W Grzeszczak

Medical University of Silesia in Katowice, Catowice, Silesian Voivodeship, Poland

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Publications (389)702.84 Total impact


  • No preview · Article · Oct 2015 · Endokrynologia Polska
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    ABSTRACT: In 770 postmenopausal women, the fracture incidence during a 4-year follow-up was analyzed in relation to the fracture probability (FRAX risk assessment tool) and risk (Garvan risk calculator) predicted at baseline. Incident fractures occurred in 62 subjects with a higher prevalence in high-risk subgroups. Prior fracture, rheumatoid arthritis, femoral neck T-score and falls increased independent of fracture incidence. The aim of the study was to analyze the incidence of fractures during a 4-year follow-up in relation to the baseline fracture probability and risk. Enrolled in the study were 770 postmenopausal women with a mean age of 65.7 ± 7.3 years. Bone mineral density (BMD) at the proximal femur, clinical data, and fracture probability using the FRAX tool and risk using the Garvan calculator were determined. Each subject was asked yearly by phone call about the incidence of fracture during the follow-up period. Of the 770 women, 62 had a fracture during follow-up, and 46 had a major fracture. At baseline, BMD was significantly lower, and fracture probability and fracture risk were significantly higher in women who had a fracture. Among women with a major fracture, the percentage with a high baseline fracture probability (>10 %) was significantly higher than among those without a fracture (p < 0.01). Fracture incidence during follow-up was significantly higher among women with a high baseline fracture probability (12.7 % vs. 5.2 %) and a high fracture risk (9.2 vs. 5.3 %) so that the "fracture-free survival" curves were significantly different (p < 0.05). The number of clinical risk factors noted at baseline was significantly associated with fracture incidence (chi-squared = 20.82, p < 0.01). Prior fracture, rheumatoid arthritis, and femoral neck T-score were identified as significant risk factors for major fractures (for any fractures, the influence of falls was also significant). During follow-up, fracture incidence was predicted by baseline fracture probability (FRAX risk assessment tool) and risk (Garvan risk calculator). A number of clinical risk factors and a prior fracture, rheumatoid arthritis, femoral neck T-score, and falls increased independent of fracture incidence.
    Preview · Article · Jul 2015 · Osteoporosis International
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    ABSTRACT: Transplant recipients have a significantly greater incidence of cancer, compared with the general population, who are referred to immunosuppressive therapy as an additional malignancy risk factor. Therefore, there is a need to search for an easy in clinical practice neoplasm predictor, especially for this group of patients. A group of 74 (43M and 31F; aged 46.8 ± 12 years) kidney transplant recipients was investigated in a three-year follow-up study. During the time of observation, 7 patients were diagnosed with neoplasm (7.4 ± 1.5 years after transplantation). A serum level of IL2 (ELISA test) and mRNA level of IL1beta, IL10 and TNFalfa in peripheral mononuclear blood cells - PBMCs (QRT - PCR method) were measured in every year of observation. Analysis of variances and t-Student test were used in groups mean comparison: N - patients developing malignant neoplasm group (24 probes); M - set of probes from patients with malignancies at the moment of diagnosis (11 probes); P - set of probes from patients before developing malignant neoplasm (10 probes); C - control group of healthy transplant recipients (31 probes). Among the analyzed agents, only serum IL2 level differed between the analyzed groups, with higher values in the M compared with the P group (p<0.05) and with C group (p<0.01). There were no differences neither between N and C or P and C groups (p = 0.98), nor any correlation between IL2 and IL1b, IL2 and TNFalfa. The results may indicate that IL2 serum level might be consider as a useful late unspecific cancer marker, although larger studies should yield verification of this finding.
    Full-text · Article · May 2015 · Annals of agricultural and environmental medicine: AAEM
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    Full-text · Dataset · Feb 2015
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    Full-text · Dataset · Feb 2015
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    ABSTRACT: The aim of the study was to investigate whether rs1800471 polymorphism in TGFB1 gene is associated with the development and progression of non-diabetic chronic kidney disease. Moreover, we examined the serum TGF-beta1 concentration and its association with that polymorphism and progression of the disease. We applied two different methodological approaches. Firstly, a family based study was carried out, comprised of 109 patients with non-diabetic chronic kidney disease and their 218 healthy parents, using the transmission/disequilibrium test. The rs1800471 polymorphism and serum TGF-beta1 level were determined in all subjects. Serum TGF-beta1 concentration was also measured in 40 healthy controls. Secondly, we performed a case-control orientated study to determine whether rs1800471 polymorphism and other factors influence the progression of renal impairment. We found no relationships between rs1800471 polymorphism allele transfer and the incidence or progression of non-diabetic chronic kidney disease. We found, however, that the serum TGF-beta1 was significantly higher in patients than in controls. In conclusion, rs1800471 polymorphism in TGFB1 gene does not have an impact on the development and progression of non-diabetic chronic kidney disease caused by primary glomerulopathy and chronic interstitial nephritis. The increased serum TGF-beta1 concentration in such patients suggests its role in the pathomechanism of the disease. Circulating TGF-beta1 level is determined in a multifactorial way, not by rs1800471 polymorphism in TGFB1 gene.
    No preview · Article · Oct 2014 · Advances in Experimental Medicine and Biology
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    ABSTRACT: Background: Renal ischemia-reperfusion injury (IRI) induces inflammatory reaction damaging kidney. Pentoxifylline (PTX) given before IRI attenuates inflammation and prevents ischemic acute kidney injury (iAKI). Given that in clinical settings IRI is not always predictable, we aimed to assess whether PTX administration during or shortly after IRI affects the course of iAKI in the rat. Methods: In 58 male 10-week-old Sprague-Dawley rats, 14 days after right nephrectomy, a 45-minute clamping of solitary renal pedicle was conducted. PTX 100 mg/kg body weight or 0.9% NaCl 1 mL were given subcutaneously either 60 minutes before renal ischemia, 1 minute into ischemia, or 60 minutes after clamp release. Creatinine clearance (ClCr; mL/min/kg body weight), fractional excretions of sodium (FENa [%]) and potassium (FEK [%]), and urine protein/ClCr ratio (Uprot/ClCr [mg/1 mL ClCr]) at 48 hours after IRI were compared between PTX-treated animals and respective controls (Mann-Whitney U test). Results: Kidney function was improved in rats given PTX before IRI compared with controls: ClCr 2.10 ± 0.44 versus 1.03 ± 0.18; FENa 0.16 ± 0.12 versus 0.84 ± 0.55; FEK 40.3 ± 13.0 versus 75.5 ± 17.9, respectively (all P < .001). There was no difference in proteinuria: Uprot/ClCr 0.004 ± 0.002 versus 0.004 ± 0.002. Conversely, the analyzed parameters did not differ between animals administered PTX during IRI and controls: ClCr 0.42 ± 0.34 versus 0.73 ± 0.43; FENa 2.98 ± 2.71 versus 3.16 ± 3.05; FEK 280.1 ± 155.7 versus 206.2 ± 154.1; and Uprot/ClCr 0.031 ± 0.029 versus 0.029 ± 0.031, respectively, nor between rats given PTX after IRI and controls: ClCr 0.29 ± 0.38 versus 0.40 ± 0.47; FENa 4.25 ± 3.55 versus 3.80 ± 3.94; FEK 284.9 ± 117.5 versus 243.0 ± 150.6; and Uprot/ClCr 0.044 ± 0.018 versus 0.055 ± 0.061, respectively. Conclusions: PTX given only before, and not at the time of renal ischemia or after reperfusion, alleviates subsequent iAKI in the rat. This implicates usefulness of PTX in the clinical settings of expected renal ischemia, like kidney transplantation, and suggests potential benefits of PTX in peritransplant period foremost with donor pretreatment.
    Full-text · Article · Oct 2014 · Transplantation Proceedings
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    ABSTRACT: Introduction: It is assumed that genetic factors may play a significant role in CKD development. The aim of the study was to investigate the role of rs7903146 polymorphism in the TCF7L2 gene in development and progression of non-diabetic chronic kidney disease (CKD). Material/methods: 109 children and young adults with CKD caused by primary glomerulopathy and tubulointerstitial nephropathy, stages 3-5, and their 218 biological parents with no renal dysfunction were included in the study. We tested the transmission of alleles of rs7903146 polymorphism in the TCF7L2 gene from heterozygous parents to offspring affected with CKD using the transmission/disequilibrium test. We also analysed whether rs7903146 polymorphism had any impact on the loss of glomerular filtration rate. Results: The rs7903146 polymorphism in TCF7L2 allele transmission from heterozygous parents to their affected children was not different from a random proportion expected for no association, in the whole group of subjects, and in the subgroups, depending on CKD aetiology. Lack of association between the analysed polymorphism and the loss of glomerular filtration rate was found in the total group of patients as well as in the subgroups, regarding the cause of CKD. Conclusions: This study found no association between rs7903146 polymorphism in the TCF7L2 gene and the increased risk for development of CKD caused by primary glomerulopathy and analysed tubulointerstitial nephropathy. The progression rate of CKD of non-diabetic aetiology does not depend on this polymorphism.
    No preview · Article · May 2014 · Postępy Higieny i Medycyny Doświadczalnej (Advances in Hygiene and Experimental Medicine)
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    ABSTRACT: Introduction and Aims: Angiotensinogen (Agt) is the sole precursor of all angiotensins. We have previously reported that high glucose stimulates renal Agt gene expression via nuclear factor erythroid 2-related factor 2 (Nrf2) in type 1 diabetic Akita mice. The present studies hypothesized that insulin inhibits renal angiotensinogen (Agt) gene expression via down-regulation of Nrf2 and thus prevents systemic hypertension in type 1 diabetic Akita mice and in wild type (WT) mice infused with insulin and various concentration of D-glucose to maintain normal blood glucose levels. Methods: Male Akita mice were studied from 10-16 weeks of age with or without insulin implants (Linβit) from week 12. Age- and sex-matched non-Akita littermates served as wild type (WT) controls. We also did insulin clamp studies in WT mice (age: 8-10 weeks) using insulin infusion (~3 ng/min/kg) plus variable concentrations of D-glucose to maintain euglycemia for 3 hours (hyperinsulinemia-euglycemic clamp). WT mice infused with saline served as controls. Kidneys were analyzed for Nrf2, Agt, and heterogeneous nuclear ribonucleoprotein F and K (hnRNP F/K) expression by immunohistochemistry. mRNA and protein expression in renal proximal tubules (RPTs) were evaluated by real time-qPCR and Western blotting, respectively. Immortalized rat renal proximal tubular cells (IRPTCs), stably transfected with plasmid pGL4.20 and containing either rat Nrf2, Agt, hnRNP F or hnRNP K gene promoter, were also studied in vitro. Results: In Akita mice, insulin treatment normalized blood glucose levels, reversed systemic hypertension and renal oxidative stress, inhibited renal Nrf2 and Agt gene expression, whereas it enhanced hnRNP F/K gene expression and attenuated renal hypertrophy, glomerular hyperfiltration and tubulointerstitial fibrosis. In WT mice subjected to euglycemic insulin clamp, renal Agt and Nrf2 gene expression was down-regulated, whereas hnRNP F/K gene expression was up-regulated as compared to saline-infused control WT mice. In vitro, insulin inhibited Nrf2 and Agt gene promoter activity, and stimulated hnRNP F/K gene promoter activity in high glucose media via the p44/42 mitogen-activated protein kinase signaling pathway. Transfection with Nrf2 small interfering RNA enhanced insulin inhibition of Agt and stimulated hnRNP F/K gene promoter activity in IRPTCs. Conclusions: Our data indicate that insulin prevents hypertension and attenuates kidney injury, at least in part, through suppressing renal Nrf2 and Agt gene transcription and up-regulating hnRNP F/K gene expression in diabetic Akita mice, independent of its glucose lowering effect.
    Full-text · Article · May 2014 · Nephrology Dialysis Transplantation
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    ABSTRACT: Introduction and objective: We wished to establish the prevalence of eye diseases and eye disease risk factors at postmenopausal age and to compare ophthalmic problems in urban and rural areas of Raciborz. Patients and methods: The study was performed in 2010. Out of the whole population of Raciborz, Poland, 10 percent (1750) of women were randomly selected for the reported study. Finally, ocular diseases, ophthalmic agents, health status (physical activity level, body mass index - BMI, reproductive history, the use of psychotropic drugs and hormone replacement therapy - HRT) were recorded in 623 women. The women underwent visual acuity test and anterior segment examination, applanation tonometry and indirect ophthalmoscopy. Results: The mean age of the selected patients was 66.01 ± 7.76 years, 275 (44%) of them originating from rural and 348 (56%) from urban regions. The average woman was obese (BMI=30.54 ± 5.38 kg/m(2)), with near normal agility and reproductive history of 2.59 ± 1.55 births, 147 (24%) subjects remained under regular HRT support. According to the WHO, the visual acuity was classified as normal or near normal in 87.5%, while no blindness was recorded at all. Visual acuity depended, first of all, on lens status and was better among subjects with good agility (R=-0.31, p=0.001). Dry eye prevalence increased significantly over age of 67 years (p=0.000) and HRT seemed to be a dry eye protective factor (p=0.010). Except age, No other risk factors of cataract, other than age, were identified. Normal agility (p=0.003) and HRT (p=0.032) were associated with lower AMD (age-related macular degeneration) prevalence rates. The differences between urban and rural participants were presented only in education, reproductive history, hypertension and frequency of ophthalmic examinations. Conclusions: Older adult women living in neighboring urban and rural areas present no differential in ophthalmic health problems.
    No preview · Article · Mar 2014 · Annals of agricultural and environmental medicine: AAEM
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    ABSTRACT: OBJECTIVE: The incidence of osteoporosis increases with age and is most frequently observed in postmenopausal women. The objective of the present population-based cohort study was to assess the influence of Ca intake from dairy sources on hip bone mineral density and hip fracture incidence in a group of Polish women over 55 years of age. DESIGN: The main outcome measures included: bone mineral density, the number of previous fractures and the reported Ca intake from dairy sources, assessed by a diet questionnaire. SETTING: The RAC-OST-POL Study was conducted in the District of Raciborz in the south of Poland. SUBJECTS: The study was carried out in a group of 625 women, randomly recruited from the general population of women aged >55 years. RESULTS: Median Ca intake from dairy products was lower in the group of women with femoral neck T-score ≤-2·5 than in the group with T-score >-2·5 (275 v. 383 mg/d; P = 0·0019). For total hip score, the difference was close to borderline significance (P = 0·0698). Median Ca intake from dairy products was lower in the group of women with previous fractures than in those without fracture history (336 v. 395 mg/d; P = 0·0254). The main dairy source of Ca in the analysed group included milk drinks, rennet cheese and milk. CONCLUSIONS: Higher dairy Ca intake is recommended, since a number of the women analysed were unable to satisfy their Ca requirement exclusively from their diet.
    Full-text · Article · Feb 2014 · Public Health Nutrition
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    ABSTRACT: The impact of fast changes in obesity indices on other measures of metabolic health is poorly defined in the general population. Using the Polish accession to the European Union as a model of political and social transformation we examined how an expected rapid increase in body mass index (BMI) and waist circumference relates to changes in lipid profile, both at the population and personal level. Through primary care centres in 444 Polish cities, two cross-sectional nationwide population-based surveys (LIPIDOGRAM 2004 and LIPIDOGRAM 2006) examined 15,404 and 15,453 adult individuals in 2004 and 2006, respectively. A separate prospective sample of 1,840 individuals recruited in 2004 had a follow-up in 2006 (LIPIDOGRAM PLUS). Two years after Polish accession to European Union, mean population BMI and waist circumference increased by 0.6% and 0.9%, respectively. This tracked with a 7.6% drop in HDL-cholesterol and a 2.1% increase in triglycerides (all p<0.001) nationwide. The direction and magnitude of the population changes were replicated at the personal level in LIPIDOGRAM PLUS (0.7%, 0.3%, 8.6% and 1.8%, respectively). However, increases in BMI and waist circumference were both only weakly associated with HDL-cholesterol and triglycerides changes prospectively. The relation of BMI to the magnitude of change in both lipid fractions was comparable to that of waist circumference. Moderate changes in obesity measures tracked with a significant deterioration in measures of pro-atherogenic dyslipidaemia at both personal and population level. These associations were predominantly driven by factors not measureable directly through either BMI or waist circumference.
    Full-text · Article · Jan 2014 · PLoS ONE
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    ABSTRACT: Lipodystrophies are a heterogeneous group of diseases affecting adipose tissue distribution. Familial partial lipodystrophy of the Dunnigantype (FPLD) is a rare autosomal, dominant disorder caused by missense mutations in lamin A/C (LMNA) gene where selectiveloss of subcutaneous adipose tissue from the limbs and trunk, and accumulation of fat in the neck and face, is usually associated witha variety of metabolic disorders including insulin resistance, diabetes mellitus, dyslipidemia, hepatic steatosis and high blood pressure.In this report we present clinical and molecular features of three Polish women with FLPD phenotype coming from one family (a motherand her two daughters). FPLD was recognised under the circumstances of diabetes treatment, where sequencing of LMNA gene revealedheterozygous R482W mutation. In order to be able to recognise monogenic diabetes associated with lipodystrophy, it is important to bevery precise in physical examination while diagnosing diabetes and to be aware of the necessity of performing genetic testing. Diabetesappropriatedifferential diagnosis is essential for the treatment strategy, anticipation of the disease progression, and determination of theprognosis. It is necessary for an individual mutation carrier to look carefully at the patient's family.
    Preview · Article · Sep 2013 · Endokrynologia Polska
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    ABSTRACT: Background Uremic pruritus is a common complication in patients undergoing dialysis. The pathophysiological mechanisms of pruritus in patients with end-stage renal disease remain unknown. Neuropeptides, including substance P, are postulated to play an important role in the pathogenesis of pruritus. The aim of this study was to evaluate the role of substance P in uremic pruritus in patients on hemodialysis and peritoneal dialysis. Material/Methods We included 197 patients with end-stage renal disease: 54 on continuous ambulatory peritoneal dialysis and 143 on hemodialysis. Substance P, calcium, phosphorus, iron, ferritin, CRP, albumin, hemoglobin, Ca × P product, and iPTH level were determined in all participants. The correlation between these parameters and self-reported itching was evaluated in patients on hemodialysis in comparison with peritoneal dialysis patients. Results The incidence of itching was similar in hemodialysis and peritoneal dialysis patients. No differences in substance P level between the 2 groups were found. There was no correlation between substance P level and the incidence or intensity of pruritus in dialyzed patients. Conclusions This study demonstrates that substance P does not play any important role in pruritus in hemodialysed and peritoneal dialyzed patients. However, further studies are necessary to assess the exact role of neuropeptides in uremic pruritus.
    Full-text · Article · Sep 2013 · Medical science monitor: international medical journal of experimental and clinical research

  • No preview · Article · Aug 2013 · European Heart Journal
  • Z Brzoza · W Grzeszczak · B Rogala · W Trautsolt · D Moczulski
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    ABSTRACT: Background: Autoimmune mechanisms play a role in the pathophysiology of chronic urticaria. As the genetic background of autoimmunity is well proven, the role of genetics in chronic urticaria is hypothesised. Methods: 153 unrelated chronic spontaneous urticaria patients with a positive result of autologous serum skin test were included into the study, as were 115 healthy volunteers as control group. In all subjects we analysed CCR2 G190A and CCR5 d32 polymorphisms. Results: We noticed higher prevalence of CCR2 A allele as well as lower frequency of CCR5 d32 in chronic urticaria group in comparison to control group, with borderline statistical significance. Additionally, we assumed haplotype Gd statistically significant negative chronic urticaria association with tendency to higher frequency of Aw haplotype in this group. Conclusions: The results of our study imply the role of autoimmune components in chronic urticaria pathogenesis and present chronic urticaria as possibly genetically related disorder.
    No preview · Article · May 2013 · Allergologia et Immunopathologia
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    ABSTRACT: The RAC-OST-POL population-based, epidemiological study provided data concerning the influence of education, marital status, occupation, and the place of living (residence) on skeletal status, fracture prevalence, and the course and effectiveness of osteoporotic therapy in 625 women older than 55 years, all of them recruited from the District of Raciborz in Poland. Their mean age was 66.4 ± 7.8 years. All the women completed a specially designed questionnaire. The skeletal status was assessed by femoral neck (FN) and total hip (TH) densitometry, using a Lunar DPX system (USA). In univariate analyses, taking into consideration the age differences, bone mineralization was dependent on marital status (Z score for FN and TH was significantly higher in widows than in divorcees; p < 0.05), place of residence (better results in rural areas; p < 0.05), and occupation (better in standing than sitting jobs; p < 0.05 for FN Z score and p < 0.01 for TH Z score). The multivariate model allowed us to verify that only place of living and type of occupation had a significant influence on densitometry results. In direct comparison, fracture prevalence seemed to be borderline significantly more common in widows (33.5 %) and least common among divorcees (11.8 %) (χ 2 = 6.9, df = 3, p = 0.07), but reanalysis performed after age adjustment excluded a true impact of marital status on fracture occurrence. Other factors did not affect fracture occurrence. Some factors influenced the use of medications for osteoporosis: higher level of education was associated with a more frequent use of vitamin D (χ 2 = 8.49, df = 3, p < 0.05) and of hormone replacement therapy (HRT) (χ 2 = 35.7, df = 3, p < 0.00001). HRT was most commonly used by unmarried women (30 %) and least commonly by divorcees (11.8 %) (χ 2 = 11.7, df = 3, p = 0.01). Vitamin D was more often used among women from the urban area of Raciborz than by those from surrounding rural areas (χ 2 = 9.2, df = 1, p < 0.01). The frequency of use of the three aforementioned medications was associated with the character of occupation. Women with sedentary jobs demonstrated the highest frequency of intake for vitamin D (χ 2 = 9.92, df = 3, p < 0.05) and HRT (χ 2 = 19.48, df = 3, p < 0.001) as well as for other antiresorptive medications (χ 2 = 8.18, df = 3, p < 0.05). We concluded that the results of the epidemiological study demonstrate that both skeletal status and use of antiosteoporotic medications were partially modified by analyzed social factors, whereas fracture prevalence was generally independent from those factors. These data suggest that education, marital status, place of living, and type of occupation may have impacts on implementation of osteoporosis-preventing health programs.
    No preview · Article · May 2013 · Journal of Bone and Mineral Metabolism
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    ABSTRACT: Transforming growth factor β1 (TGF-β1) is a cytokine involved in the process of pathological tissue sclerosis, which is part of the pathophysiological mechanism of end stage renal disease development. The aim of the study was to evaluate the association of the single nucleotide polymorphism rs1800471 of the TGFB1 gene with chronic kidney disease (CKD) occurrence and progression as well as hypertension appearance. It was a case-control study where 109 patients with CKD and 111 very old people were enrolled. The association of the studied polymorphism with mentioned diseases was assessed in the whole study group as well as in the subgroups stratified according to the underlying etiology of CKD: nephropathy in type 1 diabetes (n = 13), chronic glomerulonephritis (n = 50) and chronic interstitial nephritis (n = 46). No association of CKD progression with rs1800471 polymorphism was observed. The C allele was identified as the one associated with higher risk of the disease occurrence in the dominant model of inheritance (p = 0.035). The C allele in women, opposite to male gender, was associated with higher risk of CKD development (p = 0.038). GG genotype was associated with elevated risk of hypertension appearance (p = 0,0021). Due to the lack of accordance with previously performed studies it is still impossible to state an unequivocal conclusion regarding the association between rs1800471 polymorphism of the TGFB1 gene and risk of CKD occurrence and progression as well as hypertension appearance. That is why it is necessary to perform further studies in this field.
    Full-text · Article · Apr 2013 · Archives of Medical Science
  • Z Brzoza · W Grzeszczak · B Rogala · W Trautsolt · D Moczulski
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    ABSTRACT: Background: Autoimmune mechanisms are considered to play a significant role in chronic urticaria pathophysiology. Additionally, clinical experience emphasises the coexistence of chronic urticaria manifestation with thyroid autoimmunity. As the role of CTLA-4 polymorphism in autoimmune thyroid diseases is well proven we speculated on the possible role of this polymorphism in the background of chronic urticaria. Materials and methods: We included 128 chronic spontaneous autoreactive urticaria patients (87 females and 41 males) and 101 healthy volunteers (71 females and 30 males). In all examined subjects CTLA-4 A49G polymorphism was analysed. Disease severity with Urticaria Activity Score as well as age of disease onset was also studied. Results: No statistically significant differences in the allele or genotype distribution between urticaria patients and controls were observed. Furthermore, we found no association between CTLA4 polymorphism and urticaria severity as well as the age of disease onset. Conclusions: Our data suggest that there is no contribution of CTLA-4 A49G polymorphism to chronic spontaneous autoreactive urticaria susceptibility. We recommend further research on other polymorphisms in chronic urticaria patients to explore in detail the potent role of the genetic background in the pathogenesis of this disorder.
    No preview · Article · Apr 2013 · Allergologia et Immunopathologia
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    ABSTRACT: Introduction: Different pathological processes can deteriorate kidney function and cause ireversible degeneration of its structure; however, an optimal way to inhibit or slow down progression of renal damage is unforunately not available. In the light of promissing data concerning homeodomain-interacting protein kinase 2 (HIPK2) upregulation in damaged kidneys animal model, and increased levels of this protein in patients with various kidney diseases, the influence of rs7456421 and rs 2030712 single nucleotide polimorphisms of HIPK2 gene on chronic kidney disease incidence and progression was studied. Material and methods: In 109 family 'trios', consisting of an affected child with CKD (48 females and 61 males, mean age 15.5 ±6.45 years) and both his/her parents, using Transmission Disequilibrium Test allele was used for the transfer of afore-mentioned SNPs from biological parents to their affected offspring. Results: No statistical significance of allele transfer was found, which means that there were no associations between rs7456421 and rs 2030712 SNPs of HIPK2 gene and the incidence of renal dysfunction. Multiple stepwise regression showed a history of chronic glomerulonephritis (OR=17.3), chronic interstitial nephritis without urinary tract defect (OR=4.4), and CT genotype of rs 2030712 SNP (OR=2.6) as determinant of a more rapid progression of renal dysfunction, in contrast to the protective action of body mass index (OR=0.86). Conclusions: On the basis of TDT results, the influence of rs7456421 and rs 2030712 SNPs of HIPK2 gene on prevalence of chronic kidney disease was not identified. Further studies are needed to ascertain the tight relationships of HIPK2 gene polymorphisms with CKD of different etiologies.
    No preview · Article · Mar 2013 · Annals of agricultural and environmental medicine: AAEM

Publication Stats

1k Citations
702.84 Total Impact Points

Institutions

  • 2003-2014
    • Medical University of Silesia in Katowice
      • Department of Nephrology, Endocrinology and Metabolic Diseases
      Catowice, Silesian Voivodeship, Poland
  • 1997-2014
    • Silesian University of Technology
      Gleiwitz, Silesian Voivodeship, Poland
  • 2008
    • Pomorski Uniwersytet Medyczny
      Stettin, West Pomeranian Voivodeship, Poland
    • Charles University in Prague
      Praha, Praha, Czech Republic
  • 2007
    • Maria Sklodowska Curie Memorial Cancer Centre
      Gleiwitz, Silesian Voivodeship, Poland
  • 2004
    • University of Cambridge
      Cambridge, England, United Kingdom
  • 1998-2001
    • Universität Heidelberg
      Heidelburg, Baden-Württemberg, Germany