Tomoya Miyazaki

Saga University, Сага Япония, Saga, Japan

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Publications (4)2.84 Total impact

  • [Show abstract] [Hide abstract] ABSTRACT: Integration of previously developed Allium cepa linkage maps requires the availability of anchor markers for each of the eight chromosomes of shallot (A. cepa L. common group Aggregatum). To this end, eight RAPD markers originating from our previous research were converted into SCAR markers via cloning and sequencing of RAPD amplicons and designing of 24-mer oligonucleotide primers. Of the eight pairs of SCAR primers, seven resulted in the amplification of single bands of the original RAPDs, and the remaining primer set amplified an additional band. The results of Southern hybridization using RAPD amplicons from genomic DNA of Japanese bunching onion (Allium fistulosum L.)—shallot monosomic addition lines indicated that five SCAR markers were single shallot chromosome-specific markers and were not detected in genomic DNA of A. fistulosum. The eight SCAR primer pairs were applied to other Allium species and exhibited three types of amplification profiles, namely RAPD amplicons observed only in shallot, in shallot and Allium vavilovii, and in several Allium species. A mapping study using 65 F2 plants generated by the selfing of one interspecific cross A. cepa × Allium roylei individual integrated the SCAR marker SAOE17500 into chromosome 5 as expected. The results of the present study show that the eight SCAR primer sets specific to shallot can facilitate the mapping in A. cepa and can also serve as anchor points between maps of different Allium species.
    No preview · Article · Jan 2007 · Scientia Horticulturae
  • Masayoshi Shigyo · Tomoya Miyazaki · Yosuke Tashiro
    [Show abstract] [Hide abstract] ABSTRACT: Randomly amplified polymorphic DNA (RAPD) analysis was carried out to develop sufficient numbers of PCR-based genetic markers in two cultivated and four wild species of sections Cepa and Phyllodolon in Allium. Sixty random decamer primers were examined, generating large numbers of RAPD bands between the species. A total of 393 RAPDs were detected between the cultivated species, A. fistulosum and shallot (A. cepa var. ascalonicum). The number of RAPDs between A. fistulosum and the four wild species, A. altaicum, A. galanthum, A. oscaninii, A. vavilovii, was respectively 167, 269, 363, 365 and those between shallot and the wild species was respectively 454, 398, 390, 156. These RAPDs will be useful as genetic markers in the two sections. Some of the RAPDs were effective for identifying interspecific hybrids. The RAPD markers successfully confirmed the hybrid nature in ten kinds of F1 plants. Research was also done on the origin of A. wakegi which is well known as a natural hybrid between A. fistulosum and shallot. In 27 clones, species-specific RAPD markers proved the hybrid origin of this plant and that its parental plants are the above mentioned species. Further, the RAPD variations observed in shallot and A. wakegi indicate a polyphyletic generation of initial clones in this species.
    No preview · Article · Jan 2002 · Journal of Horticultural Science and Biotechnology
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    [Show abstract] [Hide abstract] ABSTRACT: Phylogenetic relationships among two cultivated species, Allium fistulosum and A. cepa, and four wild species, A. altaicum, A. galanthum, A. oschaninii, and A. vavilovii, in section Cepa were investigated based on RFLPs of mitochondrial DNA (mtDNA) and chloroplast DNA (cpDNA). Using the group average method, cluster analysis was performed in the six species and dendrograms of mt and cpDNAs were constructed. A. cepa formed a subcluster with A. vavilovii at small distance value in the dendrogram of mtDNA. Furthermore, these two species had no distance value each other in dendrograms of cpDNA. A. fistulosum formed subclusters with A. altaicum at small distance value in both dendrograms of mt and cpDNAs. These results represent the close relationships of A. cepa with A. vavilovii and of A. fistulosum with A. altaicum. Because of large distance value of A. oschaninii with other five species in dendrograms of mt and cpDNAs, A. oschaninii is considered to have a long genetic distance from others. This study demonstrates that phylogenetic relationships among the species on RFLPs of mtDNA closely correspond to those of cpDNA in section Cepa.
    Preview · Article · Jan 2001 · Engei Gakkai zasshi
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    Masayoshi Shigyo · Tomoya Miyazaki · Shiro Isshiki · Yosuke Tashiro
    [Show abstract] [Hide abstract] ABSTRACT: The chromosomal locations of randomly amplified polymorphic DNA (RAPD) markers were examined in shallot (Allium cepa L. Aggregatum group). A series of alien monosomic addition lines of Japanese bunching onion (A. fistulosum L.) with extra chromosomes (1A-8A) from A. cepa Aggregatum group was used as plant materials. Several decamer (OPERON KITs A, E, and G) and 12-mer (Wako DNA Oligomer sets A-1, C-4, F-4, and F-5) oligonucleotides were applied as random primers. The chromosomal locations of the 16 RAPD markers were determined; the RAPD marker OPE091600 was located on the chromosome 1A of A. cepa Aggregatum group, OPE03600 and OPE181400 on 2A, OPA12700 on 3A, OPA11950, OPE181500, OPG10800, WAA091800, WAF65900, and WAF83800 on 4A, OPE17500 and OPE182000 on 5A, OPG08500 on 6A, WAF812800 and WAF832200 on 7A, and WAC68500 on 8A. Consequently, the RAPD markers were assigned to all the chromosomes of A. cepa Aggregatum group.
    Full-text · Article · Aug 1997 · Genes & Genetic Systems