[Show abstract][Hide abstract] ABSTRACT: The differences in composition and concentrations of volatile allelochemicals between transgenic Bacillus thuringiensis (Bt) cotton and non Bt cotton leaves were compared and analyzed by using gas chromatography-mass spectrometry (GC-MS). The dominant compounds present in both non Bt and Bt cotton leaves were α-caryophyllene (4.62, 1.42), β-myrcene (3.67, 3.68), β-carene (0.31, 0.80), benzene acetaldehyde (0.88, 0.48), heptanoic acid (2.0, 0.67), 1-methoxy, 2-methyl benzene (0.67, 0.30) and acetic acid, phenyl methyl ester (4.32, 2.48). The differences in main components and ratios of volatile allelochemics between Bt cotton and non Bt cotton suggested that, the expression of Bt toxin protein in cotton was found to have an impact on their volatile profiles. Key words: Bt cotton (RCH 2 Bt), non-Bt cotton, gas chromatography-mass spectrometry (GC-MS). ....
Full-text · Article · Oct 2013 · African journal of agricultural research
[Show abstract][Hide abstract] ABSTRACT: Transgenic insecticidal plants based on Bacillus thuringiensis (Bt) endotoxins and transgenic herbicide tolerant plants are widely used in modern agriculture for control of variety of insect pests in fields. Little is known about toxicity of Cry1Ac toxin of Bt cotton. This study presents the cytotoxic and genotoxic effect of crude Cry1Ac toxin extract on human lymphocytes using MTT test, cytokinesis blocked micronucleus assay and erythrolysis tests as parameters. The exposure of human lymphocytes with increasing doses of toxin showed decreased cell survivability (47.08 %) at 72 h incubation. The number of micronuclei formed was found to be 2.52 %. The Cry1Ac toxin has haemolytic action on human lymphocytes. It was concluded that the crude Cry1Ac toxin has possible cytotoxic potential on human lymphocytes with increased dosage of toxin and time of exposure.
[Show abstract][Hide abstract] ABSTRACT: Drought stress triggered the accumulation of malondialdehyde (MDA) and hydrogen peroxide (H2O2) both in non-Bt and Bt cotton with simultaneous production of antioxidant enzymes. And there was no significant difference
between non-Bt and Bt cotton under drought stress. In contrast to this, we observed a significant reduction of Bt toxin proteins
under 72 h of drought stress in Bt cotton.
Additional key wordsantioxidant enzymes–hydrogen peroxide–insecticidal Bt toxin proteins–ion leakage–malondialdehyde
[Show abstract][Hide abstract] ABSTRACT: Nyctanthes arbortristis Linn. is extensively used in Indian traditional and folklore medicines to cure various human ailments. The preliminary phytochemical screening of the leaves revealed the presence of alkaloids, tannins, flavonoids, cardiac glycosides and sugars. In vitro antibacterial studies on the ethanolic leaf extracts were carried out on ten medically important bacterial strains, including Salmonella typimurium, Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli, Psudomonas sp. Staphylococcus epidermis, Micrococcus luteus, Staphylococcus aureus, Streptococccus sp. and Bacillus subtilis, which were procured from the Microbial Type Cultrue and Collection, Chandigarh, India, using agar disc diffusion method. The bacterial strains were exposed to the following four different concentrations of extracts: 50mg/ml, 100mg/ml, 200mg/ml and 300mg/ml solvent. The results of our antibacterial assay revealed that the extract showed good inhibitory activity against all the tested pathogens compared with standard antibiotics like streptomycin and penicillin. The inhibitory activity was found to be dose dependent.