Chao-qun Xu

Chongqing Municipal Academy of Chinese Materia Medica, Ch’ung-ch’ing-shih, Chongqing Shi, China

Are you Chao-qun Xu?

Claim your profile

Publications (1)0 Total impact

  • Qin He · Zhi-rong Zhang · Ji Liu · Chao-qun Xu
    [Show abstract] [Hide abstract]
    ABSTRACT: To prepare TK-gene nanoparticles and investigate its expression. Biodegradable and biocompatible polymer polylactic-co-glycolic acid (PLGA) was used to prepare recombinant plasmid pEGFP-AFP nanoparticles by double-emulsion evaporation technique. The characteristics of the nanopticicles including morphology, entrapment efficiency was investigated. The expression of TK gene was also investigated by MTT assay, which could determine the dying cells after the addition of gancyclovir (GCV). The enhanced green fluorescent protein (EGFP) expression in human hepatocarcinoma SMMC-7221 cells and human normal parenchymal Chang liver cells were assessed by flow cytometric analysis. The resulting plasmid-nanoparticles had regular spherical surface and a narrow particle size with a mean diameter of (72 +/- 12) nm, The average entrapment efficiency was 91.25%, the enhanced transfection efficiency and ability protecting plasmid DNA from degraded by nuclease or sonication due to nanoparticles encapsulation. DNA-nanoparticles need further study as gene delivery system.
    No preview · Article · May 2004 · Yao xue xue bao = Acta pharmaceutica Sinica

Top co-authors


  • 2004
    • Chongqing Municipal Academy of Chinese Materia Medica
      Ch’ung-ch’ing-shih, Chongqing Shi, China