[Show abstract][Hide abstract] ABSTRACT: The three-dimensional (3D) biochips prepared in this study are composed of a glass microscopy slide arrayed with amino aerogel dots. The amino aerogel was produced using the sol-gel process, with an ionic liquid as the template followed by a solvent extraction to remove the template and build a three-dimensional mesoporous structure. The FTIR spectrum verified that the major template was removed and the (29)Si solid-state NMR spectra recognized the cross-linkages in the SiO(2) network structure. SEM images measured the particles at around 100 nm. After grinding, the BET analysis confirmed that the nano-size amino aerogel powders had exhibited specific surface area of 188 m(2)/g, pore volume of 0.83 cm(3)/g, and average pore size of 16.2 nm. The as-prepared amino aerogel surface contained amino functional groups capable of performing a sandwich immunoassay. The primary antibody was immobilized on the internal surface of the arrayed amino aerogel to capture its affinity antigen. On the top of the captured antigen, the report antibody was read its labeling fluorescent dye. In comparison to the corresponding two-dimensional (2D) biochip, the 3D amino aerogel biochips were observed to amplify signal intensities more effectively due to their remarkable capturing capability.
[Show abstract][Hide abstract] ABSTRACT: A nano-porous aerogel was produced in regular atmospheric conditions using the sol-gel polymerization of tetraethyl orthosilicate
(TEOS) to build a three-dimensional (3D) structure for recognizing nucleotide acids. The Fourier transformation infrared spectroscopy
and Brunauer-Emmett- Teller instrument had been used to characterize this 3D aerogel and concluded that it had a high porosity
and large internal networking surface area to capture nucleotide acids. The functionality of molecular recognition on nucleotide
acids was demonstrated on human gene ATP5O.
KeywordsAerogel–molecular recognition–DNA detection–3D biochips
[Show abstract][Hide abstract] ABSTRACT: Mesoporous aerogel was produced under regular atmospheric conditions using the sol-gel polymerization of tetraethyl orthosilicate with an ionic liquid as both solvent and active agent. This was then used to build a three-dimensional structure to recognize nucleotide acids. Fourier transformation infrared spectroscopy, scanning electron microscopy, (29)Si solid-state nuclear magnetic resonance, and Brunauer-Emmett-Teller instruments were used to characterize this 3D aerogel, demonstrating that it had high porosity and large internal networking surface area that could capture nucleotide acids. The functionality of molecular recognition on nucleotide acids was demonstrated by immobilizing an oligonucleotide to probe its DNA target and confirming the tagged fluorescent signals by confocal laser scanning microscopy. The results indicated that the as-prepared 3D bioaerogel was capable of providing a very large surface area to capture and recognize human gene ATP5O.
Full-text · Article · Oct 2009 · Acta biomaterialia