[Show abstract][Hide abstract] ABSTRACT: Aims: This study aims to determine prevalence of Salmonella in shellfish and to study their resistance to antibiotics. Samples: Three species of shellfish consisted of cockles, clams and mussels were sampled monthly in six sites during two years 2008 and 2012. Methodology: As many as 272 samples of shellfish were examined for presence of Salmonella. Positives strains were confirmed for presence of invA gene, serotyping and tested for drug susceptibility. Results: Up to 7.7% of samples were positive for Salmonella and a total of 90 Salmonella isolates belonging to 4 serovars (S. Kentucky, S. Glostrup, S. Newport and S. Reading) were tested for their susceptibility to a panel of 12 antimicrobial agents. Many resistant isolates were detected with Original Research Article Boutaib et al.; BMRJ, 8(6): 663-669, 2015; Article no.BMRJ.2015.160 664 75.8% of isolates resistant to at least one antimicrobial agent. Isolates demonstrated resistant to streptomicin, chloramphenicol, nalidixic acid, ciprofloxacin, amoxicillin, respectively with (90.6%; 51.6%; 31.6%; 30.5% and 19%). The most common pattern of multiple drug resistance included resistance to chloramphenicol, ciprofloxacin, nalidixic acid. Conclusion: It seemed that strains isolated of Salmonella were multidrug resistant and almost one third of strains were resistant to quinolone. The results emphasize the need of a monitoring programme of bacterial pathogenes Salmonella on shellfish to protect human health.
[Show abstract][Hide abstract] ABSTRACT: A study was conducted to estimate the prevalence, antibiotic sensitivity and distribution of serotypes and virulence genes of Salmonella in surface waters of the Khoumane River. The serotypes as well as antibiotic-resistance patterns and gene virulence of the Salmonella isolates were determined. 84 water samples were tested during the August 2010- July 2011 period. Among them, 2, 38 % were positive for Salmonella. All strains were identified Salmonella Butantan. Results of antibiotic susceptibility tests showed that both Salmonella are sensitive to C3G and Quinolones and are resistant to Amoxicillin and Sulfonamides. All Salmonella strains tested were positive for the invasion gene invA and the virulence gene spvC carried by a plasmid with 54Kb size. These Salmonella can be transmitted to human populations through the use of water from the river.
No preview · Article · Jan 2014 · Journal of Materials and Environmental Science
[Show abstract][Hide abstract] ABSTRACT: A study was conducted to estimate the prevalence, antibiotic resistance and the distribution of serovars and virulence genes of Salmonella in raw turkey minced meat at Casablanca, Morocco. 192 samples were collected during the microbiological assessment of food between 2005-2008. Of the total 192 samples examined, Salmonella was detected in 39 (20,31%) of the samples analysed. Out of the total 39 Salmonella isolates, 15 different serotypes were identified of which S. Kentucky (20,5%) was the most frequent followed by, 5. Corvallis (15,3%), S. Muenster (12,8%), S. Newport (12,8%), S. Typhimurium (5,1%) and 1% for all the other serovars isolated. Results of the present study indicated that thirty two of 39 (82%) strains were resistant to one or more of the antibiotics employed and multi-drug-resistant strains constituted 51,3% of all isolates. Nine of these isolates were resistant to ciprofloxacin, which is the treatment of choice of severe non typhoidal Salmonella infections in adults. Examination by PCR revealed presence of the virulence gene invA in all serovars, and the spvC, h-li gene were found respectively in 2,6% and 18%. Results of the present study indicated that there was a high level of Salmonella contamination of raw turkey minced meat, which could be considered as one of the major potential source of human salmonellosis in Morocco.
[Show abstract][Hide abstract] ABSTRACT: The present paper describes a microbiological method for the detection of antibacterial substances in poulry muscle. The method is based on the growth inhibition of Bacillus subtilis on an agar medium at a pH of 6.0, 7.2, and 8.0, Bacillus cereus on an agar medium at a pH of 6.0, M. luteus on agar medium at a pH of 6.0 and 8.0, and Escherichia coli on agar medium at a pH of 7.2, and on the use of confirmatory solutions (Pase, Paba, MgSO4) for the identification. The proposed method detects the main antibiotic groups: β-lactams, tetracyclines, aminoglycosides, macrolides, and quinolones at low levels. The results are obtained in 18-24 h. This technique can be used as a screening method for the detection of antibiotics in animal tissue, but a more specific method would be necessary for full identification of antimicrobials in screening positive samples.
[Show abstract][Hide abstract] ABSTRACT: In this study, samples of raw ground beef (n = 150) and fresh sausage (n = 100) were collected randomly from butcheries, supermarkets, and fast-food shops, in Casablanca, Morocco. Two types of meat product samples were considered, one with spices (n = 115) and other without spices (n = 135). All the samples were analyzed for the presence of the following bacteria: Escherichia coli, Staphylococcus, Clostridium perfringens, Salmonella, and Listeria monocytogenes. E. coli strains were further typed by pulsed-field gel electrophoresis (PFGE), Operon O, and characterized for virulence genes by polymerase chain reaction (PCR). Results indicated that counts of E. coli, coagulase-positive Staphylococcus, and C. perfringens were 17%, 9.6%, and 8.7% in samples without spices, respectively; and 23.5%, 23.7%, and 29.6% in samples with spices, respectively. Two pathogenic genes, LT and EAST, were identified separately in four strains of E. coli. Salmonella and L. monocytogenes were isolated in 2.8% and 3.2% of the total samples, respectively.
No preview · Article · Dec 2008 · Journal of environmental health