Ching-Hui Yeh

Kaohsiung Armed Forces General Hospital, Kao-hsiung-shih, Kaohsiung, Taiwan

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Publications (4)5.75 Total impact

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    ABSTRACT: Background: Dendritic cells (DCs) are professional antigen-presenting cells and have critical roles in regulating immune responses. Prostaglandin I2 (PGI2) analogs are considered to be potential treatments for asthma. However, the effect of PGI2 analogs on human monocyte-derived DCs (MDDCs) is still not clearly understood. Methods: Human MDDCs were pretreated with iloprost and treprostinil (2 PGI2 analogs) or forskolin (an adenyl cyclase activator) before lipopolysaccharide (LPS) stimulation. In some cases, I prostanoid (IP) receptor and E prostanoid receptor antagonists were added before the PGI2 analog treatment. tumor necrosis factor α (TNF-α) was measured by enzyme-linked immunosorbent assay. The expression of costimulatory molecules was assessed by flow cytometry. T-cell polarization function was investigated by measuring interferon γ, interleukin 13 (IL-13), and IL-17A production by T cells cocultured with iloprost-treated MDDCs. Results: Iloprost and treprostinil suppressed LPS-induced TNF-α expression in MDDCs. This effect could be reversed by an IP receptor antagonist, CAY10449, but not by E prostanoid receptor antagonists. Forskolin conferred a similar effect. Iloprost suppressed the LPS-induced expression of costimulatory molecules, including CD80, CD86, CD40, and HLA-DR. Iloprost-treated MDDCs increased IL-17A production by T cells. Conclusions: Prostaglandin I2 analogs may exert anti-inflammatory effects by suppressing TNF-α expression via the IP receptor-cyclic adenosine monophosphate pathways and by inhibiting the expression of costimulatory molecules in human MDDCs.
    No preview · Article · Sep 2011 · Journal of Investigative Medicine
  • [Show abstract] [Hide abstract]
    ABSTRACT: Dendritic cells (DCs) are professional antigen-presenting cells and have critical roles in regulating immune responses. Prostaglandin I2 (PGI2) analogs are considered to be potential treatments for asthma. However, the effect of PGI2 analogs on human monocyte-derived DCs (MDDCs) is still not clearly understood. Human MDDCs were pretreated with iloprost and treprostinil (2 PGI2 analogs) or forskolin (an adenyl cyclase activator) before lipopolysaccharide (LPS) stimulation. In some cases, I prostanoid (IP) receptor and E prostanoid receptor antagonists were added before the PGI2 analog treatment. tumor necrosis factor α (TNF-α) was measured by enzyme-linked immunosorbent assay. The expression of costimulatory molecules was assessed by flow cytometry. T-cell polarization function was investigated by measuring interferon γ, interleukin 13 (IL-13), and IL-17A production by T cells cocultured with iloprost-treated MDDCs. Iloprost and treprostinil suppressed LPS-induced TNF-α expression in MDDCs. This effect could be reversed by an IP receptor antagonist, CAY10449, but not by E prostanoid receptor antagonists. Forskolin conferred a similar effect. Iloprost suppressed the LPS-induced expression of costimulatory molecules, including CD80, CD86, CD40, and HLA-DR. Iloprost-treated MDDCs increased IL-17A production by T cells. Prostaglandin I2 analogs may exert anti-inflammatory effects by suppressing TNF-α expression via the IP receptor-cyclic adenosine monophosphate pathways and by inhibiting the expression of costimulatory molecules in human MDDCs.
    No preview · Article · Jun 2011 · Journal of Investigative Medicine
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    Szu-Ming Wu · Kuo-Chen Wu · Hsiang-Chi Wu · Ching-Hui Yeh
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    ABSTRACT: Objectives: Sport participation always carries the risk of injury. The aim of this paper is to investigate the incidence of sports injury, the role of medical diagnostic devices and concepts of sports injury prevention. Various medical diagnostic devices are not always helpful without any damage. The conclusion of this study gives viewpoints of clinical medicine and biomedical engineering on sports injury. Highlighting the importance of sports injury with regard to incidence rates and risk factor is also the main goal of the study [1] . Results: The study population includes patients and their family members from the outpatient department of Tzuo-Ying Armed Forces General Hospital in Kaohsiung City, Taiwan. The data demonstrates the most common sites of injuries are ankles (34.4%). Regarding the knowledge of sports injury prevention, 38.3 % believe that sports protective devices using can decrease risk of injury and 51.5 % think that enough warm-up exercise will make exercise course smoothly. Regarding actions taken by persons after the occurrence of a sports injury, more than half (54.7%) of them attempt to treat the condition themselves at the initial stage, and only 33.6% look for medical attention immediately .It warns policy makers that the high rate of people ignore the sports injury and taking no thoughts about sports injury may result in more costs of the medical service at the later stage. Conclusions: The results show that increasing population levels of the knowledge about sports injury prevention and providing realistic medical diagnostic devices are important. [Life Science Journal 2010;7(2):98-101]. (ISSN: 1097-8135).
    Preview · Article · Jun 2010 · Life Science Journal
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    ABSTRACT: The expression of chemokines is critical in leukocyte recruitment and inflammation, but the regulatory mechanisms involved remain incompletely defined. While endocrine disrupter chemicals (EDCs) are known to be ubiquitous in the environment and often associated with altered inflammatory response, their potential impact on chemokine expression in monocytes is at present unknown. To this end, the effects of EDCs on the expression of Th1- and Th2-related chemokines in a human monocytic cell line, THP-1, were investigated. THP-1 cells were pre-treated with varying concentrations of EDCs (nonylphenol and 4-octylphenol) with or without the addition of an estrogen receptor (ER) antagonist, ICI 182,780 and then stimulated by lipopolysaccharide (LPS). The levels of chemokines, CXCL10/ IFN-alpha-inducible protein 10 (IP-10, a Th1 chemokine) and monocyte-derived chemokine (MDC)/CCL22, a Th2 chemokine) were measured by ELISA. EDC-mediated signaling events and histone modifications were examined by the use of Western blotting and chromatin immunoprecipitation (ChIP) assay. Nonylphenol and 4-octylphenol were able to suppress LPS-induced MDC and IP-10 expression. This suppressive effect was not reversed by the addition of ICI 182,780. Nonylphenol and 4-octylphenol reduced LPS-induced activation of MAPK signaling pathway, MKK1/2 and ERK, concomitant with decreased levels of LPS-induced acetylated histone 4 (H4) at the IP-10 and MDC gene loci. Nonylphenol and 4-octylphenol suppressed LPS-induced MDC expression in monocytes via, at least in part, the MKK1/2-ERK MAPK pathway and histone H4 acetylation, but not the estrogen receptor.
    No preview · Article · Sep 2009 · Inflammation