A E Bugrova

Russian Academy of Sciences, Moskva, Moscow, Russia

Are you A E Bugrova?

Claim your profile

Publications (17)24.74 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Nitric oxide is a universal molecule which regulates numerous functions in the body. In the retina, nitric oxide plays a regulatory role modulating synaptic transmission between photoreceptors and bipolar cells; it also has a toxic role and is a necessary inducer of the development of apoptosis in the outer nuclear layer and the ganglion cell layer. We report here studies of the endogenous nitric oxide concentration in vivo in the cellar layers of the retina. Nitric oxide concentrations were determined by two independent methods – using a spin trap and electron paramagnetic resonance (EPR) and an NO-sensitive microelectrode, to obtain in situ measurements in the live isolated frog retina. Significant nitric oxide concentrations were seen only in the ganglion cell layer (~0.25 μM) and the inner segments of photoreceptors (~0.6 μM). NO synthase was present in the same locations; the activity and kinetic parameters of this enzyme were also determined.
    No preview · Article · Jan 2016 · Neuroscience and Behavioral Physiology
  • [Show abstract] [Hide abstract]
    ABSTRACT: Nitric oxide is a universal molecule that regulates many different functions in an organism. In the eye retina nitric oxide plays both a regulatory role by modulation of the synaptic transmission between photoreceptors and bipolar cells and a toxic role in apoptosis induction in the outer nuclear layer and in the layer of ganglion cells. In this paper there has been made the first attempt to estimate the endogenous NO concentration in retina layers in vivo. The concentration of the nitric oxide was determined by two indepen- dent techniques: ESR spectrometry using spin trap for in vivo determination and NO-sensitive microelectrode for in situ determination in the survival isolated frog retina. The distinct NO con- centration was detected only in the ganglion cells layer (~0.25 μM) and in the inner segments layer of the photoreceptors (~0.6 μM). The activity and the kinetic characteristic of the NO-synthase localized in the same layers were also determined. Key words: retina cells layers, nitric oxide, ESR, NO-sensitive microelectrodes.
    No preview · Article · Feb 2015 · Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk
  • [Show abstract] [Hide abstract]
    ABSTRACT: The variability of control samples is the main problem in clinical proteomics and reliable sources for estimation of variability of normal content of different proteins are not numerous. Most of the investigations of normal human tear proteome include pool of samples as reference materials. But it is impossible to use such experimental approach to establish the range of variability of different tear proteome components. This study is the first attempt to estimate the variability of proteins content in healthy donors tear using high-performance liquid chromatography and tandem mass spectrometry. The protein profiles from 11 individual healthy donors were analyzed. Essential variability of the minor proteins was revealed while the presence of 6 major proteins in all 11 samples was invariable. We found the Lacritin glycosylation in all samples of tear fluid received from healthy donors. It seems that this modification is typical for healthy donors tear. The analysis of the pool samples was also carried out to estimate the availability for this approach for the search of ophthalmologic diseases biomarkers.
    No preview · Article · Apr 2013 · Rossiĭskii fiziologicheskiĭ zhurnal imeni I.M. Sechenova / Rossiĭskaia akademiia nauk
  • [Show abstract] [Hide abstract]
    ABSTRACT: It was shown that retinal ischemia entailed apoptosis in the inner layers of the retina. Administration of an NO-synthase inhibitor suppressed the development of ischemic apoptosis. To ascertain whether nitric oxide could induce the retinal apoptosis by itself, a nontoxic NO donor—dinitrosyl iron complex (DNIC) with glutathione—was injected into the vitreous body. DNIC at low concentrations induced apoptosis in the same retinal layers as in ischemia. However, with increasing DNIC doses, the number of apoptotic nuclei decreased markedly. Simultaneous administration of excess glutathione prevented apoptosis at any DNIC dose. The obtained data demonstrate the neurotoxic properties of the excess of nitric oxide in the retina.
    No preview · Article · Mar 2012 · Biophysics
  • [Show abstract] [Hide abstract]
    ABSTRACT: Using model elaborated it was shown that the retinal ischemia initiated the development of the apoptosis in the inner layers of the retina. Administration of NOS inhibitor prevented the development of the apoptosis in the retina. To ascertain if nitric oxide could induce the retinal apoptosis by itself the nontoxic donor of nitric oxide (dinitrosyl iron complex) was injected intravitreally. Administration of DNIC in low concentrations induced the development of the apoptosis in the same retinal layers as in ischemia. The injection of dinitrosyl iron complex at the higher concentration resulted in the decrease of the apoptosis level. Administration of dinitrosyl iron complex with excess of glutathione didn't lead to the development of the retinal apoptosis. The obtained data demonstrates the neurotoxic properties of the excess of nitric oxide in the retina.
    No preview · Article · Mar 2012 · Biofizika
  • [Show abstract] [Hide abstract]
    ABSTRACT: Gravitational unloading causes atrophy of muscle fibers and can lead to destruction of cytoskeletal and contractile proteins. Along with the atrophic changes, unloaded muscle frequently demonstrates significant shifts in the ratio of muscle fibers expressing fast and slow myosin heavy chain isoforms. Stretching of the m. soleus during hindlimb suspension prevents its atrophy. We supposed that neuronal NO-synthase (NOS) (which is attached to membrane dystrophin-sarcoglycan complex) can contribute to maintenance of protein metabolism in the muscle and prevent its atrophy when m. soleus is stretched. To test this hypothesis, we used Wistar rats (56 animals) in experiments with hindlimb suspension during 14 days. The group of hindlimb suspended rats with stretched m. soleus was injected with L-NAME to block NOS activity. We found that m. soleus mass and its protein content in hindlimb-suspended rats with stretched m. soleus were preserved due to prevention of protein degradation. NOS is involved in maintenance of expression of some muscle proteins. Proliferation of satellite cells in stretched m. soleus may be due to nNOS activity, but maintenance of muscle mass upon stretching is regulated not by NOS alone.
    No preview · Article · Feb 2012 · Biochemistry (Moscow)
  • [Show abstract] [Hide abstract]
    ABSTRACT: Gravitational unloading results in pronounced atrophy of m.soleus. Probably, the output of NO is controlled by the muscle activity. We hypothesized that NO may be involved in the protein metabolism and increase of its concentration in muscle can prevent atrophic changes induced by gravitational unloading. In order to test the hypothesis we applied NO donor l-arginine during gravitational unloading. 2.5-month-old male Wistar rats weighing 220–230g were divided into sedentary control group (CTR, n=7), 14-day hindlimb suspension (HS, n=7), 14 days of hindlimb suspension+l-arginine (HSL, n=7) (with a daily supplementation of 500 mg/kg wt l-arginine) and 14 days of hindlimb suspension+l-NAME (HSN, n=7) (90 mg/kg wt during 14 days). Cross sectional area (CSA) of slow twitch (ST) and fast twitch (FT) soleus muscle fibers decreased by 45% and 28% in the HS group (p<0.05) and 40% and 25% in the HSN group, as compared to the CTR group (p<0.05), respectively. CSA of ST and FT muscle fibers were 25% and 16% larger in the HSL group in comparison with the HS group (p<0.05), respectively. The atrophy of FT muscle fibers in the HSL group was completely prevented since FT fiber CSA had no significant differences from the CTR group. In HS group, the percentage of fibers revealing either gaps/disruption of the dystrophin layer of the myofiber surface membrane increased by 27% and 17%, respectively, as compared to the controls (CTR group, p<0.05). The destructions in dystrophin layer integrity and reductions of desmin content were significantly prevented in HSL group. NO concentration decreased by 60% in the HS group (as well as HSN group) and at the same time no changes were detectable in the HSL group. This fact indicates the compensation of NO content in the unloaded muscle under l-arginine administration. The levels of atrogin-1 mRNA were considerably altered in suspended animals (HS group: plus 27%, HSL group: minus 13%) as compared to the control level. Conclusion: l-arginine administration allows maintaining NO concentration in m.soleus at the level of cage control group, prevents from dystrophin layer destruction, decreases the atrogin mRNA concentration in the muscle and atrophy level under gravitational unloading.
    No preview · Article · May 2011 · Acta Astronautica
  • [Show abstract] [Hide abstract]
    ABSTRACT: Cytoskeletal and contractile proteins degenerate during functional unloading of muscle. The ratio of myosin heavy chain (MHC) expression changes simultaneously. We have supposed that NO can be a signal molecule related to the regulation of protein metabolism upon muscle unloading. To test this hypothesis, Wistar rats underwent functional unloading for 14 days without and with peroral administration of L-arginine (500 mg/kg) as NO precursor. Significant decreases in m. soleus mass, NO, nNOS, dystrophin, Hsp90, p-S6K, and type I MHC mRNA contents were found in the group of animals with unloading without preparation compared to those in control and in the group with unloading and administration of L-arginine; at the same time, increased contents of atrogin-1/MAFbx and MuRF-1 (p < 0.05) were found. No difference in the IGF-1 mRNA content between all three groups was found. Atrophy was significantly less pronounced in the group with unloading and L-arginine administration compared to that without the amino acid, and no destruction of cytoskeletal proteins was observed. We conclude that administration of L-arginine upon functional unloading decreases the extent of m. soleus atrophy, prevents the decrease in it of type I MHC mRNA, and blocks destructive changes in some cytoskeletal proteins. Such effect can be due to the absence of increase in this group of the content of some ubiquitin ligases and decreased intensity of the p70S6 kinase synthesis marker.
    No preview · Article · May 2011 · Biochemistry (Moscow)
  • [Show abstract] [Hide abstract]
    ABSTRACT: The possibility and the mechanism of the reduction of nitrites in retinal vessels under the acute hypoxia in vivo have been investigated. An experimental model of the rat retina ischemia was elaborated using the laser coagulation of retinal vessels. It was demonstrated that the vessel thrombosis does not occur if the nitrite concentration in the vessels is increased. It was proposed that, under acute hypoxia, nitrites are reduced to NO, which results in a drastic vasodilatation. Considering that the effect takes less than a minute, this reduction cannot be due to hypoxic acidosis but is more likely associated with NO reduction by heme proteins. It was found that the increased concentration of nitrites protects the retina from the development of ischemia progress and that the preliminary administration of nitrites prevents apoptosis in the retina and a decrease in its photoelectric activity.
    No preview · Article · Aug 2010 · Biofizika

  • No preview · Article · Jun 2010 · Doklady Biological Sciences
  • [Show abstract] [Hide abstract]
    ABSTRACT: An in vivo study was performed to see whether deterioration of the muscle cytoskeleton caused by eccentric exercise could be counteracted by raising the tissue content of nitric oxide. In Wistar rats that ran downhill on a treadmill inclined at 16° for 40 min at 20 m/min, the desmin content in m. soleus measured 24 h later declined by 15%, and the percentage of ruptures in the dystrophin layer was three times higher than in the control. Destruction of cytoskeletal proteins was also pronounced in rats pretreated with a blocker of NO synthase before exercise. By contrast, animals that received a nitric oxide donor (L-arginine) prior to running had control levels of desmin and dystrophin. It was concluded that nitric oxide can protect muscle cytoskeletal proteins in a single eccentric exercise.
    No preview · Article · Jun 2009 · Biophysics
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This review focuses on some intermediate results on the path from the gene and enzyme structure to physiological responses and personalised medicine. Bioinformatics of genetic and protein-structural polymorphisms, theoretical methods of predicting the influence of single amino acid substitutions on the structure and catalytic activity of enzymes are considered. For a large group of enzymes, interrelations between genetic modifications, structural changes of the proteins and the detected physiological and clinical manifestations are discussed. In this respect, highly productive techniques to determine the catalytic activity of an enzyme as well as non-invasive proteomic approaches are of particular interest. A non-invasive proteomic analysis using mass-spectrometric protein identification of human exhaled breath condensate and tear fluids has been chosen.
    Full-text · Article · Jun 2009 · Biotechnology advances
  • [Show abstract] [Hide abstract]
    ABSTRACT: We studied the development of retinal ischemia in rat eye after laser coagulation of blood vessels. Typical signs of ischemia manifested in the retina after 24 h: development of stable retinal edema, decrease in the b/a index (ratio of the electroretinogram b and a-wave amplitudes) to 1-2 units, pronounced disorders in the retinal microcirculation system, leading to ischemia of the inner layers of the retina. The proposed model is convenient for studies of the development of acute retinal ischemia, is easily realized, and reproduces some acute ischemic diseases of the retina.
    No preview · Article · Jul 2008 · Bulletin of Experimental Biology and Medicine
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: We used animal models to study connection between oxidating system and sphingomyelin signaling cascade, because this models are more close related to people disease. Activation of n-sphingomyelinase (n-SMase) in mice liver and brain is coincided in time with increased level of peroxide products (conjugated dienes) after injection of tumor necrosis factor alpha (TNF-alpha). We found that ceramide can induce peroxide oxidation and lead to accumulation of TNF-alpha in animal organs. Nitric oxide (NO) donors (S-nitrosoglutathione and dinitrosyl iron complex) reversibly inhibited activity of n-SMase and decreased level of lipid peroxidation products. This data proposed that both SMase and messengers of oxidative systems could be targets for NO-derived oxidants.
    Full-text · Article · Nov 2005 · FEBS Letters
  • A. E. Bugrova · L. B. Dudnik · A. V. Alessenko

    No preview · Article · Apr 2005 · European Neuropsychopharmacology
  • Source
    AV Alessenko · AE Bugrova · L B Dudnik
    [Show abstract] [Hide abstract]
    ABSTRACT: Alzheimer's disease (AD) is characterized by progressive decline in cognition, memory and intellect. It has been hypothesized that amyloid-beta peptide (A-beta) may have a prominent role in neurodegeneration. Oxidative mechanisms have been implicated in this pathway. There is substantial evidence that inflammatory mechanisms, induced by tumour necrosis factor alpha (TNF-alpha), are also involved in AD. TNF-alpha activates receptors linked to multiple effector systems, including a sphingomyelin pathway and peroxide oxidation. We have determined the changes of neutral sphingomyelinase activity, sphingomyelin and ceramide contents, and the level of lipid peroxide products (conjugated dienes), in the cerebral cortex, hippocampus and cerebellum of rats within 3 h and 7 days of intracerebral injection of A-beta and TNF-alpha. A single injection of A-beta and TNF-alpha has been shown to increase the level of peroxide products in the hippocampus and cerebral cortex within 3 h and 7 days. Sphingomyelinase activity and ceramide levels have been found to increase 7 days after A-beta administration. We found that activation of the sphingomyelin pathway lies downstream from the oxidative stress.
    Full-text · Article · Mar 2004 · Biochemical Society Transactions
  • [Show abstract] [Hide abstract]
    ABSTRACT: The influence of separate and combined intracerebroventricular injection of beta-amyloid peptide (beta-A), in a form of febrile and globular aggregates (3nM per rat), and tumor necrosis factor a (TNF-alpha) (2 mcg per rat) on sphingomyelin cycle (sphingomyelinase activity, sphingomyelin and ceramide content) and on oxidative stress development in the hippocampus, cortex and cerebellum of Wistar rats was studied. The indices were detected 3 h and 7 days after the injection. In case of a separate injection, sphingomyeninase activity decreased in 3h. But enzyme activity exceeded the control values in the hippocampus after 7 days, when morphological lesions were first ever detected. In combined action of the polypeptides, there was enzyme activity normalization. In all types of injections, changes of sphingomyeninase activity were minimal in the cortex and cerebellum. Content of ceramide, apoptosis inductor, increased in the hippocampus 7 days after separate injection. Induction of sphingomyelin cycle, resulting in accumulation of a proapoptotic agent, ceramide, may be regarded as a new Alzheimer disease mechanism. Peroxidation products (dienic conjugates and diene ketones) accumulate early after surgery (3h later) and their content dramatically increase 7 days after separate beta-A and TNF-alpha injection. Combined injection of beta-A and cytokine in a small dose leads to beta-A toxic effect withdrawal that may indicate a TNF-alpha protective effect in the dose used.
    No preview · Article · Feb 2004 · Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova / Ministerstvo zdravookhraneniia i meditsinskoi promyshlennosti Rossiiskoi Federatsii, Vserossiiskoe obshchestvo nevrologov [i] Vserossiiskoe obshchestvo psikhiatrov

Publication Stats

77 Citations
24.74 Total Impact Points

Top co-authors View all


  • 2005-2012
    • Russian Academy of Sciences
      • Institute of Biochemical Physics
      Moskva, Moscow, Russia