Daniel Schellhorn

University of Nebraska at Lincoln, Lincoln, Nebraska, United States

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Publications (1)3.02 Total impact

  • Suvajit Sen · Jiong Yu · Mamoru Yamanishi · Daniel Schellhorn · Ruma Banerjee
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    ABSTRACT: Cystathionine beta-synthase plays a key role in the intracellular disposal of homocysteine and is the single most common locus of mutations associated with homocystinuria. Elevated levels of homocysteine are correlated with heart disease, Alzheimer's and Parkinson's diseases, and neural tube defects. Cystathionine beta-synthase is modular and subjected to complex regulation, but insights into the structural basis of this regulation are lacking. We have employed hydrogen exchange mass spectrometry to map peptides whose motions are correlated with transmission of intrasteric inhibition and allosteric activation. The mass spectrometric data provide an excellent correlation between kinetically and conformationally distinguishable states of the enzyme. We also demonstrate that a pathogenic regulatory domain mutant, D444N, is conformationally locked in one of two states sampled by the wild type enzyme. Our hydrogen exchange data identify surfaces that are potentially involved in the juxtaposition of the regulatory and catalytic domains and form the basis of a docked structural model for the full-length enzyme.
    No preview · Article · Dec 2005 · Biochemistry

Publication Stats

15 Citations
3.02 Total Impact Points

Top Journals


  • 2005
    • University of Nebraska at Lincoln
      • Department of Biochemistry
      Lincoln, Nebraska, United States