Tongxin Chen

Shanghai Jiao Tong University, Shanghai, Shanghai Shi, China

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Publications (8)53.09 Total impact

  • YingYing Jin · Wei Zhao · Tongxin Chen

    No preview · Article · Feb 2015 · Journal of Allergy and Clinical Immunology
  • Jing Wu · WeiFan Wang · Hui Zhang · Wei Zhao · Tongxin Chen

    No preview · Article · Feb 2015 · Journal of Allergy and Clinical Immunology
  • Tongxin Chen · Wei Zhao · ZhiQing Tian

    No preview · Article · Feb 2015 · Journal of Allergy and Clinical Immunology

  • No preview · Article · Feb 2015 · Journal of Allergy and Clinical Immunology
  • Youying Mao · Lei Yin · Hua Huang · Zhengyu Zhou · Tongxin Chen · Wei Zhou
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    ABSTRACT: Objectives: To analyse the clinical features of Henoch-Schönlein purpura (HSP) with or without nephritis in Chinese children and to determine the risk factors for renal involvement. Methods: Patient characteristics, clinical parameters and laboratory data were retrospectively analysed in patients with HSP with or without nephritis. Logistic regression analysis was used to identify the risk factors for renal involvement. Results: A total of 535 patients with HSP were included in the study. HSP nephritis occurred in 267 patients (49.9%), ranging from isolated haematuria in 5.2%, mild proteinuria in 77.5%, moderate proteinuria in 6.4% and severe proteinuria in 10.9% of cases. In 90% of the cases, nephritis developed within 1 week of HSP onset; 98.5% of the cases with nephritis developed the condition within 1 month. Risk factors for the development of nephritis were age ≥6 years, purpura on sites other than the lower limbs and the presence of occult blood in the stool. Conclusion: These results suggest that patients aged ≥6 years, or who have purpura on the upper limbs or face, or who have occult blood in the stool should be particularly monitored for signs of nephritis.
    No preview · Article · Jun 2014 · The Journal of international medical research
  • Jihong Qian · Li Wang · Xiangliang Yuan · Lei Wang · Tongxin Chen
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    ABSTRACT: Abstract Context: Intravenous immunoglobulin (IVIG) has been successfully applied in immune-related diseases of adults and neonates, such as human immunodeficiency virus (HIV) infection and systemic lupus erythematosus (SLE). Objective: This study aims to investigate the distinct impacts of IVIG on cultured dendritic cells (DCs) from newborn and healthy adult. Materials and methods: Blood samples were collected from eight full-term newborns and eight healthy adult volunteers. DCs from cord blood and peripheral blood were both cultured in the RPMI 1640 medium containing 10% fetal calf serum, 50 ng/ml granulocyte/macrophage colony-stimulating factor (GM-CSF) and 10 ng/ml recombinant human interleukin-4 (rhIL-4) for 5 d with therapeutic IVIG (20 mg/ml) or physiological IVIG (10 mg/ml). Lipopolysaccharides (LPSs, 1 μg/ml) were added on the fifth day to induce the maturation of immature DCs. The phagocytosis of monocytes, expression of MR (mannose receptor), CD14, CD1a, CD80, CD83, CD86 and MHC II were examined by flow cytometry. The expression of IL-4 mRNA was detected by RT-PCR, while IFN-γ, IL-12 and IL-10 were analyzed by enzyme-linked immunosorbent assay (ELISA) commercial kits. Results: IVIG of therapeutic dose inhibited the phagocytosis, differentiation and maturation of DCs, whereas physiological dose exhibited an accelerated role in vitro, especially on DCs from neonates, but aroused different effects on cytokine secretion. Discussion and conclusion: The different responses are generally due to immature immune system of neonate, which has a limit capacity to maintain immunity homeostasis. Modulation of DCs phagocytosis, differentiation, maturation and cytokine secretion by IVIG is of potential relevance to its dosage and immune status of patients.
    No preview · Article · Dec 2013 · Immunopharmacology and Immunotoxicology
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    ABSTRACT: To cite this article: Qian J, Zhu J, Wang M, Wu S, Chen T. Suppressive effects of intravenous immunoglobulin (IVIG) on human umbilical cord blood immune cells. Pediatr Allergy Immunol 2011; 22: 211–220. We investigated in vitro immunomodulatory effects of intravenous immunoglobulin (IVIG) on cord blood mononuclear cells (CBMNC), macrophages, dendritic cells and CD3+ T cells were isolated from umbilical venous blood. Cell proliferation used 3H-TdR incorporation, culture supernatants were assayed for cytokines using ELISA, and surface marker expressions were determined by flow cytometry. IVIG suppressed CBMNCs and CD3+T-cells proliferation, secretions of IL-10, INF-γ and TGF-β1, but not IL-4, and PHA-induced expressions of surface molecules (CD25, CD45RA and CD45RO), with more pronounced effects for CBMNCs. IVIG decreased cord blood (CB) macrophage phagocytosis and CD14, HLA-DR and CD86 expressions. IVIG increased CD14 expression and decreased MCH II expression for differentiation-stage CB dendritic cells (DCs) and increased CD14 expression and decreased CD80 and CD83 expressions of mature DCs, suggesting that IVIG intervention inhibited DC differentiation and maturation. In addition to T cells, IVIG immunomodulatory effects on CBMNCs involve a variety of cells and molecules. CB macrophages and CBMNC-DCs are targets of IVIG.
    No preview · Article · Sep 2010 · Pediatric Allergy and Immunology
  • Jihong Qian · Tongxin Chen · Weiming Lu · Shengmei Wu · Jianxing Zhu
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    ABSTRACT: The objective of this study was to measure and compare the macro- and micronutrient composition of breast milk in urban and suburban lactating mothers living in Shanghai, China. Primiparous women residing in three urban regions (n= 30/region) and a suburban area of Shanghai (n= 30) were recruited. Breast milk samples were collected 8-10 days postpartum and dietary intake was determined. Women from the suburban area were of lower socio-economic status as indicated by levels of education, occupation and income. With the exception of carbohydrates, mothers in all urban areas had significantly higher macronutrient intake levels than suburban area mothers. Sodium, potassium, phosphorus and calcium intake levels were significantly lower in suburban compared to all urban women. Concentrations of protein, lipids, copper, sodium, potassium, chlorine, zinc, manganese, phosphorus and iron were all significantly lower in milk obtained from suburban compared to urban women. Concentrations of carbohydrates in milk from suburban women were significantly higher than concentrations in than two of the three urban groups. Offspring from women in Chongming county gained significantly less weight between birth and 6 months than did offspring born to women from the three other counties. In summary, there were significant differences in macro- and micronutrient intake levels and breast milk concentrations between women from urban and suburban Shanghai. These differences may have important implications for the growth and development of breastfed infants.
    No preview · Article · Mar 2010 · Journal of Paediatrics and Child Health