Wei-Hong Jia

Academy of Military Medical Sciences, T’ien-ching-shih, Tianjin Shi, China

Are you Wei-Hong Jia?

Claim your profile

Publications (2)1.38 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Six internal protein gene segments of attenuated, cold-adapted(ca), temperature -sensitive (ts) influenza A/Ann Arbor/6/60 ca (H2N2) and HA, NA gene segments of A/California/07/2009ca were introduced to plasmid pAD3000 carrying pol I and pol II promoters, and rescued the reassortant virus from Vero cell using reverse genetics technology The reassortant has the attenuate characters, ca and ts, the TCID5(0) is 7 5, HA titer maintain at 1 256 and EID50 is 8 which was detected using SPF eggs The stable of reassortant is determined by RT-PCR gene segments from virus which were propagated in eggs The morphology of reassortant conform the wild type virus In order to test the immunogenicity, the reassortant viruses were purified Mice were intranasally immunized and intramuscular injected with inactive whole virus as control The high HI titer can be detected in both groups, seems hinger in i m (intramuscular) immunized groups (P=0 044), but higher IgA titer can only detected in in (intranasal) immunized group Compared with i m immunized group, higer pro-inflammation cytokines IL-1 beta, TNF alpha, IFN-alpha were tested in in groups, means faster and stronger mucosal immune response was induced Virus load were detected in lung, brain, spleen 4 days after immunization to determine the safety of reassortant as vaccine candidate, no detectable virus were found All results show that the vaccine candidate can be used for vaccine production
    No preview · Article · Dec 2011 · Progress in Biochemistry and Biophysics
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: H3N2 subtype influenza A viruses have been identified in humans worldwide, raising concerns about their pandemic potential and prompting the development of candidate vaccines to protect humans against this subtype of influenza A virus. The aim of this study was to establish a system for rescuing of a cold-adapted high-yielding H3N2 subtype human influenza virus by reverse genetics. In order to generate better and safer vaccine candidate viruses, a cold-adapted high yielding reassortant H3N2 influenza A virus was genetically constructed by reverse genetics and was designated as rgAA-H3N2. The rgAA-H3N2 virus contained HA and NA genes from an epidemic strain A/Wisconsin/67/2005 (H3N2) in a background of internal genes derived from the master donor viruses (MDV), cold-adapted (ca), temperature sensitive (ts), live attenuated influenza virus strain A/Ann Arbor/6/60 (MDV-A). In this presentation, the virus HA titer of rgAA-H3N2 in the allantoic fluid from infected embryonated eggs was as high as 1:1024. A fluorescent focus assay (FFU) was performed 24-36 hours post-infection using a specific antibody and bright staining was used for determining the virus titer. The allantoic fluid containing the recovered influenza virus was analyzed in a hemagglutination inhibition (HI) test and the specific inhibition was found. The results mentioned above demonstrated that cold-adapted, attenuated reassortant H3N2 subtype influenza A virus was successfully generated, which laid a good foundation for the further related research.
    Full-text · Article · Dec 2009 · Chinese medical journal