[Show abstract][Hide abstract] ABSTRACT: A new genus of a deep-sea ascomycete with one new species, Alisea longicolla, is described based on analyses of 18S and 28S rDNA sequences and morphological characters. A. longicolla was found together with Oceanitis scuticella, on small twigs and sugar cane debris trawled from the bottom of the Pacific Ocean off Vanuatu Islands. Molecular and morphological characters indicate that both fungi are members of Halosphaeriaceae. Within this family, O. scuticella is phylogenetically related to Ascosalsum and shares similar ascospore morphology and appendage ontogeny. The genus Ascosalsum is considered congeneric with Oceanitis and Ascosalsum cincinnatulum, Ascosalsum unicaudatum and Ascosalsum viscidulum are transferred to Oceanitis, an earlier generic name.
Full-text · Article · Dec 2009 · Mycological Research
[Show abstract][Hide abstract] ABSTRACT: Restrictions patterns of the ITS rDNA and a partial fragment of the b-tubulin gene were shown to help identify Phaeoacremonium species associated with diseased grapevines. PCR-RFLP markers revealed a high frequency of P. parasiticum, together with P. aleophilum, in fungi isolated from stems and trunks of mature diseased vines showing ' hoja de malvon' disease, and from young declining plants in Argentina. This distribution is different from that in vineyards in other parts of the world, where P. aleophilum is dominant. Indeed, this is the first report associating P. parasiticum with diseased vines.
Full-text · Article · Oct 2002 · Mycological Research
[Show abstract][Hide abstract] ABSTRACT: A group of Phaeoacremonium isolates first distinguished by the red pigmentation of the reverse of their colonies, is described as a new species, P. viticola, on the basis of molecular data. An intensive sampling in French vineyards affected by the Esca disease has shown that this species is rare compared to P. aleophilum and P. chlamydosporum and is often associated with symptoms on the fruits.
[Show abstract][Hide abstract] ABSTRACT: A molecular approach combined with phenotypic and morphological observations was used to delimit Phaeoacremonium species involved in esca disease and grapevine declines. P. aleophilum and P. angustius were synonymised, a new species P. viticola was reported and preliminary results concerning the delimitation of P. inflatipes and P. parasiticum were presented. The incubation of isolates at 35°C appeared to be a discriminant character to identify these two species, P. inflatipes having very low growth in comparison with P. parasiticum. Specific restriction profiles were determined using the type strain of each species, and the method was applied to screen unidentified isolates.
[Show abstract][Hide abstract] ABSTRACT: The main goal of this work was to develop rapid and accurate molecular tools to discriminate species of white industrial Penicillia. We applied three different polymerase chain reaction (PCR) based techniques. Sequences of the ITS region of the rRNA gene unit and of the 5' end of the beta tubulin gene yielded 1.2% and 5.8% nucleotide variability respectively, between Penicillium camembertii and Penicillium nalgiovense. Polymorphic restriction sites were found in both sequences. These may be used in diagnostic PCR-RFLP analysis to rapidly distinguish between the two Penicillium species. Random amplified polymorphic DNA (RAPD) markers were also useful to differentiate these two species, but no polymorphism was found at the subspecific level, which evidenced a high level of homogeneity of the isolates studied. By means of these three techniques, the real identity of industrial strains of Penicillium chrysogenum and P. nalgiovense could be demonstrated. The comparison of these isolates with type strains of the two species suggested that the former corresponds to P. nalgiovense. The genetic relatedness between P. naglovense and Penicillium dipodomyis was also confirmed.
No preview · Article · Sep 1999 · International Journal of Food Microbiology