[Show abstract][Hide abstract] ABSTRACT: Spinal cord injury (SCI) results in a series of severe dysfunction of sensory and motor functions, while the molecular mechanisms that cause these dysfunctions remain elusive. Using proteomics technology, Western blot (WB), and immunohistochemistry (IHC), we found endoplasmic reticulum protein 29 (ERp29) was substantially downregulated in the motor cortex 3 days postoperation (dpo) after spinal cord transection (SCT, T10) followed by a gradual recovery 28 dpo. IHC showed that ERp29 is expressed in cortical neurons. In order to investigate the role of ERp29 in axotomized cortical neurons, we developed an in vitro axotomy injury model. ERp29 overexpression in cortical neurons after axotomy protected them from apoptosis; prevented the reduction of the number of neurons, and prevented reduction of neurite length. Moreover, we found that ERp29 overexpression increased neuronal regeneration assessed by neurite number and length. Furthermore, overexpression of ERp29 in cortical neurons after axotomy increased expression of Erk-1 and PI3K while decreasing the expression of caspase-3 expression. The present data therefore provides evidence to address the role of ERp29 in axotomized cortical neurons and identifies new therapeutic targets for the treatment of SCI.
[Show abstract][Hide abstract] ABSTRACT: Spinal cord injury (SCI) often results in motor disability concomitant with limit neuroplasticity; the underlying mechanism, however, is still unclear. This study established spinal cord transection rats model (T10), then performed cDNA microarray analysis and found that vimentin located in astrocytes was increased significantly in scar tissues after transection. To understand the role of vimentin and it's mechanism of regulation, RNA interference and luciferase assay were used. Vimentin knockdown in the scar tissues showed a significant improvement on locomotor function in hindlimbs, while vimentin overexpression exhibited an opposite effect. In vitro, vimentin downregulation or overexpression can effectively inhibit or increase astrogliosis, respectively. Moreover, by using biological informatics technology, we predicted that vimentin may be as the target of micRNA138 (miR-138), and confirmed that miR-138 could regulate vimentin by luciferase activity assay. The present results not only validated the exact role of vimentin in transected spinal cord, but also exhibited a novel regulation mechanism, in which miR-138 may regulate vimentin to promote neuroplasticity. It, therefore, provides a novel target for gene drug discovery based on miRNA-138 or vimentin for the treatment of SCI in the future clinic trial.
No preview · Article · May 2014 · Molecular Neurobiology
[Show abstract][Hide abstract] ABSTRACT: To investigate the expression of brain-derived neurotrophic factor (BDNF) in lung injury induced by brain ischemia in rats.
46 adult SD rats were assigned randomly to sham operation group and brain ischemia lung injury group (BILI, n = 23 in each group). Rats were subjected brain ischemia and allowed to survived 3 d. After performed neurological functional severe deficit evaluation, lung edema was observed (n=5). The BDNF expression for its mRNA and protein in lung tissues was determined by using ELISA (n=5) and RT-PCR technique (n=8). The localization of BDNF was also determined by immunohistochemistry (n=5).
After brain ischemia for 3 days, the severe neurological functional deficit and edema in lung were seen. BDNF was located in cytoplasma of smooth muscle and epithial cells in the lung. The level of BDNF mRNA (indicated by RT-PCR) and the protein level (indicated by ELISA) were all upregulated at 3 days after brain ischemia (P<0.05).
Lung edema occurred after brain ischemia in rats is concomitant with BDNF expression, which consists of the mechanism involved lung injury induced by brain ischemia.
No preview · Article · Nov 2012 · Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
[Show abstract][Hide abstract] ABSTRACT: It is well known that neuroplasticity occurs in the central nervous system in response to injury. Electro-acupuncture (EA) may also promote neuroplasticity. But little is known about the underlying molecular mechanisms for the beneficial effects of EA. This study investigated the effects of EA on neurotrophin-4 (NT-4) expression in L6 spinal dorsal root ganglion (DRG) and associated segments of the spinal dorsal horn in cats subjected to unilateral removal of L1–L5 and L7–S2 DRG. NT-4 protein was normally present in the cytoplasm of the L6 DRG neurons and L3 and L6 spinal dorsal horn neurons and glia. Adjacent ganglionectomy leads to a significant decrease in NT-4 expression in the L6 DRG, but no change in the spinal dorsal horn. Following EA treatment a significant increase occurred in the L6 DRG at 14 days post-operation (dpo) as well as the L6 cord segment at 7 and 14 dpo. These findings pointed to a possible association between NT-4 expression and EA promoted spinal cord plasticity in adult cats subjected to partial ganglionectomy.
Full-text · Article · Oct 2012 · Neuroscience Letters
[Show abstract][Hide abstract] ABSTRACT: Spatiotemporal changes of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) in the spinal cords of chick embryonic stage day 7 (E7) and day 14 (E14) were examined by using immunohistochemistry and Western blot. Intensive NGF immunoreaction (IR) was detected in the white matter of the spinal cords, while BDNF-IR in perikaryon and neurite, and NT-3-IR in the nucleus and cytoplasm were seen in the neurons of the ventral horn in the gray matter. Comparatively, the expressions for three growth factors have expanded largely into the dorsal horn at E14, and the level of proteins for these growth factors increased significantly in the spinal cords from E7 to E14. Morphological observation showed that the lumbar spinal cords of E7 appeared rectangular, whereas it gave a butterfly shape in the gray matter consisting of the typical ventral horn, dorsal horn and intermediate zone at E14. The present findings indicated that the spatiotemporal changes of NGF, BDNF and NT-3 could be associated to the morphological changes of developing spinal cords, suggesting the possible roles of three growth factors in the development of spinal cords.
No preview · Article · Oct 2009 · Neurochemical Research
[Show abstract][Hide abstract] ABSTRACT: The effects of electro-acupuncture (EA) on insulin-like growth factor-I (IGF-I) expression in the spared dorsal root ganglia (DRG) and associated spinal dorsal horns were explored in cats subjected to unilateral removal of L(1)-L(5) and L(7)-S(2) DRG, sparing the L(6) DRG. Immunohistochemistry revealed the presence of IGF-I immunoreactive products in the L(6) DRG neurons and some neurons and glial cells in the spinal cord. Western blot demonstrated that the level of IGF-I was significantly up-regulated both in the spared DRG and the dorsal horns of L(3) and L(6) cord segments at both 7 and 14 days post operation following EA. The present findings demonstrated the association between neuroplasticity and IGF-I expression, suggesting the possible role of IGF-I in EA promoted spinal cord plasticity.
No preview · Article · Jun 2009 · Neurochemical Research