[Show abstract][Hide abstract]ABSTRACT: To improve the preparation of lenticules from human cornea and to obtain their preservation without loss of viable keratocytes.
The epithelium was manually removed after bathing the surface of the cornea with a solution of trypsin and EDTA. Lenticules were prepared by microkeratome resection and viable keratocytes were visualized by staining with thiazolyl blue (MTT).
The pretreatment with trypsin-EDTA allowed the removal of the epithelium without damage to the keratocytes and the stroma. When these lenticules were incubated in Optisol-GS for 7 days at 4 degrees C, they showed a limited thickness increase and a preservation of keratocyte viability.
This procedure allows the preparation of lenticules with viable keratocytes that can be preserved in the cold for at least 1 week.