Magda Sayed

National Research Center, Egypt, Al Qāhirah, Al Qāhirah, Egypt

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Publications (6)5.46 Total impact

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    ABSTRACT: Background: Progressive nephropathy represents a substantial source of morbidity and mortality in type 2 diabetes. Increasing albuminuria is a strong predictor of progressive renal dysfunction and heightened cardiovascular risk. Endothelial damage is associated with albuminuria. VonWillebrand factor (vWf) and Thrombomodulin (TM) are possible markers of endothelial dysfunction and damage. Objectives: We studied the relationship between plasma vWf, TM, and urinary albumin excretion. Methods: We conducted on 90 diabetic patients fulfilled the WHO criteria for type 2 diabetes. Diabetic patients were placed into three groups according to urinary albumin concentration (UAC) in a 24-hour urine collection. Group I: included 30 patients with normal urinary albumin concentration (without nephropathy); microalbumin/24 hour urine less than 30 mg. Group II: included 30 patients with microalbuminuria (incipient diabetic nephropathy), microalbumin/24 hour urine from 30 to 299 mg. Group III: included 30 patients with macroalbuminuria (overt diabetic nephropathy); microalbumin/24 hour urine greater than 300 mg. We measured plasma levels of vonWillebrand factor (vWf) and Thrombomodulin (TM) as markers of endothelial dysfunction, to evaluate their relationship to urinary albumin excretion in patients with type 2 diabetes mellitus. Results: There were significant increase in the levels of cholesterol and triglyceride, and significant decrease in HDL in group III compared to the control group, while it was highly significant with group II and III. TM levels showed no significant difference between control group and group I while it was highly significant with group II and III (p <0.01), also there was highly significant difference between group I and III (p <0.01). TM index showed no significant difference between control group and group I and II, while there was highly significant difference between control group and group III (p <0.01) and highly significant difference between group II and III (p <0.01). Albumin concentration in 24 hours urine showed no significant difference between control group and group I, while there were highly significant difference between control group and group II and III (p <0.01), also there was significant difference between group II and III (p <0.01). There were highly significant positive correlation between vWF, TM levels and all studied variables. Multivariate analysis which showed that total cholesterol, urinary albumin and age retained significant influence on the plasma vWF and TM. Conclusions: These results suggested that generalized vascular endothelial damage occurs in diabetic nephropathy including the microalbuminuric stage. Hence, plasma vWF and TM represents valuable markers of endothelial dysfunction that could be used for early detection of diabetic microvascular complications.
    No preview · Article · Apr 2012 · Asian biomedicine
  • Maha Hashim · Magda Sayed · Nervana Samy · Salah Elshazly
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    ABSTRACT: The prognosis of non small cell lung cancer (NSCLC) has remained disappointing over the last decades even in localized stages. Numerous prognostic factors have been investigated which might select patients for additional treatment. The objective of the current study was to assess the prognostic significance of telomerase activity, serum anti-p53 antibodies (anti-p53a), c-erbB-2 and CEA in patients with NSCLC. The study included 60 patients with histological proven NSCLC besides 60 controls (30 smokers and 30 nonsmokers). Patients were divided into four stages according to their histopathology. All patients were subjected to; determination of telomerase activity by telomeric repeat amplification protocol (TRAP) assay in tumor tissue specimens and adjacent normal lung tissues, also, determination of preoperative serum anti-p53a, c-erbB-2 and CEA. Telomerase activity was detected in 40 of 60 (66.6%) of NSCLC tissue specimens using the TRAP assay. As regard the stages, telomerase activity was positive in 5 of 15 patients (33.3%) with stage I NSCLC, in 11 of 20 patients (55%) with stage II NSCLC, in 9 of 10 patients (90%) with stage III NSCLC and in all patients (100%) with stage IV NSCLC. More cases of positive telomerase activity were observed in the group with advanced disease and in the group with poorly differentiated tumors. Telomerase activity was not detected in any normal lung tissue. The concentrations of serum anti-p53a, c-erbB-2, CEA were significantly higher in patients with NSCLC in comparison to the smoker and nonsmoker controls and their levels increased according to the stage of disease. Logistic regression test showed a relation between telomerase positivity and anti- p53a but no relation with c-erbB2, CEA. Telomerase activity was detected in most of NSCLC tissues; it was detected more frequently in advanced disease than early-stage disease. Anti-p53, c-erbB-2 and CEA were significantly higher in patients with NSCLC than controls and this increment was more evident in late stages of the disease. So, these biological markers might be useful predictors of prognosis. They may be helpful in defining groups of patients with NSCLC who could benefit from adjuvant treatments, also these markers can be used as therapeutic targets.
    No preview · Article · Feb 2010 · Medical Oncology
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    Mie Afify · Magda Sayed · Amr Elhammady
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    ABSTRACT: Biochemical markers are a non-invasive way of objectively measuring inflammation in ulcerative colitis and can play an adjunctive or primary role in the assessment of disease activity. Aim of this study was to A) evaluate serum levels of some biomarkers "leptin, adiponectin, resistin, and ghrelin" in ulcerative colitis (UC) patients, besides the ordinary inflammatory markers, B) to correlate the results with the disease activity, with the clinical characteristics of the disease C) and to examine the possible interaction between the estimated parameters values. Study was conducted on 56 UC patients from the Clinic of Internal Medicine Department and Endoscopy Unit of Alzahraa Hospital, Alazhar University, besides 30 healthy subjects served as control group. Results: Mean levels of ESR, CRP, TNF-α, resistin and ghrelin were significantly higher in active UC patients than the control group, while after the courses of treatment 47 patients achieved complete remission (inactive UC) mean values of these biochemical parameter deceased significantly than the original values at the active disease and the values reached nearly the normal ranges. While in patients (9 patients) who did not achieved complete remission, there were moderate decreased serum levels of these biochemical markers but still higher values than the control group and they still have manifestations of active UC. The mean level of leptin was significantly decreased in active UC patients compared to the control group, while after the course of treatment in patients achieved complete remission (inactive UC) the mean value increased significantly (with mean value 10.1 ng/ml). Conclusion: Our data indicate that, the increased plasma resistin, TNF-α and ghrelin levels correlated with activity of ulcerative colitis and so they could predict the response to therapy and possibly reflect an acute-phase response due to inflammation more than the ordinary inflammatory markers. Resistin, TNF-α and ghrelin levels could be considered as an independent predictor of disease activity in patients with UC and may represent link between inflammation and UC. [Journal of American Science 2010; 6(6):146-155]. (ISSN: 1545-1003).
    Full-text · Article · Jan 2010
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    Esmat Ashour · Nervana Samy · Magda Sayed · Azza Imam
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    ABSTRACT: The mechanisms underlying steatosis during hepatitis C virus (HCV) infection are complex and multifactorial. The aim of our study was to assess whether host metabolic factors influence the degree of hepatic steatosis and fibrosis in patients infected with hepatitis C virus genotype 4 by investigating the role of adiponectin, leptin and insulin resistance. Adiponectin and leptin levels, HCV genotypes, HCV-RNA, IR (HOMA-IR), body mass index and liver steatosis and fibrosis were assessed in 74 chronic patients with HCV genotype 4. Chronic HCV patients with steatosis showed lower serum adiponectin levels and higher levels of leptin, HOMA, alanine aminotransferase, gamma-glutamiltransferase and fibrosis scores. Low adiponectin levels were independently associated with grades of steatosis and HOMA-IR. Adiponectin levels showed significant inverse correlation between adiponectin and steatosis grade, BMI, HOMA and fibrosis stage. The multivariate analysis of factors showed that steatosis was significantly associated with low adiponectin concentration while leptin, insulin, HOMA, ALT, gamma-GT and cholesterol were positively associated with steatosis. This study stated that patients with HCV genotype-4 suffering from steatosis had a lower adiponectin level which is inversely correlated with insulin resistance. These data support a role for adiponectin in protecting against liver injury and also that hypoadiponectinaemia may contribute to the progression of hepatic steatosis. Further molecular and genetic studies with larger numbers of patients are required to confirm these results.
    Full-text · Article · Jan 2010 · Clinical laboratory
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    ABSTRACT: Obesity is a major risk factor for insulin resistance, type 2 diabetes, heart disease, and many other chronic diseases The current study was designed to investigate the endogenous mechanism by which obesity may increase the risk of CVD by examining whether serum adiponectin, Leptin or insulin mediate the association of obesity and type2 diabetes and cardiovascular risk factors in Egyptian adult patients. Patients and Methods: This study included 82 subjects, 30 patients suffering from type 2 diabetes and 52 patients suffering from type 2 diabetes together with coronary artery disease (CAD) together with another group having CAD without diabetes. They were classified according to their body mass index (BMI) into obese and non-obese groups, also 25 healthy volunteers were considered as controls. All patients were subjected to anthropometric assessment and laboratory determination of serum Adiponectin, Leptin, insulin and glucose. Insulin resistance was established by homeostasis model assessment (HOMA-IR) Differences in clinical or laboratory parameters among groups were compared by using one-way ANOVA test. Results revealed highly significant decrease in Adiponectin levels and highly significant increase in serum Leptin in non obese groups (G1 (T2D), G2 (CAD) and G3 (T2D+ CAD) as compared to controls. However, there were no statistical variations between non obese groups when compared to each others. HOMA-IR showed highly significant increase in non obese groups as compared to both controls and each other. Also, the results showed high significant decrease in Adiponectin and highly significant increase in Leptin in obese groups (G4 (T2D), G5 (CAD) and G6 (T2D+CAD) when compared to controls. However, there were no statistical variations between obese groups when compared to each others as regard Adiponectin, while Leptin showed statistical increase between (G4) and (G5) groups when compared to each others, HOMA-IR showed highly significant increase in the two obese groups only (G4 and G6) as compared to controls, while there was no significant variation in (G5) when compared to controls. Moreover, there was a significant increase in all obese groups when compared to each other. Also, there was significant correlation between serum Adiponectin and Leptin in obese DM patients. Conclusion: The coexistence of correlation between serum leptin and Adiponectin levels in addition to increase of serum leptin and decrease serum Adiponectin levels in obese DM patients in the current study; support the hypothesis of their susceptibility to atherosclerosis.
    No preview · Article · Jan 2010
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    Nervana Samy · Maha Hashim · Magda Sayed · Mohamed Said
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    ABSTRACT: Women with polycystic ovary syndrome (PCOS) have an increased prevalence of insulin resistance (IR) and related disorders. Elevated serum levels of high sensitivity CRP (hs-CRP), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-alpha) reflect low-grade chronic inflammation and have been associated with several insulin-resistant states; they are useful cardiovascular risk markers. The objective of this study was to investigate whether soluble inflammatory markers are altered in PCOS focusing on its relationship with obesity and indexes of insulin resistance. One hundred and eight women with PCOS and 75 healthy women were recruited. Patients were divided according to body mass index (BMI) into two groups; group I (BMI < 27 kg/m(2)) and group II (BMI > or = 27 Kg/m(2)). Serum levels of hs-CRP, IL-6, and TNF-alpha, lipid and hormone profiles were measured. PCOS patients had increased levels of testosterone, luteinizing hormone (LH), androstendione, insulin level and HOMA index compared to healthy BMI matched controls. High-density lipoprotein (HDL) concentrations were significantly reduced in both patient groups compared to their controls, while triglyceride levels were significantly increased in obese group compared to controls. There were no significant difference in serum inflammatory markers hs-CRP, IL-6 and TNF-alpha between group I and their matched controls. On the other hand, there were significant increase in these markers between group II and their matched controls. There were highly significant positive correlation between hs-CRP and IL-6 (r=0.702, P< 0.001) and between hs-CRP and TNF-alpha (r=0.621, P<0.001), also between IL-6 and TNF-alpha (r=0.543, P< 0.001). These inflammatory markers correlated significantly with BMI and HOMA index. Multiple regression analysis revealed that BMI and HOMA were predictors of IL-6 levels (b=11.173, P< 0.001, b=13.564, P< 0.001 respectively) and BMI was the only predictor of hs-CRP levels (b=12.578, P< 0.001) and TNF-alpha levels (b=0.134, P<0.001). PCOS and obesity induce an increase in serum inflammatory cardiovascular risk markers. The precise mechanisms underlying these associations require additional studies to clarify the state of the cardiovascular system in women with PCOS compared with controls in large numbers of patients to determine the relative contribution of different factors including insulin resistance, androgen status and BMI.
    Full-text · Article · Sep 2009 · Disease markers