Mohammed Sayed

Assiut University, Lycopolis, Asyūţ, Egypt

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Publications (4)0 Total impact

  • Saber Kotb · Mohammed Sayed · Ahmed AbdelRady
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    ABSTRACT: The present study was concerned with 42 dwellings of lactating dromedary she-camels in owner's houses which distributed in 6 different villages around Assiut city, Egypt; to be representative to camel distribution in villages of Manfalout, El-kousia, El-Fateh, Abnob, Abo-Tig and Sedfa. This study was unlike most studies that deal with camels in the nomadic life while here all animals were traditionally managed and were housed in each dwelling. The environmental conditions around animals were estimated microbiologically to evaluate the hygiene of their habitat and to estimate milk quality and also to monitor subclinical mastitis. Therefore, different random samples of air, soil, water, milkers' hands, milk equipments, composite milk and bulk milk samples were collected. It was found wide varieties of a total 599 of pathogenic and potentially pathogenic isolates could be detected and identified in which soil samples had the highest number as 208, while composite milk samples had the lowest as 20. As the milk was subjected to the surrounding environment, the isolates were elevated to 113 as explained by the significance correlation of total bacterial count between bulk milk and soil. Although all animals were observed as clinically healthy with apparently normal udder, the CMT showed 9.52% was positive for subclinical mastitis.
    No preview · Article · Jun 2010
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    Article: Sanitary conditions of lactating dromedary she-camel environment with special reference to milk quality and subclinical mastitis monitoring ‫وﻣﺮاﻗﺒﺔ‬ ‫اﻟﻠﺒﻦ‬ ‫ﻟﺠﻮدة‬ ‫اﻟﺨﺎﺻﺔ‬ ‫اﻹﺷﺎرة‬ ‫ﻣﻊ‬ ‫اﻟﺤﻼﺑﺔ‬ ‫اﻟﺠﻤﻞ‬ ‫ﻧﺎﻗﺔ‬ ‫ﻟﺒﻴﺌﺔ‬ ‫اﻟﺼﺤﻴﺔ‬ ‫اﻟﻈﺮوف‬ ‫ﺗﺤﺖ‬ ‫اﻟﻀﺮع‬ ‫اﻟﺘﻬﺎب‬ ‫اﻹآﻠﻴﻨﻴﻜﻲ‬ ‫ﻗﻄﺐ‬ ‫ﺻﺎﺑﺮ‬ ‫ﺳﻴﺪ‬ ‫ﻣﺤﻤﺪ‬ ، 2 * ‫ﻣﺼﺮ‬ ،‫أﺳﻴﻮط‬ ‫ﺟﺎﻣﻌﺔ‬ ،‫اﻟﺒﻴﻄﺮي‬ ‫اﻟﻄﺐ‬ ‫آﻠﻴﺔ‬ ،‫اﻟﺤﻴﻮان‬ ‫ﺻﺤﺔ‬ ‫ﻗﺴﻢ‬ ‫ﺟﺎﻣﻌﺔ‬ ،‫اﻟﺒﻴﻄﺮي‬ ‫اﻟﻄﺐ‬ ‫آﻠﻴﺔ‬ ،‫اﻷﻏﺬﻳﺔ‬ ‫ﻋﻠﻰ‬ ‫اﻟﺼﺤﻴﺔ‬ ‫اﻟﺮﻗﺎﺑﺔ‬ ‫ﻗﺴﻢ‬ ‫ﻣﺼﺮ‬ ،‫أﺳﻴﻮط‬
    [Show abstract] [Hide abstract]
    ABSTRACT: The present study was concerned with 42 dwellings of lactating dromedary she-camels in owner's houses which distributed in 6 different villages around Assiut city, Egypt; to be representative to camel distribution in villages of Manfalout, El-kousia, El-Fateh, Abnob, Abo-Tig and Sedfa. This study was unlike most studies that deal with camels in the nomadic life while here all animals were traditionally managed and were housed in each dwelling. The environmental conditions around animals were estimated microbiologically to evaluate the hygiene of their habitat and to estimate milk quality and also to monitor subclinical mastitis. Therefore, different random samples of air, soil, water, milkers' hands, milk equipments, composite milk and bulk milk samples were collected. It was found wide varieties of a total 599 of pathogenic and potentially pathogenic isolates could be detected and identified in which soil samples had the highest number as 208, while composite milk samples had the lowest as 20. As the milk was subjected to the surrounding environment, the isolates were elevated to 113 as explained by the significance correlation of total bacterial count between bulk milk and soil. Although all animals were observed as clinically healthy with apparently normal udder, the CMT showed 9.52% was positive for subclinical mastitis.
    Full-text · Article · Jan 2010
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    Ahmed Abdel-Rady · Mohammed Sayed
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    ABSTRACT: In this investigation, some epidemiological studies were run on subclinical mastitis for totally 350 dairy cows of different breeds, ages and distributed in different villages in Assiut governorate, Assiut, Egypt, along a whole year (during the period from June 2006 till July 2007) through field screening surveys by using of the California mastitis test (CMT) for each quarter milk sample followed by bacteriological examination to identify the major causative agents of intramammary infection (IMI). The dairy cows were differed from the breed point of view as 230 Holstein Friesian breed and 120 native breed. Also, they were differed from the age point of view as a group of 95 cows aged from 2 to 4 years old and another group of 255 cow aged from 5 to 8 years old. All dairy cows were apparently healthy with clinically sound udder secreting apparently normal milk. All the cows lived nearly under the same conditions of breeding from the habitat, hygiene and feeding systems. The obtained results revealed that 67 cows (19.14%) had 80 infected quarters (5.71%). It was found that the most frequently major causative agents isolated were Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli from the positive CMT samples with prevalence 52.5, 31.25 and 16.25%, respectively. With studying the breed factor, it was found Friesian breed was sensitive towards infection (20.43% at the cow level and 6.09% at the quarter level) than of native breed (16.67% at the cow level and 5% at the quarter level). It was also noticed that the prevalence of subclinical mastitis in hot weather as during summer (9.14% at the cow level and 2.64% at the quarter level) and during spring (4.86% at the cow level and 1.36% at the quarter level) was higher than in cold weather as during winter (2% at the cow level and 0.64% at the quarter level) and during autumn (3.14% at the cow level and 1.07% at the quarter level). In relation to age susceptibility, 5-8 years old cows (15.43% at the cow level and 4.36% at the quarter level) were susceptible than those of 2-4 years (3.71% at the cow level and 1.36% at the quarter level). The degree of quarter attack according to positive CMT was varied from 35 quarters (2.50%) showed degree (+++), to 45 ones (3.22%) showed degree (++), to 120 ones (8.57%) showed degree (+) and the rest (85.71%) showed degree (-). The obtained results threw the light on the epidemiology of subclinical mastitis in Assiut villages and provided an importance of the CMT for diagnosis of subclinical mastitis due to it is a reliable, easy, rapid and cheap tool helping in diagnosis and controlling the disease because it directs attention to individual mammary quarter that is secreting milk of high somatic cell content (SCC). Programs for control of subclinical mastitis may be planned around the routine examination of all lactating cows, and consequently early treatment can be applied towards positive cases rapidly for preventing their conversion towards clinical form among dairy cows and for protecting the herd health, milk hygiene and consequently the consumer health.
    Full-text · Article · Jan 2009 · Veterinary World
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    Ahmed Abdel-Rady · Mohammed Sayed
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    ABSTRACT: In this investigation, hot & cold modified Ziehl-Neelsen (ZN) staining and direct smear (DS) methods were used for detection of Cryptosporidium oocysts in 55 fecal samples collected from 55 newly born calves, their ages varied from 1 week to 2 months old, suffered from severe diarrhea with signs of depression and some degree of dehydration without signs of hyperthermia. The calves of interest were admitted to the veterinary teaching hospital, Assiut University, for diagnosis and treatment. The fecal samples were examined using a light microscope by DS method, in which Cryptosporidium oocysts were detected in 2 samples with a percentage of 3.6%. The staining with cold modified ZN staining method detected the oocysts in 19 samples with a percentage of 34.5%. While, the hot modified ZN staining method detected the oocysts in 27 samples with a percentage of 49%. This investigation was run for evaluating of different methods for diagnosis of cryptosporidiosis and it was concluded that the staining methods were more accurate than DS method, in addition to the hot modified ZN staining was the most efficient method in detection of Cryptosporidium oocysts which diagnosed with a high percentage the degree of infestation, so it is recommended as efficient, rapid, easy and less costly method for diagnosis of cryptosporidiosis, and consequently the drug of choice for treatment and control of the disease and preventing drawbacks can be selected by applying the proper therapeutic plan and to prevent the economic losses in calves.
    Full-text · Conference Paper · Dec 2008