Karuppan Muthukumar

Anna University, Chennai, Chennai, Tamil Nadu, India

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Publications (56)140.68 Total impact

  • V. P. Manjamadha · Karuppan Muthukumar
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    ABSTRACT: This study presents the facile, green and eco-friendly synthesis of silver nanoparticles (AgNPs) using weed plant Lantana camara L. leaf extract. The incorporation of ultrasound into this reduced the time and increased the reaction rate. The results showed that the AgNPs were spherical in shape with the average size of 33.8 nm. The EDAX pattern indicated the presence of abundant silver and XRD indicated that the (111) crystallographic plane more predominant than other planes. The possible functional groups responsible for the reduction and stabilization of AgNPs were identified using Fourier transform infrared spectroscope. The XPS results concluded that the nanoparticles were presented in its reduced metallic state. The antioxidant activity of AgNPs was assayed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) test. The increase in the concentration of AgNPs increased the DPPH scavenging activity. The AgNPs revealed superior antibacterial activity against Gram positive and Gram negative organisms.
    No preview · Article · Jan 2016 · Bioprocess and Biosystems Engineering
  • Fareeda Chemat · Hor Jun You · Karuppan Muthukumar · Thanapalan Murugesan
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    ABSTRACT: In the present work, DESs composed of choline chloride (ChCl), glycerol and l-arginine at various molar ratios were synthesized and the effect of temperatures and molar ratios of glycerol and l-arginine on their physical properties were studied. Densities and viscosities were measured at the temperature range from 298.15 K to 343.15 K, whereas refractive index was measured at 298.15 K to 333.15 K. The density values were used to estimate thermal expansion coefficients while the viscosity values were used to estimate activation energies. The thermal stability of the mixtures was found to be more dependent on the concentration of glycerol and l-arginine.
    No preview · Article · Dec 2015 · Journal of Molecular Liquids
  • Balasubramani Ramprakash · Karuppan Muthukumar
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    ABSTRACT: This study compares the performance of different pretreatment and hydrolysis methods for the efficient production of fermentable sugars from rice mill wastewater. The pretreatment and hydrolysis operating conditions were optimized. The maximum reducing sugar concentration of 16.1 g/L was obtained with combined acid and enzymatic hydrolysis. The heat, alkali, ultrasound pretreatment, acid hydrolysis and enzymatic hydrolysis showed 2.9, 2.7, 2.1, 14.2 and 15.8 g/L reducing sugar concentration, respectively. The hydrogen production was carried out using Enterobacter aerogenes RM 08 with hydrolyzate obtained from combined acid and enzymatic hydrolysis and the results showed 1.97 mol H2/mol of sugar. The cumulative hydrogen production and the growth data of E. aerogenes RM 08 were fitted with modified Gompertz and Logistic equations.
    No preview · Article · Aug 2015 · International Journal of Hydrogen Energy
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    Govindarajan Ramadoss · Karuppan Muthukumar
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    ABSTRACT: This study presents the ultrasound assisted pretreatment of sugarcane bagasse (SCB) using metal salt with hydrogen peroxide for bioethanol production. Among the different metal salts used, maximum holo-cellulose recovery and delignification were achieved with ultrasound assisted titanium dioxide (TiO2) pretreatment (UATP) system. At optimum conditions (1% H2O2 , 4 g SCB dosage, 60 min sonication time, 2:100 M ratio of metal salt and H2O2 , 75°C, 50% ultrasound amplitude and 70% ultrasound duty cycle), 94.98 ± 1.11% holocellulose recovery and 78.72 ± 0.86% delignification were observed. The pretreated SCB was subjected to dilute acid hydrolysis using 0.25% H2SO4 and maximum xylose, glucose and arabinose concentration obtained were 10.94 ± 0.35 g/L, 14.86 ± 0.12 g/L and 2.52 ± 0.27 g/L, respectively. The inhibitors production was found to be very less (0.93 ± 0.11 g/L furfural and 0.76 ± 0.62 g/L acetic acid) and the maximum theoretical yield of glucose and hemicellulose conversion attained were 85.8% and 77%, respectively. The fermentation was carried out using Saccharomyces cerevisiae and at the end of 72 h, 0.468 g bioethanol/g holocellulose was achieved. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis of pretreated SCB was made and its morphology was studied using scanning electron microscopy (SEM). The compounds formed during the pretreatment were identified using gas chromatography–mass spectrometry (GC–MS) analysis.
    Full-text · Article · Aug 2015 · Ultrasonics Sonochemistry
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    Govindarajan Ramadoss · Karuppan Muthukumar

    Full-text · Dataset · Aug 2015
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    Palanisamy Gowthami · Karuppan Muthukumar · Manickam Velan
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    ABSTRACT: The overproduction of enzymes was performed by manipulating the medium components. In our study, solvent-tolerant thermophilic lipase-producing Bacillus coagulans was isolated from soil samples and a stepwise optimization strategy was employed to increase the lipase production using coconut oil cake basal medium. In the first step, the influence of pH, temperature, carbon source, nitrogen source and inducers on lipase activity was investigated by the One-Factor-At-A-Time (OFAT) method. In the second step, the three significant factors resulted from OFAT were optimized by the statistical approach (CCD).The optimum values of olive oil (0.5%), Tween 80 (0.6%) and FeSO4 (0.05%) was found to be responsible for a 3.2-fold increase in the lipase production identified by Central Composite Design.
    Preview · Article · Jul 2015
  • Balasubramani Ramprakash · Karuppan Muthukumar
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    ABSTRACT: This study presents the biohydrogen production from rice mill wastewater using mutated Enterobacter aerogenes obtained from random mutagenesis of wild E. aerogenes using UV and ethidium bromide (EtBr). The mutants obtained were screened based on sugar utilization efficiency and the best mutant was selected for further studies. Maximum hydrogen production of 1.92 mol H2/mol sugar was observed with EtBr mutant, whereas 1.72 mol H2/mol sugar was observed with UV mutant. Mutant strains showed 25–35% enhanced hydrogen production than wild strain and the optimum initial pH was found to be 7.0. An efficient mutant strain, E. aerogenes RM08, showed improved growth and enzyme production compared to wild strain. The production hydrogenase was confirmed by SDS-PAGE and enzyme sequencing using MALDI-TOF MS. The hydrogen production and growth data obtained were fitted with Modified Gompertz and Logistic equation. The chemical oxygen demand (COD) reduction of 81% was obtained at the end of 60 h of fermentation.
    No preview · Article · Jul 2015
  • Zerihun Demrew Yigezu · Karuppan Muthukumar
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    ABSTRACT: Structural features of metal oxides are the primary motives for utilizing them in a number of reactions and hence, investigation of catalyst structure-performance relationship is essential. This study deals with the morphological characterization of the catalysts used (Co3O4, V2O5 and ZnO) for the catalytic pyrolysis of sunflower oil for biofuel production. Also, the % organic liquid product (OLP) and fractional distillates obtained for different catalysts were analyzed. The best catalytic activity was observed with V2O5 and it was found to give higher OLP yield and gasoline like fraction. Scanning electron microscope (SEM) images of the catalysts were analyzed to determine the structural modifications of the catalysts during the reaction. The Co3O4 particles attained irregular shapes after use whereas marginal structural changes were observed for ZnO. The most fragile portion of the V2O5 particles was found to be partially eliminated upon use. FTIR analysis revealed the presence of aliphatic esters and derivatives of esters in the synthesized OLP and its gasoline fraction. The properties such as density, specific gravity, kinematic viscosity, flash point and higher heat value of the product were within the limits of ASTM D6751 (B100) standards.
    No preview · Article · May 2015 · Journal of Analytical and Applied Pyrolysis
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    Govindarajan Ramadoss · Karuppan Muthukumar
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    ABSTRACT: This study presents the influence of different dual salts on the pretreatment of sugarcane bagasse (SCB) with H2O2 for the production of bioethanol. The results showed better cellulose recovery and delignification for MnSO4·H2O and ZnO system. At optimized conditions (1% H2O2, 1 g SCB, 30 min, 100 °C and 0.5:100 g/mL), the maximum cellulose recovery of 93.42 ± 1.74%, and delignification of 74.18 ± 1.51% were obtained. The SCB was subjected to dilute acid hydrolysis, and under optimized conditions, the maximum glucose, xylose and arabinose concentrations obtained were 15.26 ± 0.37 g/L, 11.62 ± 0.21 g/L and 3.58 ± 0.17 g/L, respectively. The results of SEM, FTIR and XRD analysis suggested that the proposed method effectively removed lignin and disrupted the crystal structure of cellulose for a better hydrolytic process. The fermentation of hydrolyzate with Saccharomyces cerevisiae showed maximum bioethanol production of 13.1 g/L at the end of 72 h and bioethanol yield of 0.184 g/g SCB.
    Full-text · Article · Jan 2015 · The Chemical Engineering Journal
  • S R Vijayalakshmidevi · Karuppan Muthukumar
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    ABSTRACT: The present study demonstrates the de-colorization and degradation of textile effluent by coculture consisting of three bacterial species isolated from textile effluent contaminated environment with an aim to reduce the treatment time. The isolates were identified as Ochrobactrum sp., Pseudomonas aeruginosa and Providencia vermicola by 16S rRNA analysis. Their secondary structure was predicted and GC content of the sequence was found to be 54.39, 52.10, and 52.53%. The co-culture showed a prominent increase in the degradation activity due to the action of oxidoreductase enzymatic mechanism of laccase, NADH-DCIP reductase and azoreductase activity. The biodegradability index of 0.75 was achieved with 95% chemical oxygen demand (COD) reduction in 16h and 78 and 85% reduction in total organic carbon (TOC) and total solids was observed. Bioaccumulation of metals was identified by X-ray diffraction (XRD) analysis. The effective decolorization was confirmed from the results of UV-vis spectroscopy, high performance liquid chromatography and Fourier transformed infrared spectrometer analyzes. The possible degradation pathway was obtained from the analysis of liquid chromatography-mass spectroscopy analysis and the metabolites such as 2-amino naphthalene and N-phenyl-1.3,5 triazine were observed. The toxic nature of the effluent was analyzed using phyto-toxicity, cell-death assay and geno-toxicity tests. Copyright © 2014 Elsevier Inc. All rights reserved.
    No preview · Article · Jan 2015 · Ecotoxicology and Environmental Safety
  • Zerihun Demrew Yigezu · Karuppan Muthukumar
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    ABSTRACT: This study presents the kinetic aspects of organic liquid products (OLP) synthesis from sunflower oil by catalytic cracking. A three-lump reaction kinetic model and Arrhenius equation were used to estimate the reaction constants and activation energy values. The effect temperature and catalyst concentration on % conversion and product distribution were analyzed. The maximum OLP formation (92.1%) was obtained at 628 K with 1.5 wt.% catalyst in 40 min. The data predicted by 3-lump kinetic model were well consistent with the experimental values. The smaller value of reaction constant obtained for the conversion of OLP into gas and coke indicates the presence of little secondary reactions. The lower activation energy required to convert the oil into OLP implied the selectivity of the catalyst towards OLP formation. The measured physical properties of the OLP were within/close to the required limits of ASTM D6751 (B100) specifications. FTIR and GC–MS analysis identified aliphatic ester and their derivatives as major constituents of the OLP. Variations in the fractional distillates of OLP indicate the influence of reaction conditions not only on % conversion but also on product distribution.
    No preview · Article · Jan 2015 · Journal of Analytical and Applied Pyrolysis
  • Senthilnathan Nachiappan · Karuppan Muthukumar
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    ABSTRACT: This study presents the treatment of a high strength pharmaceutical wastewater by sono-Fenton-sorption (SFS). Better results were observed with SFS compared to individual processes and the maximum chemical oxygen demand reduction observed after 180 min of treatment with SFS, sono-Fenton and sono-sorption was 71.9, 48.4, and 43.75%, respectively. The effect of parameters such as initial concentration, dosage of Fenton's reagent, sorbent dosage, and pattern of addition of iron on the performance of SFS was analyzed. The SS and SFS were found to fit well with Langmuir-Hinshelwood kinetics. Fourier transformed infrared studies of effluent and sorbent before and after treatment confirmed the oxidative degradation of pharmaceutical effluent. Toxicity of the effluent was considerably reduced after the treatment and the electrical energy consumption was significantly reduced, when sonolysis was coupled with sorption and Fenton's process (SFS).
    No preview · Article · Nov 2014 · CLEAN - Soil Air Water
  • Mujyambere Jean Marie Vianney · Karuppan Muthukumar
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    ABSTRACT: This study presents the removal of azo dyes (Congo red, Methyl red and Eriochrome black T) from aqueous solutions by ultrasound assisted electrocoagulation (UE). In this method, dye solutions were simultaneously sonicated and electrolyzed. The effect of various operating parameters such as intensity of irradiation, pH, current intensity, initial concentration of the dye and temperature on the performance of the process was investigated. The reduction in chemical oxygen demand, biological oxygen demand, hardness and alkalinity were assessed. The kinetic analysis of the data showed a pseudo-first order degradation for all three dyes. The results showed that UE is an effective method for the destruction of dyes.
    No preview · Article · Oct 2014 · CLEAN - Soil Air Water
  • Balasubramani Ramprakash · Karuppan Muthukumar
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    ABSTRACT: This study presents the production of biohydrogen from rice mill wastewater. The acid hydrolysis and enzymatic hydrolysis operating conditions were optimized, for better reducing sugar production. The effect of pH and fermentation time on biohydrogen production from acid and enzymatic hydrolyzed rice mill wastewater was investigated, using Enterobacter aerogenes and Citrobacter ferundii. The enzymatic hydrolysis produced the maximum reducing sugar (15.8 g/L) compared to acid hydrolysis (14.2 g/L). The growth data obtained for E. aerogenes and C. ferundii, fitted well with the Logistic equation. The hydrogen yields of 1.74 mol H2/mol reducing sugar, and 1.40 mol H2/mol reducing sugar, were obtained from the hydrolyzate obtained from enzymatic and acid hydrolysis, respectively. The maximum hydrogen yield was obtained from E. aerogenes compared to C. ferundii, and the optimum pH for better hydrogen production was found to be in the range from 6.5 to 7.0. The chemical oxygen demand (COD) reduction obtained was around 71.8% after 60 h of fermentation.
    No preview · Article · Sep 2014 · International Journal of Hydrogen Energy
  • S.R. Vijayalakshmidevi · Karuppan Muthukumar
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    ABSTRACT: This study presents the phytoremediation of textile effluent pretreated with ultrasound irradiation and microbial treatment. The isolates such as Ochrobactrum sp., Pseudomonas aeruginosa and Providencia vermicola were used for the bacterial degradation and phytoremediation was done using Bouteloua dactyloides. The effect of sonication time on the performance of phytoremediation was evaluated. Ochrobactrum sp. showed better performance compared to other species and better results were observed with the effluent sonicated for a longer period. The pretreatment of textile effluent with Ochrobactrum sp. and sonication improved the phytoremediation and showed about 95% COD reduction at the end of 24 h. The influence of oxidoreductive enzymes such as laccase, NADH–DCIP reductase and azoreductase during bacterial degradation was studied and the activity of catalase and peroxidase was identified during phytoremediation. The UV–visible and high performance liquid chromatography analysis confirmed the degradation of dye molecules. The possible metabolic pathway was identified through liquid chromatography-mass spectroscopy analysis. The X-ray diffraction (XRD) analysis showed the reduction in the heavy metals content of the treated effluent and the phytotoxicity analysis confirmed the less toxic nature of the treated effluent.
    No preview · Article · Sep 2014 · Journal of Environmental Chemical Engineering
  • Zerihun Demrew Yigezu · Karuppan Muthukumar
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    ABSTRACT: This study presents the utilization of metal oxides for the biofuel production from vegetable oil. The physical and chemical properties of the diesel-like products obtained, and the influence of reaction variables on the product distribution were investigated. Six different metal oxides (Co3O4, KOH, MoO3, NiO, V2O5, and ZnO) were employed as catalysts and the results indicated that the metal oxides are suitable for catalyzing the conversion of oil into organic liquid products (OLPs). The maximum conversion (87.6%) was obtained with V2O5 at 320 °C in 40 min whereas a minimum conversion (55.1%) was obtained with MoO3 at 390 °C in 30 min. The physical characteristics of the product obtained (density, specific gravity, higher heat value, flash point and kinematic viscosity), were in line with ASTM D6751 (B100) standards. The hydrocarbons majorly present in the product were found to be methyl and ethyl esters. Furthermore, OLPs obtained were distilled and separated into four components. The amount of light hydrocarbons, gasoline, kerosene and heavy oil like components obtained were 18.73%, 33.62%, 24.91% and 90.93%, respectively.
    No preview · Article · Aug 2014 · Energy Conversion and Management
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    Arjunan Babuponnusami · Karuppan Muthukumar
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    ABSTRACT: The increase in the disposal of refractory organics demands for newer technologies for the complete mineralization of these wastewaters. Advanced oxidation processes (AOPs) constitute a promising technology for the treatment of such wastewaters and this study presents a general review on such processes developed to decolorize and/or degrade organic pollutants. Fundamentals and main applications of typical methods such as Fenton, electro-Fenton, photo-Fenton, sono-Fenton, sono-photo-Fenton, sono-electro-Fenton and photo-electro-Fenton are discussed. This review also highlights the application of nano-zero valent iron in treating refractory compounds.
    Preview · Article · Mar 2014 · Journal of Environmental Chemical Engineering
  • S R Vijayalakshmidevi · Karuppan Muthukumar
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    ABSTRACT: This study presents the biodegradation of malachite green (MG), a triphenylmethane dye, using a novel microorganism isolated from textile effluent contaminated environment. The organism responsible for degradation was identified as Ochrobactrum sp JN214485 by 16S rRNA analysis. The effect of operating parameters such as temperature, pH, immobilized bead loading, and initial dye concentration on % degradation was studied, and their optimal values were found to be 30 °C, 6, 20 g/L and 100 mg/L, respectively. The analysis showed that the extracellular enzymes were responsible for the degradation. The biodegradation of MG was confirmed by UV-visible spectroscopic and FTIR analysis. The phytotoxicity test concluded that the degradation products were less toxic compared to MG. The kinetics of biodegradation was studied and the activation energy was found to be 10.65 kcal/mol.
    No preview · Article · Aug 2013 · World Journal of Microbiology and Biotechnology (Formerly MIRCEN Journal of Applied Microbiology and Biotechnology)
  • Ramachandran Sivaramakrishnan · Karuppan Muthukumar
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    ABSTRACT: This work emphasizes the potential of the isolated Bacillus sp. lipase for the production of fatty acid methyl ester by the direct transesterification of Oedogonium sp. of macroalgae. Dimethyl carbonate was used as the extraction solvent and also as the reactant. The effect of solvent/algae ratio, water addition, catalyst, temperature, stirring and time on the direct transesterification was studied. The highest fatty acid methyl ester yield obtained under optimum conditions (5 g Oedogonium sp. powder, 7.5 ml of solvent (dimethyl carbonate)/g of algae, 8% catalyst (%wt/wt of oil), distilled water 1% (wt/wt of algae), 36 h, 55°C and 180 rpm) was 82%. Final product was subjected to thermogravimetric analysis and (1)H NMR analysis. The results showed that the isolated enzyme has good potential in catalyzing the direct transesterification of algae, and the dimethyl carbonate did not affect the activity of the isolated lipase.
    No preview · Article · Jul 2013 · Journal of Bioscience and Bioengineering
  • Dharmaraj Gunasundari · Karuppan Muthukumar
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    ABSTRACT: This study presents simultaneous hexavalent chromium (Cr(VI)) reduction and phenol degradation using Stenotrophomonas sp., isolated from tannery effluent contaminated soil. Phenol was used as the sole carbon and energy source for Cr(VI) reduction. The optimization of different operating parameters was done using Placket-Burman design (PBD) and Box-Behnken design (BBD). The significant operating variables identified by PBD were initial Cr(VI) and phenol concentration, pH, temperature, and reaction time. These variables were optimized by a three-level BBD and the optimum initial Cr(VI) concentration, initial phenol concentration, pH, temperature, and reaction time obtained were 16.59 mg/l, 200.05 mg/l, 7.38, 31.96 °C and 4.07 days, respectively. Under the optimum conditions, 81.27 % Cr(VI) reduction and 100 % phenol degradation were observed experimentally. The results concluded that the Stenotrophomonas sp. could be used to decontaminate the effluents containing Cr(VI) and phenol effectively.
    No preview · Article · Apr 2013 · Environmental Science and Pollution Research