Zongsuo Liang

Zhejiang Sci-Tech University, Hang-hsien, Zhejiang Sheng, China

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Publications (62)119.09 Total impact

  • Xiaoru Zhang · Juane Dong · Hailong Liu · Jiao Wang · Yuexin Qi · Zongsuo Liang
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    ABSTRACT: Salvia miltiorrhiza is a traditional Chinese herbal medicine, whose quality and yield are often affected by diseases and environmental stresses during its growing season. Salicylic acid (SA) plays a significant role in plants responding to biotic and abiotic stresses, but the involved regulatory factors and their signaling mechanisms are largely unknown. In order to identify the genes involved in SA signaling, the RNA sequencing (RNA-seq) strategy was employed to evaluate the transcriptional profiles in S. miltiorrhiza cell cultures. A total of 50,778 unigenes were assembled, in which 5,316 unigenes were differentially expressed among 0-, 2-, and 8-h SA induction. The up-regulated genes were mainly involved in stimulus response and multi-organism process. A core set of candidate novel genes coding SA signaling component proteins was identified. Many transcription factors (e.g., WRKY, bHLH and GRAS) and genes involved in hormone signal transduction were differentially expressed in response to SA induction. Detailed analysis revealed that genes associated with defense signaling, such as antioxidant system genes, cytochrome P450s and ATP-binding cassette transporters, were significantly overexpressed, which can be used as genetic tools to investigate disease resistance. Our transcriptome analysis will help understand SA signaling and its mechanism of defense systems in S. miltiorrhiza.
    No preview · Article · Jan 2016 · PLoS ONE
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    Wanli Guo · Hussain Nazim · Zongsuo Liang · Dongfeng Yang
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    ABSTRACT: Although magnesium (Mg) is one of the most important nutrients, involving mainly in many enzyme activities and the structure stabilization of tissues, it was recognized as a forgotten macronutrient ion by botanists and agriculturists in the last decades, because that they did not regard Mg deficiency (MGD) in plants as a severe problem to our health. However, recent studies surprisingly showed that Mg contents in historical cereal seeds significantly declined with time, and 2/3 people surveyed in developed countries could not uptake enough Mg daily. Therefore, the response mechanisms to the MGD and how to increase Mg contents in plants may be two urgent realistic problems. In this review, we discussed several aspects of the MGD in plants, including the phenotypic and physiological changes, the Mg2+ homeostasis in cells controlled by Mg2+ transporters, the signaling of the MGD, the interactions between Mg2+ and other ions, and Mg’s roles in plant second metabolisms. We hope that these will improve us to understand the effluence of the MGD on plant growth and development, and will help crop breeding for Mg enrichment.
    Full-text · Article · Dec 2015
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    ABSTRACT: Four Panax genus species (Panax notoginseng (PN), Panax stipuleanatus (PS), Panax vietnamensis (PV) and Panax japonicus (PJ)) are all distributed in Southeast Asia. Besides PN, the other three species attracted little attention because of their limited distribution area, less wide application or relatively low quantity. PN had been cultivated as an industrial crop for hundreds years in China while the other three species as substitutes for PN in minority ethnic groups had no large-scale cultivation until now. The saponins of the four medical plants had been investigated in previous study while there has been virtually no reports describing their other compounds. In this study, the gas chromatography-mass spectrometry (GC–MS) was used to compare the volatile oils compounds of the four species. A total of 146 compounds were identified among all the species and every species contained compounds with similar quantity (all about 70). The falcarinol (28.86%) and andrographolide (38.35%) were the main compositions for PN. Furthermore, falcarinol was the dominant composition for PV (70.65%) and PS (64.61%), while pentadecanoic acid and ledene oxide-(II) were the high content compounds for PJ, accounting 9.31% and 8.39%, respectively. In addition, hierarchical clustering analysis was conducted to evaluate the relationship between the four Panax species based on contents (%) of chemical constituents in volatile oils. The results indicated that the PV and PS exhibited high similarity of volatile oils compounds. The PN, PV and PS could be used as the raw materials of falcarinol presenting well biological activities.
    No preview · Article · Nov 2015 · Industrial Crops and Products
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    ABSTRACT: Salvia miltiorrhiza, a popular traditional Chinese medicine, is widely used for treatments in cardiotonic disease. Tanshinones are a group of bioactive ingredients in S. miltiorrhiza. In this study, Ce3+ was used as an elicitor to enhance tanshinones production in S. miltiorrhiza hairy roots. The results showed that contents of dihydrotanshinone I (DTI) and cryptotanshinone (CT) were significantly enhanced by 50 μmol/L Ce3+, and reached to 0.875 mg/g and 0.271 mg/g, respectively. However, tanshinone II A (TIIA) and tanshinone I (TI) contents were significantly decreased to 59% and 62% of the control. Simultaneously, expressions of genes (HMGR, DXR, DXS1, DXS2 and GGPPS) involved in tanshinone biosynthesis were upregulated by Ce3+. Responses of DXS1, DXS2 and GGPPS to Ce3+ treatments were later than HMGR and DXR. We speculated that branch pathways of DTI and CT biosynthesis were probably different from TIIA and TI. This work will help us understand biosynthetic mechanism of tanshinones in plants.
    No preview · Article · Nov 2015 · Journal of Rare Earths
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    Yancui Wang · Feng Liu · Zongsuo Liang
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    ABSTRACT: Ophiopogon japonicus tubers have been widely used as food and traditional Chinese medicine in China. However, their nutritional composition has not been fully reported yet. This study aimed to analyze the nutritional composition of O. japonicus tubers. The α -glucosidase inhibitory and antioxidant activities of the extracts obtained from O. japonicus tubers were also evaluated by in vitro assays. The results indicated that O. japonicus tubers are rich in carbohydrates, proteins, minerals, and amino acids. Among four extracts, the n-butanol fraction (nBF) and chloroform/methanol extract (CME) of O. japonicus tubers had high amounts of total phenolic and flavonoid contents and exhibited good α -glucosidase inhibitory and antioxidant activities. The α -glucosidase inhibition of nBF was higher than acarbose. Overall, O. japonicus tubers are full of nutritional compounds and have good α -glucosidase inhibitory and antioxidant activities.
    Preview · Article · Oct 2015
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    ABSTRACT: Salvia miltiorrhiza is an important traditional Chinese herb in China and some other Asian countries. Phenolic acids are bioactive compounds in S. miltiorrhiza, the biosynthesis of which shares the general phenylpropanoid pathway with flavonoids. Chalcone synthase (CHS) is the entry point enzyme of the flavonoid pathway and plays a crucial role in flavonoid biosynthesis. In this study, the transcript of CHS was suppressed through RNAi-mediated silencing, and salicylic acid (SA) was added to the CHS silencing lines and wild-type lines as elicitor to investigate effects of the genetic modification and/or elicitor treatment on the production of phenolic acids in S. miltiorrhiza hairy roots. The results showed that the silencing of CHS enhanced contents of phenolic acids and decreased the accumulation of total flavonoids. The contents of phenolic acids in the SA treated CHS silencing lines were much higher than that in the CHS silencing lines and the SA treated wild-type lines. Our results imply that the substrate flow is directed to the phenolic acids pathway when the flavonoid pathway is interrupted, and combining the genetic modification and elicitor treatment is a new way to improve the production of phenolic acids in S. miltiorrhiza hairy root cultures.
    No preview · Article · Aug 2015 · Biochemical Engineering Journal
  • Bo Li · Chenlu Zhang · Liang Peng · Zongsuo Liang · Xijun Yan · Yonghong Zhu · Yan Liu
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    ABSTRACT: Salvia is an important genus containing nearly 1000 species of the Labiatae family; most species of Salvia are traditional herbal medicines and industrial materials with various active components. Salvia is also evidently rich in essential oils and phenolic acids. This study aimed to investigate the chemical composition of the essential oils and the content of phenolic acids in four Salvia species cultivated in Yangling, PR China. The essential oils from the fresh leaves and flowers of Salvia miltiorrhiza, Salvia przewalskii, Salvia officinalis, and Salvia deserta were obtained by simultaneous distillation extraction and evaluated by GC–MS; the phenolic acids were analyzed by HPLC. Essential oil derived from S. deserta flowers contained at most 72 compounds, while 11 compounds were common to all Salvia oils. Aromadendrene oxide-(1) (8.3%) and β-caryophyllene (11.05%) were the major constituents in essential oils from S. miltiorrhiza leaves and flowers, respectively. The limonene content in leaf oil markedly differed from that of flower oil from S. przewalskii; thujone was the predominant component in both the leaf and flower oils of S. officinalis. Moreover, β-phellandrene (29.74%) was the most abundant compound in the essential oil of S. deserta flowers. Composition of essential oils from these Salvia species showed strong tissue and organ specificity. Furthermore, the contents of ferulic acid, caffeic acid and rosmarinic acid in the aerial parts of these species were also investigated. The leaves of S. przewalskii and flowers of S. officinalis were rich in rosmarinic acid, with contents of 64.1 ± 3.9 and 53.4 ± 2.9 mg/g dry weight, respectively. Thus, S. przewalskii leaves could be considered a good source of rosmarinic acid.
    No preview · Article · Jul 2015 · Industrial Crops and Products
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    ABSTRACT: Nitric oxide (NO), a well-known signaling molecule plays an important role in abiotic and biotic stress-induced production of plant secondary metabolites. In this study, roles of NO in water stress-induced tanshinone production in Salvia miltiorrhiza hairy roots were investigated. The results showed that accumulations of four tanshinone compounds in S. miltiorrhiza hairy roots were significantly stimulated by sodium nitroprusside (SNP, a NO donor) at 100 μM. Effects of SNP were just partially arrested by the mevalonate (MVA) pathway inhibitor (mevinolin), but were completely inhibited by the 2-C-methyl-d-erythritol-4-phosphate pathway (MEP) inhibitor (fosmidomycin). The increase of tanshinone accumulation and the up-regulation of HMGR and DXR expression by PEG and ABA treatments were partially inhibited by an inhibitor of NO biosynthesis (Nω-nitro-L-arginine methyl ester (L-NAME)) and a NO scavenger (2-(4-Carboxyphenyl)- 4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO)). Simultaneously, NO generation in the hairy roots was triggered by PEG and ABA, and the effects were also arrested by c-PTIO and L-NAME. These results indicated that NO signaling probably plays a central role in water stress-induced tanshinone production in S. miltiorrhiza hairy roots. SNP mainly stimulated the MEP pathway to increase tanshinone accumulation.
    No preview · Article · May 2015 · Molecules
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    ABSTRACT: Background: Blind and excessive application of fertilizers was found during the cultivation of Panax notoginseng in fields, as well as increase in root rot disease incidence. Methods: Both "3414" application and orthogonal test designs were performed at Shilin county, Yunnan province, China, for NPK (nitrogen, phosphorus, and potassium) and mineral fertilizers, respectively. The data were used to construct the one-, two-, and three-factor quadratic regression models. The effect of fertilizer deficiency on root yield loss was also analyzed to confirm the result predicted by these models. A pot culture experiment was performed to observe the incidence rate of root rot disease and to obtain the best range in which the highest yield of root and saponins could be realized. Results: The best application strategy for NPK fertilizer was 0 kg/667 m(2), 17.01 kg/667 m(2), and 56.87 kg/667 m(2), respectively, which can produce the highest root yield of 1,861.90 g (dried root of 100 plants). For mineral fertilizers, calcium and magnesium fertilizers had a significant and positive effect on root yield and the content of four active saponins, respectively. The severity of root rot disease increased with the increase in soil moisture. The best range of soil moisture varied from 0.56 FC (field capacity of water) to 0.59 FC, when the highest yield of root and saponins could be realized as well as the lower incidence rate of root disease. Conclusion: These results indicate that the amount of nitrogen fertilizer used in these fields is excessive and that of potassium fertilizer is deficient. Higher soil moisture is an important factor that increases the severity of the root rot disease.
    Preview · Article · Apr 2015 · Journal of ginseng research
  • Yonghong Liu · Wenting Liu · Zongsuo Liang
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    ABSTRACT: Pinellia ternata (Thunb.) Berit., a perennial herb belonging to Araceae, is one of the few medicinal plants to produce purine alkaloids. It is speculated that endophytic bacteria from P. ternata may produce guanosine or inosine. However, there is no report about endophytic bacteria in P. ternata. In this study, endophytic bacteria were isolated from P. ternata and examined for the first time. This study finds a novel way to increase the yield of P. ternata herb, and to provide some new alkaloid producers. Plant material includes leaves, tubers, and roots of cultivated and wild P. ternata. The dilutions were smeared onto beef extract-peptone medium and cultured at 28 °C in darkness for 48-72 h. Co-culture treatments were prepared by inoculating 100 mL liquid 1/2 MS medium with bacterial culture broth at concentrations of 0 (control), 0.5%, and 1.5% (v/v). Of the 34 endophytic bacterial colonies isolated from P. ternata leaves, roots, and tubers, five strains were able to produce purine alkaloids. Results from 16s rDNA sequence analysis indicated that the bacteria belonged to Bacillus cereus, Aranicola proteolyticus, Serratia liquefaciens, Bacillus thuringiensis, and Bacillus licheniformis. Co-culture with living Serratia liquefaciens cells increased PLB growth by 58-71%. Co-culture with living Bacillus licheniformis cells increased PLB growth by 4-11%. This study provides a novel way for improving the yield of P. ternata herb, and for the production of purine alkaloids by the fermentation industry.
    No preview · Article · Apr 2015 · Pharmaceutical Biology
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    ABSTRACT: Magnesium (Mg) is one of the most important nutrients, involves mainly in plant growth and development. However, the signaling pathways response to magnesium stresses (MgSs) is known little, but several studies lately may improve the research development. Several phytohormones such as abscisic acid (ABA), ethylene, auxins, and their factors were found responding to MgSs, and there may be some signal pathways linking these hormones and downstream reactions together, e.g., carbon fixation and transfer, starch and sugar metabolism, ion uptaking and reactive oxygen species (ROS) increasing. Consequently, Arabidopsis morphogenesis is remodeled. In this review, we mainly discussed recent literatures involving in plant response to MgSs (Mg deficiency (MgD) and Mg toxicity (MgT)), including plant morphogenesis remodeling, magnesium transporters and signaling transductions. Moreover, the future study challenges related to the responding signalings to MgSs in plants are also presented. Regardless, the iceberg of signal transduction of MgSs in plants is appeared.
    No preview · Article · Mar 2015 · Plant signaling & behavior
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    ABSTRACT: Salvia castanea Diels f. tomentosa Stib. is an endemic medicinal plant distributed in China, and the notably high content of tanshinone IIA in the root is proven effective for the therapy of heart diseases. Hairy root induction of this Salvia species was inoculated with Agrobacterium rhizogenes strain ATCC 15834. Transformed hairy root was cultured in 6,7-V liquid medium for growth kinetics assessment and elicitation. An S curve was present in the hairy root cultures based on the fresh and dry weights with an interval of 3 days. An optimum concentration of the applied elicitors (15 μM Ag(+), 200 μM methyl jasmonate, and 200 mg l(-1) yeast extract elicitor) benefitted both the growth status and tanshinone accumulation in the hairy root cultures. Tanshinone IIA contents were mostly stimulated 1.8-fold and 1.99-fold compared with the control by Ag(+) and methyl jasmonate elicitation, respectively. Yeast extract dramatically enhanced dry mass accumulation, while it promoted cryptotanshinone content of 2.84 ± 0.33 mg g(-1) dry weight at most in the hairy root cultures. Selected elicitors diversely influenced tanshinone accumulation in the time courses of hairy root cultures within 7 days. Furthermore, transcripts of selected genes in the tanshinone biosynthetic pathway were remarkably upregulated with elicitation. Yeast extract elicitor heightened 13.9-fold of isopentenyl diphosphate isomerase expression level at 12 h, while it increased 16.7-fold of geranylgeranyl diphosphate synthase transcript at 24 h compared with that of the control, which was more effective than Ag(+) and methyl jasmonate. This study provided a convenient hairy root culture system of S. castanea Diels f. tomentosa Stib. for tanshinone production for the first time.
    No preview · Article · Mar 2015 · Protoplasma
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    Xuhong Du · Yulong Zhao · Dongfeng Yang · Yan Liu · Kai Fan · Zongsuo Liang · Ruilian Han
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    ABSTRACT: Panax notoginseng (Burk.) F.H. Chen (commonly known as Tianqi or Sanqi) is a famous traditional Chinese medicine and has been widely used for treatment in cardio- and cerebro-vascular diseases. However, the quality assessment of Sanqi is quite difficult because of ingredients complexity. In this work, the chemical fingerprints of 15 batches of P. notoginseng from different sources were investigated by ultra-performance liquid chromatography (UPLC). Fourteen common peaks in these samples were detected including 20(S)-protopanaxatriol saponins (PTS) and 20(S)-protopanaxadiol saponins (PDS). These samples were divided into three clusters by hierarchical clustering analysis (HCA). Cluster I and III possessed stronger anti-coagulation effects than cluster II. Multiple linear regression analysis (MLRA) showed that notoginsenoside R1, ginsenoside Rb1, ginsenoside Rd and an unknown compound might be the major effective compoounds for the quality control of Sanqi. PDS (Rb1, Rd, and the unknown compound) possesses a stronger anticoagulant activity than PTS (R1 only). This conjecture was confirmed by the dose–effect relationship evaluation of PDS and PTS. This study provides a general correlation model of fingerprint and efficacy relationship for the quality control of Sanqi and other TCMs.
    Preview · Article · Feb 2015 · Analytical methods
  • Pengda Ma · Jingying Liu · Anne Osbourn · Juane Dong · Zongsuo Liang
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    ABSTRACT: Salvia miltiorrhiza (Chinese name: dānshēn) is used in traditional Chinese medicine for the treatment of cardiovascular and cerebrovascular diseases. The tanshinones represent the most important biological active class of compounds present in dānshēn extracts. They are synthesized via either the cytoplasmic mevalonate or the plastidial 2-C-methyl-D-erythritol-4-phosphate pathway. Here, we summarize recent discoveries regarding the mechanisms underlying tanshinone biosynthesis and how the process is regulated. Tanshinone accumulation in planta is affected by a range of elicitors and by the composition of the culture medium. Its production in hairy root cultures can be enhanced by the over-expression of genes encoding 1-deoxy-D-xylulose 5-phosphate synthase, 3-hydroxy-3-methylglutaryl-CoA reductase, geranylgeranyl diphosphate synthase and allene oxide cyclase. The pathway leading to the biosynthesis of the tanshinone precursors miltiradiene and ferruginol, has been engineered in yeast.
    No preview · Article · Feb 2015 · RSC Advances
  • Zongsuo Liang · Wenting Liu · Xuefeng Feng · Guanghong Cui
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    ABSTRACT: Danshen leaves include cotyledon, true leaves , and compound leaves . The cotyledons are leaves that first come out of the ground during the germination of Danshen seeds .
    No preview · Article · Jan 2015
  • ShaoXuan HE · RuiLian HAN · ZongSuo LIANG

    No preview · Article · Jan 2015 · Chinese Journal
  • Zongsuo Liang · Wenting Liu
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    ABSTRACT: Danshen’s seeds are very small. The thousand seed’ weight is 1.64 g and the germination rate is about 70%. The germination rate decreases as the storage time prolongs. There are two ways of propagation by seeds: directly sowing and seedling transplantation. © Springer Science+Business Media Dordrecht and People’s Medical Publishing House 2015.
    No preview · Article · Jan 2015
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    ABSTRACT: Phenolic acids and tanshinones are two groups of bioactive ingredients in Salvia miltiorrhiza Bunge. As a heavy metal elicitor, it has been reported that Ag+ can induce accumulations of both phenolic acids and tanshinones in S. miltiorrhiza hairy roots. In this study, the effects of Ag+ treatment on accumulations of six phenolic acids and four tanshinones in S. miltiorrhiza hairy roots were investigated. To further elucidate the molecular mechanism, expressions of key genes involved in the biosynthesis of these ingredients were also detected. The results showed that although the total phenolic acids content was almost not affected by Ag+, accumulations of rosmarinic acid (RA), caffeic acid and ferulic acid were significantly increased, while accumulations of salvianolic acid B (LAB), danshensu (DSU) and cinnamic acid were decreased. We speculate that LAB probably derived from the branch pathway of DSU biosynthesis. Contents of four tanshinones were enhanced by Ag+ and their accumulations were more sensitive to Ag+ than phenolic acids. Genes in the upstream biosynthetic pathways of these ingredients responded to Ag+ earlier than those in the downstream biosynthetic pathways. Ag+ probably induced the whole pathways, upregulated gene expressions from the upstream pathways to the downstream pathways, and finally resulted in the enhancement of ingredient production. Compared with phenolic acids, tanshinone production was more sensitive to Ag+ treatments. This study will help us understand how secondary metabolism in S. miltiorrhiza responds to elicitors and provide a reference for the improvement of the production of targeted compounds in the near future.
    Preview · Article · Dec 2014 · Molecules
  • Dongfeng Yang · Xuhong Du · Zongqi Yang · Zongsuo Liang · Zhixin Guo · Yan Liu
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    ABSTRACT: Plant secondary metabolites play an important role in the fields of food, medicine, agriculture and biofuels. Secondary metabolites are an important focus of crop breeding and metabolic engineering research. However, our understanding of secondary metabolism is far from complete, particularly in non-model plants. Functional genomics, which includes transcriptomics, proteomics, and metabolomics, opens a new avenue for deciphering secondary metabolism. Here we review the applications of functional genomics in secondary metabolism, including the discovery of novel genes, the identification of gene function, and the detection of novel pathways of the metabolic network. Results from these studies will accelerate our understanding of elicitation mechanism of secondary metabolism and how plants interact with their environment, ultimately improving the production of secondary metabolites by means of metabolic engineering. This article is protected by copyright. All rights reserved
    No preview · Article · Sep 2014 · Engineering in Life Sciences
  • Liang Peng · Mei Ru · Kunru Wang · Bo Li · Yancui Wang · Pengguo Xia · Zongsuo Liang
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    ABSTRACT: Salvia miltiorrhiza is a significant source of bioactive compounds providing human health effects. Here, we surveyed root yield and the active constituents' divergences of second generation S. miltiorrhiza (SP2) responding to a spaceflight environment. High-performance liquid chromatography was conducted for the comprehensive constituents' characterizations of 28 SP2 lines (224 individuals) and the ground control (eight individuals). The results showed that the mean fresh and dry weight of roots ranged from 116 to 172 g and 25 to 119 g, respectively, in SP2 lines. In addition, the mean contents of four tanshinone compounds (tanshinone I, tanshinone IIA, cryptotanshinone, and dihydrotanshinone I) of 28 SP2 lines varied from 0.32 to 1.04 mg · g(-1), 0.47 to 2.39 mg · g(-1), 0.25 to 1.60 mg · g(-1), and 0.53 to 1.67 mg · g(-1), respectively. Except for salvianolic acid B, which varied drastically from 72 % to 201 % of the ground control treatment, the other six phenolic acid contents of the 28 SP2 lines all increased after spaceflight. Principal component analysis was performed to obtain an overview of the distribution of all samples, and score plots clearly separated the SP2 accessions from ground controls. Moreover, a positive relationship was observed between tanshinone I and tanshinone IIA (r = 0.790, p < 0.01), and rosmarinic acid was positively correlated with salvianolic acid B (r = 0.728, p < 0.01). In conclusion, this study demonstrated that a spaceflight environment induced SP2 accessions remarkably in the variation of root yield and active constituent content.
    No preview · Article · Aug 2014 · Planta Medica

Publication Stats

504 Citations
119.09 Total Impact Points


  • 2014-2015
    • Zhejiang Sci-Tech University
      Hang-hsien, Zhejiang Sheng, China
    • Northwest University
      Northwest Harborcreek, Pennsylvania, United States
  • 2007-2015
    • Northwest A & F University
      • College of Life Sciences
      Yang-ling-chen, Shaanxi, China
  • 2013
    • Hangzhou University
      Hang-hsien, Zhejiang Sheng, China
  • 2010-2013
    • Chinese Academy of Sciences
      • State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau
      Peping, Beijing, China
  • 2009
    • Northwest University
      • College of Life Sciences
      Xi’an, Shaanxi Sheng, China
  • 2005
    • Fujian Agriculture and Forestry University
      Min-hou, Fujian, China