Laila Khaleda's scientific contributions
Publications (5)
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- Abstract: The rural and marginal people of Bangladesh are deprived of modern treatment facilities and hence greatly depend on medicinal plants. Besides, the higher cost and toxicity of synthetic drugs drives scientists towards search for natural source of medication for a number of diseases. Cost-effectiveness, easy availability and fewer side effects are making the herbal medicine more popular both among rural and city people. Plants with Cytotoxic and clot lysis potential are good candidate as... Show More
- Abstract: In recent years, bacterial infections are increasing due to emergence of antibiotic resistance. Oxidative stress and gall bladder stones also pose a great health threat to millions. This study attempted the assessment of antibacterial, anti-oxidant and anti-gall bladder stones activity of an important medicinal plant Achyranthes aspera methanolic leaf extract. Anti-bacterial activity was performed by disc diffusion method. Significant susceptibility was observed against gram positive... Show More
- Abstract: The current situation of medicinal plants and increasing demand of plant derived drugs suggest an immediate need to conserve our medicinal plant resources. The present study attempted to develop in vitro micropropagation protocol for the important medicinal plant Achyranthes bidentata Blume. Shoot tip and nodal segment of healthy plants from natural condition were used as explants for direct organogenesis. Varying concentrations of auxins and cytokinins both alone and in combination were... Show More
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Article: Protective Effect of Spirodela polyrhiza on Various Organs of Arsenicinduced Wistar Albino Rats
Abstract: Our study was undertaken to assess the protective effect of Spirodela polyrhiza against sodium arseniteinduced toxicity in experimental model. Animals were divided into six groups. The first group was used as control group while the other groups were treated with S. polyrhiza, sodium arsenite (10 ppm), 10 ppm As plus S. polyrhiza, sodium arsenite (30 ppm), and 30 ppm As plus S. polyrhiza respectively. Organs (kidney, spleen and heart) were collected at the end of the experiment for... Show More
Publications citing this author (4)
- The results of this study are based on some important parameters and factors like, responses of the culture, number and length of shoots and roots, intensity of callus, physical and chemical factors and microenvironment of the cultures. The results of the plant tissue culture experiments also depend on the plant species and their parts used as explants (Hossain et al., 2013). The nodal segments (explants) were collected from a 4 year old mature plant for this study.
[Show abstract] [Hide abstract] ABSTRACT: We report an improved in vitro propagation protocol for Morinda citrifolia L. in the present communication using nodal segments as explants from a 4 year old plant. Murashige and Skoog (MS) medium was used to inoculate the sterilized explants. Under laboratory conditions, 4.6 ± 0.48 shoots were regenerated from the nodal meristem of an explant on the combination of MS medium fortified with 4.0 mg/L 6-benzylaminopurine (BAP). Semi-solid (with agar) and liquid MS medium was used for the multiplication of shoots in vitro. Combined effect of BAP and kinetin in shoot multiplication was reported favorable in semi-solid (10.6 ± 0.17 shoots per vessel) and liquid MS medium (11.4 ± 0.47 shoots per vessel). The shoots were rooted in vitro on half-strength agar-gelled MS medium supplemented with 1.0 mg/L indole-3 butyric acid (IBA) and 44.30 roots per shoot were observed from the cut ends of the shoots. All the shoots were also rooted by ex vitro method and maximum 36.12 roots per shoot were induced by treating the shoots with 300 mg/L IBA for 5 min. The in vitro rooted and ex vitro treated plantlets were transferred to the soilrite containing paper cups for hardening in the greenhouse. The plantlets were shifted to nursery bags after 4 weeks. Finally the hardened plantlets were planted in the field with 100% rate of survival under natural conditions. The ex vitro rooting was reported more advantageous than in vitro rooting in terms of cost, time and percent survival of plantlets.- In the present study we have demonstrated the optimum culture conditions required for maximum secretion of antibacterial compounds by the endophytic fungal genus JAS-2, which was isolated from Achyranthus aspera, commonly known as latzeera, chichida, apamarga. Considering multiple medicinal properties of this plant such as cytotoxity [6] and wound healing [7], the plant was chosen for isolating biologically active endophytic fungi. Since crude metabolite consists only a meagre amount of bioactive product, it is extremely important to increase the amount of active compound.
[Show abstract] [Hide abstract] ABSTRACT: The present study explores the efficacy of an endophytic fungus from symbiotic plant Achyranthus aspera, an herb of medicinal importance. Considering limitation of secreted fungal metabolites in terms of biologically active molecules; an endeavour was made to increase the amount of secreted active product. Enhanced secretion of active compound was observed by optimizing different parameter of culture conditions. The fungal culture was isolated from stem of Achyranthus aspera and taxonomically identified as Aspergillus terreus. While analysing it's different in vitro potential, culture metabolites showed antibacterial, antifungal and anti-oxidant activity. In order to increase the yield of compound, culture was optimized for different parameters such as carbon and nitrogen sources and extracting solvent. All the optimization was performed based on % inhibition of bacterial growth when challenged with 10 μg/μl metabolite concentration. Among different media used, potato dextrose broth (PDB) and sabouraud's dextrose broth (SDB) proven to be better media for growth of fungus as well as metabolites production. 1% yeast extract and 4% dextrose resulted in higher cell inhibition. Ethyl acetate served as good extracting solvent.
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