Nazim Uddin Ahmed

Bangladesh Council of Scientific & Industrial Research, Mujib City, Dhaka, Bangladesh

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Publications (15)7.54 Total impact

  • Nazim Uddin · Nazim Uddin Ahmed · Atiar Rahman · Rasheda Akter · Rehana Akter
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    ABSTRACT: This study investigated the quantitative phytochemical contents, total phenolics, total flavonoids, total carotenoids, antioxidative capacity, tannin, proanthocyanidins, lycopene, chlorophyll a, and chlorophyll b of the Stephania japonica extract. Comprehensive antioxidative effects of the extract were also investigated. Quantitative assays were conducted through both spectrophotometric and high-performance liquid chromatographic methods. Antioxidative effects were measured through FeCl3 reducing power, metal chelating power, reducing power, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) scavenging activity, N, N-dimethyl-1,4-diaminobenzene free radical scavenging activity, superoxide radical scavenging activity, and nitric oxide scavenging effect. The contents of total phenolics, total flavonoids, total carotenoids, total antioxidative capacity, tannin, proanthocyanidins, lycopene, chlorophyll a, and chlorophyll b were found to be 47.32 ± 0.75 mg tannic acid equivalent, 61.41 ± 1.58 mg catechin equivalent, 63.29 ± 2.21 mg, 22.85 ± 0.70 mg ascorbic acid equivalent, 76.17 ± 0.97 mg tannic acid equivalent, 94.96 ± 4.49 mg catechin equivalent, 22.19 ± 0.79 μg, 22.19 ± 0.79 μg, 7.52 ± 1.24 mg, and 10.43 ± 2.11 mg, respectively, in 1 g of ethanol extract. A high concentration of epicatechin, p-coumaric acid, and rutin hydrate and moderate concentration of caffeic acid and quercetin was detected in the extract. The IC50 value for ferric reducing power assay, metal chelating assay, reducing power assay, ABTS scavenging assay, N, N-dimethyl-1,4-diaminobenzene scavenging assay, superoxide scavenging assay and nitric oxide (NO) assay were 465.06 ± 7.32 μmol ascorbic acid/g, 1656.52, 270.55, 457.27, 632.74, 217.5, and 464.00 μg/mL, respectively. No beta carotene was detected in the extract. The extract was demonstrated to be a very potential source of antioxidative metabolites.
    No preview · Article · Jul 2015 · International Journal of Food Properties
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    ABSTRACT: Background This study was subjected to investigate different pharmacological properties of ethanol extract of Solena amplexicaulis root. Results The extract contains flavonoid, alkaloid, saponin and steroid compounds. The extract exhibited excellent antioxidant activity in DPPH radical scavenging activity. The extract also showed potent activity in brine shrimp lethality bioassay. The LC50 value was found to 44.677 μg/ml. The extract showed better anti-bacterial activity against gram-negative bacteria. In antifungal assay, the maximum 79.31% of anti-mycotic activity was observed against Aspergillus ochraceus while minimum 44.2% against Rhizopus oryzae. MIC value ranged between 1500–3000 μg/ml. The extract was found moderately toxic with a 24-hr LD50 value of 81.47 mg/kg in Swiss albino mice. The degree of inhibition by the ethanolic extract of the root was found less than that of standard analgesic drug diclofenac sodium. The extract also showed moderate anti-inflammatory and antinociceptive activity and anti-diabetic property. Reducing power of the extract was comparable with standard ascorbic acid. Moderate in vitro thrombolytic activity, lipid peroxidation inhibition property, metal chelating ability and stress-protective activity was also observed. Conclusion Ethanol extract of Solena amplexicaulis root can be valuable for treatment of different diseases.
    Full-text · Article · Aug 2014 · Biological research
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    ABSTRACT: The present study was undertaken to evaluate the antidiabetic properties with phytochemical screening of the ethanol leaves extracts of two common indigenous medicinal plants of Bangladesh -Azadirachta indica (Neem) and Andrographis peniculata (Kalomegh) on experimental animal model. Hypoglycemic activity of the concentrated ethanolic (90%) extract of Azadirachta indica and Andrographis peniculata were studied compared with that of a reference antidiabetic drug glimeperide in both glucose loaded and alloxan induced diabetic rats. The acute toxicity of these two plants extract was also studied. Experimental results showed that ethanol leaves extract (1 gm/kg) of Azadirachta indica and Andrographis peniculata significantly (P<0.01) reduced the elevated blood glucose level by 36.91 % and 40.65 % respectively in glucose loaded rats and 30.20 % and 32.18 % respectively in alloxan induced diabetic rats compared to the respective diabetic control group. This study strongly suggested that ethanol leaves extracts of Azadirachta indica and Andrographis peniculata possess significant antidiabetic activity and could be a potential source for treatment of diabetes mellitus.
    Full-text · Article · Jan 2013
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    ABSTRACT: The present study was carried out to investigate the antidiabetic and antioxidant effect with phytochemical screening of ethanol extract of Saurauia roxburghii. The ethanol extract of Saurauia roxburghii was evaluated for its antidiabetic activities in alloxan induced diabetic rats. The significant reduction in blood glucose levels of the ethanol extract at 2g/kg dose exhibited a peak effect of 53.51% using glucometer. It gives the lowering blood glucose effects. The DPPH free radical scavenging effect of the ethanol extract was compared with standard antioxidant ascorbic acid. IC 50 values were found 37.844 µg/ml for the extract and 0.661 µg/ml for ascorbic acid. The ethanol extract was screened phytochemically for its chemical components. This revealed the presence of alkaloids, carbohydrates, cardiac glycosides, flavonoids, saponins, terpenes and steroids in moderate concentration. This study shows a possible beneficial effect of Saurauia roxburghii in the management of non-insulin dependent diabetes (NIDDM) and antioxidant activity. INTRODUCTION World Health Organisation (WHO) has defined medicinal plants as plants that contain properties or compounds that can be used for therapeutic purposes or those that synthesize metabolites to produce useful drugs [1] . The alkaloidal fractions of the leaf have been reported to possess weak activity against Kelebsiella pneumonia, Pseudomonas aeruginosa, Bacillis megaterium, Bacillus subtitis, Escherichia coli, Proteus mirabilis, Staphylococcus aureus, Aspergillus niger, Microsporum species and Penicillium species [2] . The alkaloidal fraction of the stem has also been reported to be active against all micro-organisms mention above [2] . The history of diabetes is millennium old. It writings from the earliest civilizations (Asia Minor, China, Egypt, and India) refer to boils and infections, excessive thirst, loss of weight and the passing of large quantities of honey sweet urine which often drew ants
    Full-text · Article · Oct 2012
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    ABSTRACT: To investigate the antilithiatic effect of the whole Leea macrophylla (L. macrophylla) Roxb (Leeaceae) ethanol extract in ethylene glycol-induced urolithiasis model of rats. Forty two seven weeks old male wistar albino rats were randomly divided into two major groups namely: preventive (n=18) and therapeutic (n=24). Preventive group was further subdivided into 3 groups of 6 rats namely: preventive control (PC), preventive lithiatic control (PLC) and preventive lithiatic L. macrophylla (PLLM). Similarly, therapeutic group was also subdivided into 4 groups of 6 rats namely: therapeutic control (TC), therapeutic lithiatic control (TLC), therapeutic lithitatic L. macrophylla (TLLM) and therapeutic lithiatic cystone (TLCYS). The lithiasis was induced by 0.75% (v/v) ethylene glycol in the drinking water of all groups except the PC and TC groups. The urinary ionic parameters such as calcium, inorganic phosphate, oxalate, magnesium & creatinine and renal morphology were altered by ethylene glycol, which were partially recovered by 14 d preventive and almost fully recovered by 28 d therapeutic intervention trials with L. macrophylla extract (500 mg/kg BW daily). Significant difference on recovery was observed between preventive and therapeutic interventional trials. Anti-urolithiatic effect of cystone was significantly (P<0.001) higher than extracts. L. macrophylla extract was found nontoxic in the acute toxicity test. The results of this study demonstrated very promising anti-urolithiatic effect of L. macrophylla extract with preventive and therapeutic treatments in this experimental condition.
    Full-text · Article · Jul 2012 · Asian Pacific Journal of Tropical Medicine
  • M. Ullah · Mir Showkat · Nazim Uddin Ahmed · Saiful Islam · Nurul Absar
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    ABSTRACT: Momordica charantia L. fruits, leaves, seeds and roots are considered as valuable traditional medicine. It is considered antidotal, antipyretic, tonic, antispasmodic, mild hypotensive. It builds immunity, relieves fever and reduce cholesterol level. To give a scientific basis for medicinal usage of this medicinal plant, the fruit extract was evaluated for the analgesic and anti-inflammatory activities. In this study, the acute toxicity study, analgesic and anti-inflammatory activities of Momordica charantia L. fruit extract were evaluated in a series of in vivo assay. Acetic acid induced writhing test and tail immersion test in mice were used to study the analgesic effect, while the effect of the extract on acute inflammation was investigated by carrageenan-induced paw edema in rats. For phytochemical analysis in vitro assay were performed according to the slandered procedure. The oral administration of M. charantia extract upto 2 g kg -1 in mice was found to be safe The extract significantly (p<0.001) inhibited acetic acid induced writhing and tail-immersion test induced pain at dose 500 mg kg -1, p.o. The extract also produced a moderate anti-inflammatory activity which was found to be significant at all the doses tested. The ethanolic extract showed 42.10% anti-inflammatory effect at dose 500 mg kg -1, p.o. Preliminary phytochemical screening of the extract revealed the presence of alkaloids, saponin, glycosides, steroids and sterol. The results obtained in this study lend credence to the ethnomedical use of the plant in the management of pain and inflammatory conditions. Thus, supporting the development of the biologically active substances as analgesics and anti-inflammatory agents. M. charantia may therefore, be a good candidate for functional foods as well as pharmaceutics.
    No preview · Article · Mar 2012 · Research Journal of Medicinal Plant
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    Md. Atiar Rahman · Md. Mizanur Rahman · Nazim uddin Ahmed
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    ABSTRACT: Essential oil of Alpinia calcarata Rosc. rhizome was studied for anti-inflammatory and anti-nociceptive effects in animal models. Anti-inflammatory effect was investigated in carrageenan-induced paw edema model in Albino rat with the doses of 100, 200 and 400 mg/kg BW. Anti-nociceptive effect was measured in acetic acid-induced pain, formalin-induced pain and thermal pain (hot plate test) models in Albino mice with the doses of 200, 400 and 600 mg/kg BW. Significant (pth min of treatment in hot plate model. Results evidenced that essential oil from Alpinia calcarata could be processed to use as an alternative source of inflammatory and nociceptive treatment.
    Full-text · Article · Jan 2012
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    ABSTRACT: Antibacterial effect of Crinum asiaticum bulb extract (1mg/disc) was tested on four Gram- positive and six Gram-negative bacteria by disc diffusion method using kanamycin (30 ìg/disc) as standard antibiotic disc. The bulb extract (250-1000mg/disc) showed significant zone of inhibition against all Gram-positive and Gram-negative bacteria ranging from 12-14 mm in diameter. Antioxidant potential of the same extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging method. The extract showed remarkable free radical scavenging effect (95.96%) providing the IC50 value of 5.62 for the bulb extract and 5.46 for ascorbic acid (standard antioxidant) at the concentration of 1000 ìg/ml. The bulb extract was found to be (LC50 value 94.06 μg/ml) in Brine-Shrimp lethality test.
    Full-text · Article · Jun 2011
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    ABSTRACT: The present study was carried out to investigate the antidiabetic and antioxidant effect with phytochemical screening of ethanol extract of Saurauia roxburghii. The ethanol extract of Saurauia roxburghii was evaluated for its antidiabetic activities in alloxan induced diabetic rats. The significant reduction in blood glucose levels of the ethanol extract at 2g/kg dose exhibited a peak effect of 53.51% using glucometer. It gives the lowering blood glucose effects. The DPPH free radical scavenging effect of the ethanol extract was compared with standard antioxidant ascorbic acid. IC 50 values were found 37.844 µg/ml for the extract and 0.661 µg/ml for ascorbic acid. The ethanol extract was screened phytochemically for its chemical components. This revealed the presence of alkaloids, carbohydrates, cardiac glycosides, flavonoids, saponins, terpenes and steroids in moderate concentration. This study shows a possible beneficial effect of Saurauia roxburghii in the management of non-insulin dependent diabetes (NIDDM) and antioxidant activity. INTRODUCTION World Health Organisation (WHO) has defined medicinal plants as plants that contain properties or compounds that can be used for therapeutic purposes or those that synthesize metabolites to produce useful drugs [1] . The alkaloidal fractions of the leaf have been reported to possess weak activity against Kelebsiella pneumonia, Pseudomonas aeruginosa, Bacillis megaterium, Bacillus subtitis, Escherichia coli, Proteus mirabilis, Staphylococcus aureus, Aspergillus niger, Microsporum species and Penicillium species [2] . The alkaloidal fraction of the stem has also been reported to be active against all micro-organisms mention above [2] . The history of diabetes is millennium old. It writings from the earliest civilizations (Asia Minor, China, Egypt, and India) refer to boils and infections, excessive thirst, loss of weight and the passing of large quantities of honey sweet urine which often drew ants
    Full-text · Dataset · Mar 2011
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    ABSTRACT: The hypocholesterolemic effects of the ethanol extract of Ananas comosas (L.) Merr. (Pineapple) was investigated in 5 groups of albino rats, 7 in each group. Group I rat received only distilled water and served as normal control. Groups II, III, IV and V were made hypercholesterolemic by feeding cholesterol orally suspended in 2% cholic acid mixed soybean oil (1 ml/kg bw) at a dose of 100 mg/kg bw daily up to 60 days. Group II rat received 1 ml distilled water while animals of Group III, IV and V received ethanol extract of Ananas comosas (L.) Merr. at the dose of 1000 mg/kg, 500 mg/kg and 100 mg/kg body weight respectively daily up to 60 days in addition to cholesterol as above. At the end of 60th day animals were sacrificed and blood samples were collected for measurement of lipid profiles using enzymatic kit. Administration of cholesterol caused a significant rise (p<0.001) in the serum levels of total cholesterol, LDL-cholesterol and triglycerides. Simultaneous administration of three different doses of Ananas comosas (L.) Merr. extract namely 1000 mg/kg, 500 mg/kg and 100 mg/kg for 60 days decreased serum cholesterol level by 62%, 47%, and 42% respectively (p<0.001); serum triglycerides level by 62.7%, 58.7%, and 49.09% respectively (p<0.001); serum LDL level by 70.5%, 53.9% and 51.15%, respectively (p<0.01). However, serum HDL level was not affected significantly. The cumulative results clearly indicate ethanol extract of Ananas comosas (L.) Merr. leaves possesses potent hypocholesterolaemic effect.
    Full-text · Article · Jan 2011
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    ABSTRACT: Clausena suffruticosa (Family: Rutaceae) root ethanolic extract was subjected to analyze for its antioxidant effect by DPPH free radical scavenging method, antibacterial & antifungal effect by disc diffusion technique and cytotoxic effect by brine shrimp lethality test. The extract showed very significant antioxidant activity with the LC 50 value of 3.28µg/ml. In antibacterial screening, moderate zone of inhibition (7.5-9.0mm in diameter) was observed against gram positive Bacillus subtilis, Bacillus cereus, Streptococcus aureus, Bacillus polymyxa & Bacillus megaterium and promising zone of inhibition (10.0-13.0mm in diameter) against gram negative Salmonella typhi, Shigella flexneri,, Proteus sp. & Escherichia coli. Klebsiella sp. and Shigella sonnei did not show sensitivity. The MIC values against these bacteria were ranged from 3,000 to 4,500µg/ml. Root extract showed significant zone of inhibition against Aspergillus ustus, Aspergillus niger and Aspergillus ochraceus in antifungal assay. In the cytotoxicity assay, LD 50 and Chi-square value of the ethanolic extract against brine shrimp nauplii were 546.94µg/ml and 1.9932 demonstrating potent cytotoxic effect of the extract.
    Full-text · Article · Jan 2011 · Journal of Applied Pharmaceutical Science
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    ABSTRACT: The bark of Adenanthera pavonina L. was extracted in pet-ether (PE), dichloromethane (DCM), ethyl acetate (EA) and methanol (ME) successively and evaluated the analgesic, antimicrobial, antioxidant activity of the extracts. The phytochemical screening of the extracts was carried out using standard method of analysis. This investigation revealed saponins, alkaloids, carbohydrate, cardiac glycosides, tannins, flavanoids and steroids are rich in ME and EA extracts. In the analgesic activity by acetic acid-induced writhing test in mice, the extracts were given interperitonially at a dose of 100 and 200 mg/kg in different groups of mice. The results showed that DCM extract (200 mg/kg) showed the highest (72.081%) analgesic activity while PE extracts (100mg/kg) showed the lowest (23.35%) activity in comparison to those of indomethacin (8 mg/kg) and diclofenac sodium (15 mg/kg) as reference drugs. The highest antimicrobial potential was observed in crude ME extracts against gram-positive, gram-negative bacteria and fungi at dosage levels of 100, 200, and 400 μg/disc. Their zones of inhibition were compared with the standard antibiotic Kanamycin (30 μg/disc). The extracts exhibited significant free radical scavenging activity when compared with ascorbic acid (ASA) and tert-butyl-1-hydroxytoluene (BHT). The IC50 value for antioxidant activity of PE, DCM, EA and ME extracts were found to be 390.33±2.78, 32.13±0.38, 8.72±0.11 and 6.44±0.04μg/ml respectively. The results suggest that the extract could be used in the treatment of pain disorder, as anti-microbial as well as anti-oxidant.
    Full-text · Article · Sep 2010 · Advances in Natural and Applied Sciences
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    ABSTRACT: This study was subjected to investigate antioxidant, cytotoxic and antimicrobial (antibacterial and antifungal) properties of Eclipta alba ethanol extract. The antioxidant and cytotoxic properties of the extract were assessed by DPPH free radical scavenging method and brine shrimp lethality bioassay, respectively. Disc diffusion technique and food poisoning technique were used to determine the antibacterial and antifungal activity of the extract, respectively. DPPH free radical scavenging effect of extract was compared with standard antioxidant ascorbic acid. IC50 value was found 1.34μg/ml for extract and 1.03μg/ml for ascorbic acid. LC50 value of the extract in brine shrimp lethality bioassay was found 94.3μg/ml. Large zone of inhibition were observed in disc diffusion antibacterial screening against gram negative Salmonella typhi, Shigella sonnei, Escherichia coli, Salmonella paratyphi, Pseudomonas sp (1) & Pseudomonas sp (II), and gram positive Bacillus subtilis, Bacillus cereus, Bacillus megaterim & Staphylococcus aureus at the concentration of 1mg/disc. The extract concentration 250μg/disc showed no zone of inhibition to any bacterial strain but 500μg/disc showed a moderate zone of inhibition (8mm) against Salmonella typhi. In antifungal assay, the maximum 51.52% of anti-mycotic activity was observed against Aspergillus ochraceus. Fluconazole was used as standard antifungal agent. Extract was found nontoxic in acute toxicity test in mice.
    Full-text · Article · Nov 2009
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    Full-text · Article · Nov 2009 · Bangladesh Journal of Scientific and Industrial Research
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    ABSTRACT: The ethanolic bark extract of Mitragyna diversifolia showed significant (p<0.05) antidiarrheal activity on gastrointestinal motility with barium sulfate milk model and castor oil- induced diarrheal model in rats. These results revealed that the bark extract possess pharmacological activity against diarrhea and may possibly explain the use of the plant in traditional medicine.
    Full-text · Article · Apr 2009 · Bangladesh Journal of Pharmacology