Mohamed Gorcii

University of Monastir, Al Munastīr, Al Munastīr, Tunisia

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Publications (26)31.32 Total impact


  • No preview · Article · Jan 2015 · Journal of Bacteriology & Parasitology

  • No preview · Article · Dec 2014 · Annales de Dermatologie et de Vénéréologie
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    ABSTRACT: Alternariosis is a fungal infection that is usually described in immunocompromised patients. We report a case of cutaneous alternariosis in a renal transplant recipient caused by Alternaria tenuissima. The diagnosis was supported by histopathologic (ie, yeastlike cells, filamentous structures) and mycologic findings from a cutaneous biopsy. Cutaneous lesions regressed 1 month following a decrease in the dosage of immunosuppressive therapy. The patient also was treated with intravenous amphotericin B followed by oral fluconazole without improvement. Cryotherapy remarkably accelerated healing of the lesions.
    No preview · Article · May 2014
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    ABSTRACT: Visceral leishmaniasis (VL) has been endemic in northern Tunisia and has occurred sporadically in the center of Tunisia. Recently, there have been several cases from areas known to be free of VL. We report in this work all human and canine cases of VL recorded between 2003 and 2011 and an entomological study of phlebotomine fauna in a previously non-endemic region. Sixty-three cases of VL were diagnosed and identified as L. infantum using several different methods. Eight species of 179 sand flies were caught and identified by both morphological and molecular methods. Two genera were present, Phlebotomus and Sergentomya, with an abundance of the subgenus Phlebotomus (Larrousius) spp., a classic vector of VL in Tunisia. Moreover, Leishmania DNA was detected in seven unfed Phlebotomus pernicousus and L. infantum was identified in three of them. This result confirms the establishment of a transmission cycle of VL in the studied region by the coexistence of infected vectors with infected hosts.
    Full-text · Article · Jun 2013 · Journal of Vector Ecology
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    ABSTRACT: Objective Studying the epidemiological variations of visceral leishmaniasis in Tunisia and proving the importance of parasitological investigations to raise the diagnosis.Patients and methodsSix patients hospitalised during the period between January 1998 and January 2009 at Fattouma Bourguiba Teaching Hospital in Monastir, five men and an only one woman, aged from 26 to 70 years old, originating from the central and eastern regions of the country. Epidemiological, clinical, biological and therapeutic data were obtained from the patient's medical files.ResultsThe major clinical symptoms were fever, weakness and spleen enlargement. Biological data revealed the presence of anaemia in every case and leucopoenia associated or not associated with thrombopenia in four cases. The diagnosis of visceral leishmaniasis was confirmed by the identification of the parasite in the blood or in the bone marrow. All patients were treated with two courses of antimoniate of meglumine separated by a 6-week interval. The outcome was positive and the patients were cured.Conclusion Visceral leishmaniasis is increasing among adults in Tunisia. Moreover, it is spreading outside its epidemiological area in the north to reach the central and southern regions. It should be raised when fever and spleen enlargement occur. Biological data are hardly specific. Diagnosis is based on finding the parasite in human fluids, mainly by molecular techniques. The rapid establishment of a specific treatment is vital.
    No preview · Article · Apr 2013 · Pathologie Biologie
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    ABSTRACT: Infections are a major determinant of outcome in kidney transplantation. Opportunistic pathogens are common in kidney recipients and several organs can be affected. Central nervous system infection in transplant recipients is a medical emergency. There is limited information in the literature concerning post-transplantation cryptococcal infection. Deafness and blindness are not classic findings. We report a case of meningocerebral cryptococcosis complicated by deafness and blindness after kidney transplantation. Physicians need to consider the possibility of Cryptococcus neoformans when symptoms persist despite empiric antimicrobial therapy.
    No preview · Article · Jan 2013 · Saudi journal of kidney diseases and transplantation: an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia
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    ABSTRACT: Over a period of ten years, a series of 694 Leishmania strains from Tunisian leishmaniasis foci were isolated and identified by isoenzymatic analysis. Strains were obtained from human cutaneous and visceral leishmaniasis in immunocompetent subjects, visceral leishmaniasis in imunocompromised individuals and from dogs with visceral leishmaniasis. Two classically dermotropic species, Leishmania (L.) major and Leishmania killicki were found. L. major with the single zymodeme MON-25 was the most isolated in cutaneous leishmaniasis foci of the Centre and South of Tunisia with a recent northern extension. L. killicki zymodeme MON-8 was sporadically found both in its classical microfocus of Tataouine in southeastern Tunisia as well as in some new foci in Southwestern, Central and Northern Tunisia. Leishmania infantum with its three zymodemes MON-1, MON-24 and MON-80 was isolated from both visceral and cutaneous human cases. The majority of L. infantum strains were found in the Northern part of the country; however, some strains were reported for the first time in the Southern part. L. infantum MON-1 was the only zymodeme isolated from canine leishmaniasis.
    Full-text · Article · Aug 2012 · Acta tropica
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    ABSTRACT: ABSTRACT Knowledge of the host-feeding pattern of blood-sucking insects helps to understand the epidemiology of a vector-born disease. A set of primers was used to selectively amplify segment of vertebratesÕ prepronociceptin gene from abdomens of engorged sand ßies. Vertebrate DNA was successfully ampliÞed in 65% of blood-fed phlebotomines assayed. Direct sequencing and comparison of resultant sequences with sequences in GenBank, using Basic Local Alignment Search Tool, led to the speciÞc identiÞcation of the host in 100% of the cases. In total, 249 blood-fed females belonging to Þve different sand ßies species were captured thanks to Centers for Disease Control and Prevention light traps and sticky papers in different areas of Tunisia between 2007 and 2009. Bloodmeal origin was determined for 146 blood-fed midges: Phlebotomus sergenti Parrot sampled fed only on Ovis aries and Equus caballus, while bloodmeal origin of P. perniciosus Newstead, P. longicuspis Nitzulescu, and P. papatasi (Scopoli) was diversiÞed. We found that midges were fed mainly on Homos sapiens (n = 37; 22.69%), Bos taurus (n = 11; 6.74%), Mus musculus (n = 2; 1.22%), Capra hircus (n = 4; 2.45%), Camelus dromedarius (n = 3; 1.84%), Ovis aries (n =98; 60.12%), Equus caballus (n = 3; 1.84%), Felis catus (n = 1; 0.6%), Oryctolagus cuniculus (n = 3; 1.84%), and Rattus norvegicus (n = 1; 0.6%). In this study, interestingly, we found for the Þrst time that Mus musculus DNA was found in one female of S. minuta (Rondani) specie and question about its possible vectorial role is opened.
    Full-text · Article · Aug 2012
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    ABSTRACT: Our aim was to study the distribution and the fertility of the hydatid cysts in function of the age and the sex of patients and to identify the strain(s) responsible(s) of the children hydatidosis. We have analyzed a total of 241 cysts coming from 195 children aged 2 to 16 years operated in the CHU F. Bourguiba of Monastir during the period from November 1999 to December 2009. For each cyst, the localization and the fertility of the métacestode as well as age, sex and origin of the patient are listed. Identification of strains was carried out by PCR/RFLP and has targeted the ribosomal gene ITS1. The lung was the primary localization of cyst (61.8%) followed by the liver (34.85%). The greatest number of cases is observed in the age groups 4-9 years (138 cases) where children's infection is more frequent in the male than in the female sex. The fertility of the cyst was independent of its site or its size and no incidence of age of children was detected. The G1 sheep strain is responsible for the contamination of children. The cystic echinococcosis described as a young adult disease may actually observed at any age and remains a serious problem of public health in Tunisia.
    No preview · Article · Jun 2012 · Pathologie Biologie
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    ABSTRACT: The aim of this study is to assess the usefulness of a simple, low-cost method for the detection and species identification of Leishmania isolated by in vitro culture or detected directly from clinical samples. A total of 110 samples were used in this study. Among these, 21 were human and canine peripheral bloods, 63 skin lesion material samples, eight reference strains and 18 Leishmania culture. Detection of Leishmania DNA with PCR using primers designed to amplify the internal transcribed spacer 1 (ITS1) region of the rRNA gene proved sufficiently sensitive at the level of 0.1 parasites per PCR reaction. Furthermore, followed by single-strand conformational polymorphism (SSCP), the PCR-ITS1 allowed the species identification of Leishmania. The inter-specific polymorphism of Leishmania was first validated on reference strains, and then this method was applied on clinical samples and culture. Typing identified all human and canine visceral leishmaniasis samples (21 samples) as L. infantum, 95.23% of the cutaneous leishmaniasis samples as L. major and 3.17% as L. killicki and 1.58% as L. infantum. A scheme of the PCR diagnosis procedure for the detection and identification of Leishmania parasites is proposed in this study.
    Full-text · Article · Feb 2012 · Pathologie Biologie
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    ABSTRACT: Metlaoui district in the South-west of Tunisia is a classical focus of cutaneous leishmaniasis (CL) due to Leishmania major. Since 2005, a single case of CL due to L. killicki has been reported. We report twenty four human cases due to this parasite, affecting men and women from 2 to 70 years old. Leishmania killicki have been typed using molecular techniques: polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) and gene sequencing. Four strains from patients have been successfully cultured on NNN medium and isoenzymatically typed as L. killicki MON-8. Our results strongly suggests that Metlaoui is a new L. killicki focus with a stable transmission cycle. Sand flies fauna in the same focus was also studied. 1400 Phlebotomine sand flies (785 males/615 females) have been caught during an entomological survey. Leishmania major DNA has been found in one P. papatasi female, the most abundant species, whereas L. killicki DNA has been found in one Phlebotomus sergenti female emphasizing the probable role of this species as vector of this zoonotic parasite.
    Full-text · Article · Jan 2012 · Acta tropica
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    ABSTRACT: Leishmania killicki was originally described in 1980 in southeast Tunisia. It was also recently reported in Lybia and Algeria. Nevertheless, neither vector nor reservoirs of this parasite are known. The identification of the vector and the animal reservoir host of L. killicki is critical for the establishment of an efficient control strategy. blood, popliteal lymph node, spleen, bone marrow, liver and skin were collected from 50 rodents in 2009 in south western Tunisia. Samples were smeared onto glass slides, cultured on NNN medium and tested by polymerase chain reaction for Leishmania detection. Parasites were detected by PCR from 10 Psammomys obesus and from two Ctenodactylus gundi. Parasite identification was performed simultaneously by internal transcribed spacer 1 PCR-RFLP and by PCR sequencing. Both Leishmania major and Leishmania killicki were identified from infected Psammomys and Ctenodactylus gundi respectively. This is the first report of Leishmania killicki identified from Ctenodactylus gundi in Tunisia. This result supports the assumption that C. gundi is a potential reservoir for Leishmania killicki.
    Full-text · Article · Aug 2011 · Parasites & Vectors
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    ABSTRACT: Topoisomerase II gene of Leishmania genus was used to develop a molecular tool for detection and species differentiation of Leishmania from clinical samples. Identification was achieved by a polymerase chain reaction followed by digestion with 2 restriction endonucleases BstU1 and Taq1. Despite the relatively low sensitivity, it is able to differentiate between 3 complexes responsible for cutaneous leishmaniasis.
    Full-text · Article · Oct 2010 · Diagnostic microbiology and infectious disease
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    ABSTRACT: The prevalence of onychomycosis due to non-dermatophytic moulds have been increased during the last few years. The reported pathogenic fungi are multiple and their list is continuously increasing. We report a case of onychomycosis in the left great toe due to Aspergillus sclerotiorum in a 45-year-old woman without any pathologic history. Clinical aspect was a distolateral onychomycosis with subungual hyperkertosis, white coloration of the nail and periungual inflammation. This onychomycosis was improved by local treatment with amorolfine solution. Onychomycoses due to Aspergillus spp. are more frequent in toenails, mainly in the great toe. They affect especially adult woman. According to many authors, clinical feature of the onychomycosis can suggest its aspergillar nature. Aspergillar onychomycoses can be successfully treated by local or systemic antifungal drugs.
    No preview · Article · Jun 2010 · Journal de Mycologie Médicale/Journal of Medical Mycology
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    ABSTRACT: Fungus ball is rarely associated with acute renal failure (ARF). We report the case of a 65-year-old diabetic patient who has been admitted for a right kidney infection. Urinary exam showed a leucocyturia with a negative urine culture. Ultrasonography showed pelvicaliceal dilation. Nephroscopy revealed a fungal material in ureter. Culture of this material was positive for Candida tropicalis. After endoscopic and antifongic therapy, the serum creatinin decreased from 336μmol/L to 150μmol/L, indicating the good evolution of his renal function.
    No preview · Article · Jun 2010 · Néphrologie & Thérapeutique
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    ABSTRACT: Fungus ball is rarely associated with acute renal failure (ARF). We report the case of a 65-year-old diabetic patient who has been admitted for a right kidney infection. Urinary exam showed a leucocyturia with a negative urine culture. Ultrasonography showed pelvicaliceal dilation. Nephroscopy revealed a fungal material in ureter. Culture of this material was positive for Candida tropicalis. After endoscopic and antifongic therapy, the serum creatinin decreased from 336 micromol/L to 150 micromol/L, indicating the good evolution of his renal function.
    No preview · Article · Jun 2010 · Néphrologie & Thérapeutique
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    ABSTRACT: Introduction The term Tinea Faciei (TF) designates a superficial dermatophyte infection of the glabrous skin of the face, characterized by clinical polymorphism. The aim of the present study is to assess epidemiologic, clinical, mycological, and therapeutic characteristics of TF in a Tunisian dermatology department. Patients and methods This retrospective study examined the records of cases of TF observed in our dermatology department over the past 5 years (2003–2007). Results Fourteen patients with TF were seen during the study period. The sex ratio (5 male/9 female) was 0.56. The average age of these 4 children and 10 adults was 22 years. While the clinical form was typical of TF in 10 cases, 4 patients had a clinical form modified by topical steroids (Tinea incognito). Direct microscopic examination showed hyphal elements in 12 cases, and the mycological culture was positive for 11. Five patients had Trichophyton Violaceum, and 4 T. Mentagrophytes. Discussion TF is frequently misdiagnosed because of its variable appearance. Atypical forms known as tinea incognito are observed after modification of the dermatophyte infections by corticosteroid treatment. Our study included 4 cases of Tinea incognito. T. Mentagrophytes and T. Rubrum are the agents reported most frequently. Unusually, T. Violaceum was found most frequently in our series.
    No preview · Article · Sep 2009 · La Presse Médicale
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    ABSTRACT: The term Tinea Faciei (TF) designates a superficial dermatophyte infection of the glabrous skin of the face, characterized by clinical polymorphism. The aim of the present study is to assess epidemiologic, clinical, mycological, and therapeutic characteristics of TF in a Tunisian dermatology department. This retrospective study examined the records of cases of TF observed in our dermatology department over the past 5 years (2003-2007). Fourteen patients with TF were seen during the study period. The sex ratio (5 male/9 female) was 0.56. The average age of these 4 children and 10 adults was 22 years. While the clinical form was typical of TF in 10 cases, 4 patients had a clinical form modified by topical steroids (Tinea incognito). Direct microscopic examination showed hyphal elements in 12 cases, and the mycological culture was positive for 11. Five patients had Trichophyton Violaceum, and 4 T. Mentagrophytes. TF is frequently misdiagnosed because of its variable appearance. Atypical forms known as tinea incognito are observed after modification of the dermatophyte infections by corticosteroid treatment. Our study included 4 cases of Tinea incognito. T. Mentagrophytes and T. Rubrum are the agents reported most frequently. Unusually, T. Violaceum was found most frequently in our series.
    No preview · Article · Mar 2009 · La Presse Médicale
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    ABSTRACT: The aim of this study was to assess the use of parasitological, serological and molecular methods for the detection of Leishmania infection in blood of 67 dogs and to investigate the prevalence of canine leishmaniasis (CanL) in Kairouan (central Tunisia), an area known to be of reduced endemicity and has not been studied since 1973. Veterinarians clinically examined all dogs, and the titer of anti-Leishmania antibodies was determined by indirect immune-fluorescence antibody test. The presence of Leishmania was performed by PCR and in vitro culture. IFAT was positive in 12% of dogs and promastigote form of the parasite was isolated by in vitro culture from only 4.5% of them. However, DNA of Leishmania was detected by PCR in 20.9% of dogs. PCR was more sensitive than IFAT (p = 0.004) and in vitro culture (p < 10(-5)). A prevalence of 21% was found in Kairouan, which is significant high (p < 10(-3)) when compared to that of thirty years ago. This state is in correlation with the increase in other Mediterranean countries. Furthermore, 50% of positive dogs were asymptomatic. Preventive measures must be taken against these dogs as for symptomatic ones since their role in the transmission of the infection to vectors has been proven.
    Full-text · Article · Mar 2009 · Parasite
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    ABSTRACT: We synthesized recombinant Echinococcus granulosus protoscolex recP29 antigen to be preliminarily assessed by ELISA and immunoblotting. RecP29-serology was carried out on 54 young patients with cystic echinococcosis (CE). Patients were classified into either cured (CCE) (n=40) or non-cured (NCCE) (n=14) CE patients. RecP29 ELISA showed a gradual decrease of antibody concentrations in all CCE cases that were initially (before treatment) seropositive to this antigen (25 out of 40) or that seroconverted following treatment. A complete seronegativity was reached within 3 years post-surgery in all of these cases. Conventional HCF ELISA yielded seronegativity in only 10% of initially recP29-seropositive CCE patients (P=0.086). Likewise, recP29 immunoblotting yielded seronegativity in 93% of 29 out of 40 initially recP29-immunoblot-positive CCE patients after 3 years follow-up, compared with 72% in the HCF immunoblotting (P=0.060). Eleven out of 14 NCCE patients were initially positive by recP29 ELISA, and 10 out of these maintained a marked anti-recP29 antibody reactivity until the endpoint of the follow-up period. All 14 NCCE cases were initially seropositive by recP29 immunoblotting, and 13 cases remained seropositive until the end of the study. Thus, recombinant P29 protein appears prognostically useful for monitoring those post-surgical CE cases with an initial seropositivity to this marker.
    No preview · Article · Dec 2008 · Transactions of the Royal Society of Tropical Medicine and Hygiene

Publication Stats

220 Citations
31.32 Total Impact Points

Institutions

  • 2008-2014
    • University of Monastir
      Al Munastīr, Al Munastīr, Tunisia
  • 2013
    • Hôpital Universitaire Fattouma Bourguiba
      Al Munastīr, Al Munastīr, Tunisia