Arife Alev Karagözler

Adnan Menderes University, Güsel Hissar, Aydın, Turkey

Are you Arife Alev Karagözler?

Claim your profile

Publications (11)22.98 Total impact

  • Murat Uygun · A Alev Karagözler · Adil Denizli
    [Show abstract] [Hide abstract]
    ABSTRACT: Molecularly imprinted PHEMAH cryogels were synthesized and used for purification of carbonic anhydrase from bovine erythrocyte. Cryogels were prepared with free radical cryopolymerization of 2-hydroxyethyl methacrylate and methacryloylamido histidine and characterized by swelling degree, macroporosity, FTIR, SEM, surface area and elemental analysis. Maximum carbonic anhydrase adsorption of molecularly imprinted PHEMAH cryogel was found to be 3.16 mg/g. Selectivity of the molecularly imprinted cryogel was investigated using albumin, hemoglobin, IgG, γ-globulin, and lysozyme as competitor proteins and selectivity ratios were found to be 15.26, 60.05, 21.88, 17.61, and 17.42, respectively. Carbonic anhydrase purity was demonstrated by SDS-PAGE and zymogram results.
    No preview · Article · Feb 2014 · Artificial Cells
  • M.A. Demiral · D. Aktaş Uygun · M. Uygun · E. Kasirǧa · A.A. Karagözler
    [Show abstract] [Hide abstract]
    ABSTRACT: One-year-old olive (Olea europaea L. cv. Gemlik) seedlings were exposed to increasing levels of salinity in pot culture. The experiment was laid out in a randomized block design with 6 replicates. Dry matter (DM) percentage, sodium (Na) and chloride (Cl) concentrations, 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging activity, reducing power (RP), total phenolic content (TPC), and proline (PRO) content were determined in the leaves of the plants. The results showed that the olive plant used physiological and biochemical mechanisms consecutively to alleviate the effects of salt stress. The type of mechanism used was related to the severity of the salt stress. Salinity-induced variation in DPPH scavenging activity correlated well with the PRO and TPC variations of the leaves. The results showed that DPPH radical scavenging activity may be evaluated as a reliable parameter to assess the ability of antioxidants to hinder salt stress in olive plants.
    No preview · Article · Jan 2011
  • Deniz Aktas Uygun · A. Alev Karagözler · Sinan Akgöl · Adil Denizli
    [Show abstract] [Hide abstract]
    ABSTRACT: N-Methacryloyl-l-phenylalanine (MAPA) containing poly(2-hydroxyethylmethacrylate) based magnetic [mag-poly(HEMA–MAPA)] nanobeads was prepared for lysozyme purification form chicken egg white. MAPA was synthesized by reacting methacryloyl chloride with l-phenylalanine methyl ester and provided hydrophobic functionality to the nanobeads. Size of mag-poly(HEMA–MAPA) nanobeads was 386 nm and obtained by surfactant free emulsion polymerization of HEMA and MAPA having a specific surface area of 580 m2/g. Mag-poly(HEMA–MAPA) nanobeads were characterized by FTIR, AFM, TEM, ESR, and elemental analysis. Lysozyme adsorption experiments were investigated under different conditions in batch system (i.e., medium pH, protein concentration, temperature, salt type). Lysozyme adsorption capacity of mag-poly(HEMA) and mag-poly(HEMA–MAPA) nanobeads from aqueous solutions was estimated as 24 and 517 mg/g, respectively. Lysozyme molecules were desorbed with 50% ethylene glycol solution with 98% recovery. It was observed that mag-poly(HEMA–MAPA) nanobeads can be used without significant decrease in lysozyme adsorption capacity after ten adsorption–desorption cycles. Mag-poly(HEMA–MAPA) nanobeads was used for the purification of lysozyme from chicken egg white. Purity of lysozyme was estimated by SDS-PAGE.
    No preview · Article · Aug 2009 · Materials Science and Engineering C
  • [Show abstract] [Hide abstract]
    ABSTRACT: Linoleic acid attached chitosan beads [poly(LA-Ch)] (1.25 microm in diameter) are obtained by the formation of amide linkages between linoleic acid and chitosan. Poly(LA-Ch) beads are characterized by FTIR, TEM, and swelling studies. Poly(LA-Ch) beads are used for the purification of immunoglobulin-G (IgG) from human plasma in a batch system. The maximum IgG adsorption is observed at pH 7.0 for HEPES buffer. IgG adsorption onto the plain chitosan beads is found to be negligible. Adsorption values up to 136.7 mg/g from aqueous solutions are obtained by poly(LA-Ch) beads. IgG adsorption saw an increase as a result of increasing temperature. Higher amounts of IgG are adsorbed from human plasma (up to 390 mg/g) with a purity of 92%. The adsorption phenomena appeared to follow a typical Langmuir isotherm. It is observed that IgG could be repeatedly adsorbed and desorbed without significant loss when we take into account the adsorption amount. It is concluded that the poly(LA-Ch) beads allowed one-step purification of IgG from human plasma.
    No preview · Article · Feb 2009 · Colloids and surfaces B: Biointerfaces
  • Source
    Arife Alev Karagözler · Bengi Erdağ · Yelda Çalmaz Emek · Deniz Aktaş Uygun
    [Show abstract] [Hide abstract]
    ABSTRACT: Dorystoechas hastata (D. hastata) is a monotypic plant endemic to Antalya province of Turkey. D. hastata leaves are used to make a tea locally called "çalba tea". Diethyl ether (E), ethanol (A), and water (W) were used for the sequential preparation of extracts from dried D. hastata leaves. A hot water extract (S) was also prepared by directly boiling the powdered plant in water. The antioxidant activities of the extracts were tested by ferric thiocyanate (FTC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging methods. E extract exhibited the greatest antioxidant activity with FTC method, whereas S extract exhibited the lowest IC50 value (6.17±0.53μg/ml) for DPPH radical scavenging activity. Total phenolic contents of the extracts were estimated by Folin-Ciocalteu method and S extract was found to contain the highest amount (554.17±20.83mg GAE/g extract) of phenolics. Extract A contained highest flavonoid content and there was a inverse linear correlation (R(2)=0.926) between IC50 values for DPPH radical scavenging activity and flavonoid contents of all extracts. Reducing power of extracts increased in a concentration-dependent manner. S extract was found to possess higher reducing power than equivalent amount of ascorbic acid at 20 and 25μg/ml concentrations. Linear correlation between reducing power and concentration of E, A, and W extracts (R(2)>0.95) was observed. A, W, and S extracts contained relatively high levels of proline. The results presented suggest that D. hastata may provide a natural source of antioxidants and proline. Copyright © 2008 Elsevier Ltd. All rights reserved.
    Full-text · Article · Nov 2008 · Food Chemistry
  • [Show abstract] [Hide abstract]
    ABSTRACT: Poly(ethylene glycol dimethacrylate-n-vinyl imidazole) [poly(EGDMA-VIM)] beads (average diameter 150–200 μm) was prepared by copolymerizing ethylene glycol dimethacrylate (EGDMA) with n-vinyl imidazole (VIM). Average pore size of poly(EGDMA-VIM) beads was 550 nm. The copolymer beads composition was characterized by elemental analysis and found to contain five EGDMA monomer units each VIM monomer unit. Poly(EGDMA-VIM) beads had a specific surface area of 59.8 m2/g. Poly-(EGDMA-VIM) beads were characterized by swelling studies and SEM. Cu2+ ions were chelated on the poly(EGDMA-VIM) beads, then these beads were used in the adsorption of uricase from Porcine Liver in batch system. The maximum uricase adsorption capacity of the poly(EGDMA-VIM)-Cu2+ beads was observed as 118.3 mg/g at pH 6.0. The Km values for immobilized uricase (poly(EGDMA-VIM)-Cu2+) (91.95 × 10−3 mM) was higher than that of free enzyme (7.5 × 10−3 mM). Vmax was calculated as 0.012 μmol/min mg protein for the free enzyme. For the immobilized enzyme, Vmax was calculated as 1.44 μmol/min mg protein. Free enzyme lose all of original activity in 35 days. On the other hand immobilized enzyme preserved 80% of original activity in same time. Storage stability was found to increase with immobilization. It was observed that enzyme could be repeatedly adsorbed and desorbed without significant loss in adsorption capacity or enzyme activity.
    No preview · Article · Mar 2008 · Journal of Molecular Catalysis B Enzymatic
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to examine occupation-associated effects on blood cytokine and immunoglobulin g (IgG) levels, antioxidant enzyme activities (SOD, GSHPx, and catalase), nitrite and nitrate levels, and malondialdehyde (MDA) concentrations in gas station workers (GSWs). Trace elements (Cu, Zn, and Pb) in serum, and the amount of hemoglobin in erythrocytes were also determined. Forty male GSWs and 30 male controls were included in the study. No significant difference was observed between the hemoglobin levels of GSWs and those in controls. Proinflammatory cytokine levels, such as tumor necrosis factor-α and interleukin-8 were significantly increased in GSWs’ sera. Interleukin-1β and interleukin-6 were below the detection limit of the assay in both the groups. Cytokine receptor interleukin 2R concentration and IgG levels were also significantly increased. Approximately, two-fold increase in nitrite and nitrate concentrations was found in GSWs’ sera. Erythrocyte antioxidant enzyme levels were significantly lower in GSWs’ blood. MDA concentration, a measure of lipid peroxidation, was found to be significantly elevated. Zn levels were significantly decreased, whereas Cu and Pb levels were increased in GSWs’ sera. Smoking was not found to be a major confounder for the association between exposure and blood parameters.
    No preview · Article · Oct 2006 · Toxicological and Environmental Chemistry
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this work, the protective effect of Vitamin E plus selenium (Vit E+Se) and melatonin against 7,12-dimethylbenz(a)anthracene (7,12-DMBA)-induced changes in superoxide dismutase (SOD), glutathione peroxidase (GSHPx), catalase (CAT) and carbonic anhydrase (CA) activities and malonedialdehyde (MDA) levels of mouse brain were compared. 12-month old mice were divided into four groups each including 10 animals. The first group served as control group. The second group was treated with 7,12-DMBA (20 mg/(kg day)). The third group was treated with 7,12-DMBA and Vitamin E (90 microg/(individual day)) and selenium (1.8 microg/(individual day)) simultaneously. The fourth group was treated with 7,12-DMBA and melatonin (4.2 mg/(kg day)) simultaneously. Treatment continued for 21 days after which the mice were sacrificed and brain homogenates were prepared. 7,12-DMBA treated group exhibited significantly decreased levels of brain SOD, GSHPx, CAT and CA activities and increased MDA levels as compared to control. Vitamin E+Se fully or partially restored enzyme inhibition except for SOD. Lipid peroxidation was also reduced in Vitamin E+Se treated group. Melatonin provided a better protection for SOD, GSHPx and CAT, and a plausible protection for CA activity. Protection against lipid peroxidation measured as MDA in melatonin treated group was appreciable although slightly lesser than the protection provided by Vitamin E+Se. The results imply that Vitamin E+Se and melatonin both provide chemoprevention against 7,12-DMBA-induced oxidative stress in mouse brain.
    No preview · Article · Feb 2005 · Cancer Detection and Prevention
  • A Alev Karagözler · Nihayet Mehmet · Kadir Batçioglu
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to examine solvent-associated effects on blood cytokine levels, antioxidant enzyme activities, and malondialdehyde (MDA) concentration in house painters. Trace element (Cu and Zn) and nitrite and nitrate levels as well as protein concentrations in erythrocytes and serum were determined. Thirty male house painters and 30 male clerical workers were included in the study. There were 13 smokers and 17 nonsmokers in each group. Hemoglobin concentrations were significantly lower in house-painter blood compared to controls. House painters had significantly higher concentrations of erythrocyte protein (excluding hemoglobin), whereas no significant difference was observed between serum protein levels. Proinflammatory cytokine levels, such as tumor necrosis factor-alpha and interleukin-8, were significantly increased in house painters' sera. Interleukin-6 was below the detection limit of the assay in both groups. Interleukin-1beta and cytokine receptor interleukin 2R concentrations were not significantly affected. Furthermore, a three- to fourfold increase in nitrite and nitrate concentrations was found in house painters' sera. Serum superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) activities were significantly lower in house painters compared to controls. Malondialdehyde (MDA) concentration, a measure of lipid peroxidation, was found to be significantly elevated. In house painters, erythrocyte carbonic anhydrase and catalase activities were elevated approximately 11- fold and 2-fold, respectively. Zinc levels were significantly decreased in house painters' sera. Smoking was not found to be a major confounder for the association between solvent exposure and blood parameters.
    No preview · Article · Oct 2002 · Journal of Toxicology and Environmental Health Part A
  • K Batcioğlu · A A Karagözler · M Genç · S Celik
    [Show abstract] [Hide abstract]
    ABSTRACT: Chemoprevention is a rapidly growing area of oncology that is identifying agents with a potentially preventive role in cancer. In this study, it was our goal to compare the chemopreventive effects of vitamin E plus selenium, and melatonin. Forty female mice were divided into four equal groups. The first group served as control. The second group had i.p. injections of 7,12-dimethylbenz[a]anthracene (DMBA) (20 mg/kg body weight) in corn oil for 21 days. The third group had the same procedure of DMBA injections as the second group and received vitamin E + selenium (90 microg + 1.8 microg/day), simultaneously. The fourth group had DMBA injections and melatonin (4.2 mg/kg body weight), simultaneously. DMBA alone caused significant inhibition of hepatic glutathione peroxidase (GSHPx), catalase (CAT), and superoxide dismutase (SOD) in the second group. In the third group, vitamin E + selenium restored DMBA-induced GSHPx inhibition significantly whereas CAT and SOD inhibition remained essentially unchanged. In the fourth group, melatonin not only significantly decreased DMBA-induced GSHPx inhibition but also fully reversed CAT and SOD inhibitions caused by DMBA. We speculate that melatonin alone provides better chemoprevention against DMBA-induced oxidative stress than the vitamin E+selenium combination.
    No preview · Article · Mar 2002 · European Journal of Cancer Prevention
  • [Show abstract] [Hide abstract]
    ABSTRACT: Timolol as a -adrenoceptor antagonist is a potent antihypertensive and antianginal drug, which was also proved to be effective for the secondary prevention of myocardial infarction. The analysis of timolol, as with other oxprenolols, in ophthalmic solutions and biological liquids using sensitive instrumental methods has gained importance. Investigation of the electrochemical behavior of timolol maleate on a mercury drop electrode reveals the presence of a specific reduction peak of analytical significance. Analysis conditions affecting the properties of this peak were optimized by a systematic study. The limit of detection of about 2.5 ppb, obtained for the DPV technique using SMDE, is comparable with the most sensitive techniques. This method is suitable for routine analysis in that it is simpler and requires no preconcentration step for the analysis of therapeutic doses of the drug.
    No preview · Article · Oct 2001 · Journal of Analytical Chemistry