supplemented with 14 μM zeatin and 2.5 μM indole-3-butyric acid (IBA), 4.5 μM BA and 0.5 μM
α-naphthaleneacetic acid (NAA), or 14 μM thidiazuron and 2.5 μM IBA. After eight weeks, the expiants from ‘Cooler Raspberry
Red’ showed the greatest frequency of adventitious shoot formation, followed by ‘Cooler Orchid’ and ‘Cooler Treated’. The
highest frequency (86.7%) for ‘Cooler Raspberry Red’ was attained on the medium enhanced with 14 μM zeatin and 2.5 μM NAA.
Excised adventitious shoots were then readily rooted on a half-strength MS basal medium. Afterward, the regenerated plantlets
were transferred to potting soil and grown to maturity in a greenhouse.
Full-text · Article · May 2003 · Journal of Plant Biology