- [Show abstract] [Hide abstract] ABSTRACT: MADS-box genes encode a family of transcription factors that control a diverse range of processes in flowering plants. In this study, 13 unique MADS genes were cloned from Betula platyphylla Suk., and 2-year-old Betula seedlings propagated in glasshouses were selected as plant materials. The expression profile of each BpMADS was investigated during the growth season and following gibberellin (GA) treatments by real-time quantitative reverse transcription polymerase chain reaction. The relative abundance of the 13 BpMADS was shown to differ during each month, indicating that the activity of the genes varies during the annual growing period. Expression analyses demonstrated that these BpMADS were regulated by GA signaling pathways. Furthermore, the variations in expression patterns suggest that the genes act independently to fulfill specific functions or act cooperatively in physiological processes. The study of birch MADSs is important for the purposes of improving breeding techniques and molecular biotechnology. Keywords Betula platyphylla Suk.–MADS-box genes–Flowering–Gibberellins
- [Show abstract] [Hide abstract] ABSTRACT: The ThPOD1 gene encodes a peroxidase and was isolated from a Tamarix hispida NaCl-stress root cDNA library. We found that ThPOD1 expression could be induced by abiotic stresses such as cold, salt, drought and exogenous abscisic acid. These findings suggested that ThPOD1 might be involved in the plant response to environmental stresses and ABA treatment. To elucidate the function of this gene, recombinant plasmids expressing full-length ThPOD1 as well as ThPOD2 (aa 41-337), and ThPOD3 (aa 73-337) truncated polypeptides were constructed. SDS-PAGE and Western blot analyses of the fusion proteins revealed that the molecular weights of ThPOD1, ThPOD2 and ThPOD3 were approximately 57, approximately 50 and approximately 47 kDa, respectively. Stress assays of E. coli treated with the recombinant plasmids indicated that ThPOD3 could improve resistance to drought stress. This finding could potentially be used to improve plant tolerance to drought stress via gene transfer.
- [Show abstract] [Hide abstract] ABSTRACT: The ThCAP gene, which encodes a cold acclimation protein, was isolated from a Tamarix hispida NaCl-stress root cDNA library; its expression patterns were then assayed by qRT-PCR in different T. hispida tissues treated with low temperature (4 degrees C), salt (400 mM NaCl), drought (20% PEG6000) and exogenous abscisic acid (100 microM). Induction of ThCAP gene was not only responsive to different stress conditions but was also organ specific. When transgenic Populus (P. davidiana x P. bolleana) plants were generated, expressing ThCAP under regulation of the cauliflower mosaic virus CaMV 35S promoter, they had a greater resistance to low temperature than non-transgenic seedlings, suggesting that ThCAP might play an important role in cold tolerance.