Ahmad Molaei Rad

Tehran University of Medical Sciences, Teheran, Tehrān, Iran

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Publications (4)6.72 Total impact

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    ABSTRACT: Unlabelled: TARGET AND PURPOSE: Cancer and heart disease are hard maladies in human communities. To recognize these kinds of diseases in primary states can help for remission and decreasing the expenses. One of the best techniques for recognizing is imaging of the tissue. Methods: The main reason of this study is to survey the design of molecules Gd3+ -Defrasirox-DG as a type of glucose labeled with gadolinium to capture more specific cancer tissue and heart by MRI instrument as a technique extremely accurate and sensitive not too costly and lack of radioactive half-life compared with radioactive 18FDG as competing compound. Result: In this research, glucose is combined with Deferasirox for making complexes with gadolinium. With replacing the new compound of advanced imaging technology, it transferred from nuclear medicine to Radiology and the results were evaluated in vitro and in vivo that indicated the success in imaging of the heart and cancer in animal tumor model. Conclusion: The mechanism of cancer cells death is through activation of TNF-α system. At present, due to the lack of radiation and radioactive half-life and low production cost and high access to MRI compared with PET, this compound can be considered as 18FDG opponent in the near future as the new MRI successor agent.
    No preview · Article · May 2013
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    ABSTRACT: Direct electron transfer of horseradish peroxidase, immobilized on a functional membrane-modified gold electrode, was studied. The electrode showed a quasi-reversible electrochemical redox behavior with a formal potential of 60mV (versus Ag/AgCl) in 20mM potassium phosphate buffer solution at pH 7.0 and temperature 25°C. The cathodic transfer coefficient was 0.42 and electron transfer rate constant was evaluated to be 1.6s−1. Furthermore, the modified electrode was used as a biosensor and exhibited a satisfactory stability and sensitivity to H2O2. The linear range of this biosensor for H2O2 determination was from 5.0×10−6 to 1.5×10−4M while its detection limit, based on a signal-to-noise ratio of 3, was 1.3×10−6M. The apparent Michaelis–Menten constant (Kmapp) for immobilized HRP was calculated to be 1.6×10−4M.
    Full-text · Article · Jun 2007 · Electrochimica Acta
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    ABSTRACT: The hydrogen donor couples pyrocatechol-aniline and phenol-aminoantipyrine in the presence of hydrogen peroxide were compared as chromogens for horseradish peroxidase (HRP) assay. UV-Visible spectroscopy and high-performance liquid chromatography analysis indicated that during the HRP biocatalytic process, pyrocatechol-aniline was converted to a pink-colored reagent with a lambda(max) of 510 nm, which was used in the assay of HRP activity. Electrochemical studies revealed adequate electron transfer ability for this color reagent to serve as a proper mediator for HRP also. Using pyrocatechol-aniline a higher sensitivity and lower detection limit was obtained relative to those of the phenol-aminoantipyrine couple, which is commonly used for HRP assay. A relative standard deviation of 2.9% was obtained for 20 HRP activity measurements, indicating a satisfactory reproducibility for this method. In addition, kinetic parameters of K(m) (12.5mM) and V(max) (12.2 mM min(-1)mg(-1)) were calculated for pyrocatechol-aniline. Regarding the superiority of pyrocatechol-aniline, this couple is suggested to be a better hydrogen donor for the HRP spectrophotometric assay.
    Full-text · Article · Apr 2007 · Analytical Biochemistry
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    Soraya Mohammadi · Ahmad Molaei Rad · Morteza Khabiri
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    ABSTRACT: Bacteriorhodopsin (bR) is a transmembrane protein which can exist in a variety form of chemical states. By choosing two states, O state for binary zero and Q state as binary one, it is possible to use this protein as a memory device. In order to increase O state life time and follow that Q state the new matrix was design to make a bR film. Thus, different ratios of Gelatin-polyvinyl alcohol matrices weight/volume were provided in order to find the optimal condition to light absorption. In addition, triethanolamine (TEA) was also used as photosensitizing chemical additive to film forming suspension. It found that the bR film matrix of 0.0005% (w/v) is the best ratio for bR light absorption. Furthermore, TEA changed both absorption and the lifetime of M intermediate. After wards, bR film was chemically immobilized on glassy surface and then illuminated by laser beam in different times. Radiation of bR films in various intervals after chemical immobilization demonstrated that bR ground state; M, P, and Q intermediates of bR photocycle are existed.
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Publication Stats

72 Citations
6.72 Total Impact Points


  • 2013
    • Tehran University of Medical Sciences
      • Department of Medicinal Chemistry
      Teheran, Tehrān, Iran
  • 2007
    • University of Tehran
      • Institute of Biochemistry and Biophysics
      Teheran, Tehrān, Iran