[Show abstract][Hide abstract] ABSTRACT: The combination of levofloxacin and α(1) adrenergic antagonist treatment is the current preferred choice for both bacterial and non-bacterial prostatitis. The aim of this study is to explore the influence of α(1) adrenergic antagonists on the pharmacokinetics of levofloxacin using rat models with acute bacterial prostatitis (ABP) induced by direct injection with Escherichia coli (ATCC25922). A total of 96 model rats were randomly assigned into two groups: the experimental group (treated with both tamsulosin and levofloxacin, n=48) and the control group (treated with levofloxacin and solvents, n=48). Six rats from each group were euthanized to collect blood, liver, kidney and prostate samples at the time points of 0.125, 0.25, 0.5, 1, 2, 4, 8 and 12 h after drug administration. The levofloxacin concentrations were detected by high performance liquid chromatography (HPLC), and the pharmacokinetic parameters were calculated using the 3p97 software program. There were no obvious differences (P>0.05) between the experimental and control groups in the major pharmacokinetic parameters of levofloxacin, including the halftime (t(1/2)), time to peak (t(peak)), clearance rate (CL), maximum concentration (C(max)) and area under the curve (AUC(0∼12)), in the plasma or in the hepatic and kidney tissues of the model rats. However, in the prostatic tissues, tamsulosin increased the C(max), prolonged the t(1/2) and decreased the CL of levofloxacin (P<0.05). These results indicate that tamsulosin may enhance the effect of levofloxacin in the treatment of bacterial prostatitis without changing the drug concentration in the liver and kidney.Asian Journal of Andrology advance online publication, 28 January 2013; doi:10.1038/aja.2012.134.
Preview · Article · Jan 2013 · Asian Journal of Andrology
[Show abstract][Hide abstract] ABSTRACT: The accumulation of foam cells in atherosclerotic lesions is a hallmark of early-stage atherosclerosis. Kaempferol has been shown to inhibit oxidized low-density lipoprotein (oxLDL) uptake by macrophages; however, the underlying molecular mechanisms are not yet fully investigated. In this study, we shown that treatment with kaempferol markedly suppresses oxLDL-induced macrophage foam cell formation, which occurs due to a decrease in lipid accumulation and an increase in cholesterol efflux from THP-1-derived macrophages. Additionally, the kaempferol treatment of macrophages led to the downregulation of cluster of differentiation 36 (CD36) protein levels, the upregulation of ATP-binding cassette (ABC) transporter A1 (ABCA1), scavenger receptor class B type I (SR-BI) and ABCG1 protein levels, while no effects on scavenger receptor A (SR-A) expression were observed. Kaempferol had similar effects on the mRNA and protein expression of ABCA1, SR-BI, SR-A, CD36 and ABCG1. The reduced CD36 expression following kaempferol treatment involved the inhibition of c-Jun-activator protein-1 (AP-1) nuclear translocation. The inhibition of AP-1 using the inhibitor, SP600125, confirmed this involvement, as the AP-1 inhibition significantly augmented the kaempferol-induced reduction in CD36 expression. Accordingly, the kaempferol-mediated suppression of lipid accumulation in macrophages was also augmented by SP600125. The increased expression of ABCA1, SR-BI and ABCG1 following kaempferol treatment was accompanied by the enhanced protein expression of heme oxygenase-1 (HO-1). This increase was reversed following the knockdown of the HO-1 gene using small hairpin RNA (shRNA). Moreover, the kaempferol-mediated attenuation of lipid accumulation and the promotion of cholesterol efflux was also inhibited by HO-1 shRNA. In conclusion, the c-Jun-AP‑1-dependent downregulation of CD36 and the HO-1-dependent upregulation of ABCG1, SR-BI and ABCA1 may mediate the beneficial effects of kaempferol on foam cell formation.
Preview · Article · Dec 2012 · International Journal of Molecular Medicine
[Show abstract][Hide abstract] ABSTRACT: To study the dynamic changes of levels of serum interleukin-18 (IL-18) and tumor necrosis factor-alpha (TNF-alpha) in children with infectious mononucleosis (IM).
72 patients with IM were analyzed for the expression of TNF-alpha and IL-18 by RIA and ELISA, respectively in acute phase, convalescent phase, (the first month, the third month, the sixth month), and the control group.
The levels of serum TNF-alpha and IL-18 in patients with IM were significantly higher than that in control (P < 0.05). The serum levels of TNF-alpha and IL-18 decreased with time and in patients at stage were significantly higher than that in control (P < 0.05). The levels of serum cytokine were correlated with the state of illness.
The serum cytokine TNF-alpha and IL-18 may be regarded as index to reflect the state of immunity and prognosis of patients with IM.
No preview · Article · May 2010 · Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
[Show abstract][Hide abstract] ABSTRACT: To investigate correlation between the expression of survivin and caspase-3 proteins in juvenile laryngeal papilloma.
The expression of survivin and caspase-3 proteins were detected with immunohistochemical method in 43 cases of juvenile laryngeal papilloma, 25 vocal nodules and 25 normal laryngeal mucosa.
The positive rates of survivin protein in juvenile laryngeal papilloma were 57.14% and higher than that in vocal nodules (P<0.01) and the normal laryngeal mucosa (P<0.01). And the Caspase-3 protein positive rate was 26.19% in juvenile laryngeal papilloma and lower than that in vocal nodules and the normal laryngeal mucosa (P<0.01). There was a significant negative correlation between the expression of survivin and caspase-3 in juvenile laryngeal papilloma.
The abnormal expression of survivin and caspase-3 may play important role in the pathogenesis of juvenile laryngeal papilloma.
No preview · Article · Nov 2009 · Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
[Show abstract][Hide abstract] ABSTRACT: To explore the pharmacokinetics and bioequivalence of two kinds of enteric coated tablet of Zhengqing Fengtongning.
A single dose of 45 mg kg(-1) test or reference preparation was administrated by randomized crossover way in 12 rabbits. The plasma concentrations of drug were determined by HPLC. The pharmacokinetics parameters and relative bioequivalence were calculated with 3p97 program.
The concentration curves based on drug-time of both test and control preparations were presented by one-compartment model, tmax were (0.81 +/- 0.34), (0.60 +/- 0.30) h respectively, Cmax were (11.16 +/- 0.58), (11.90 +/- 1.44) microg mL(1) respectively, AUC(0-->t) were (61.58 +/- 6.70), (60.56 +/- 6.67) microg h mL(-1) respectively, relative bioavailability was (102.77% +/- 15.63)%. Suggesting no significant diffirence between the main pharmacokinetic parameters of two prepations.
The two preparations are bioequivalent.
No preview · Article · Mar 2008 · Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica