Changfa Lin

Fudan University, Shanghai, Shanghai Shi, China

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Publications (3)6.81 Total impact

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    ABSTRACT: The ATP-dependent Lon protease is a highly conserved enzyme that is present in archeae, eubacteria, and eukaryotes, and plays an important role in intracellular protein degradation. We have isolated a Lon protease gene, OsLon1, from Oryza sativa. The cDNA contained a 2,655 bp ORF. Comparative analysis showed that OsLon1 shared significant similarity with the previously reported Lon proteases from maize, Arabidopsis, human, and bacteria. Tissue expression pattern analysis revealed that OsLon1 was highly expressed in young leaves, mature leaves, and leaf sheaths but only weakly in young roots, mature roots, and young panicles. The OsLon1 gene was successfully expressed in E. coli and the detected protein size, about 120 kDa, matched the expected molecular mass of the His-tagged OsLon1 protein.
    No preview · Article · Jul 2006 · Biotechnology Letters
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    ABSTRACT: Members in the TERMINAL FLOWER 1 (TFL1)/CENTRORADIALIS (CEN) gene family play important roles in controlling inflorescence architecture and phase transition in several different plant species. To investigate the biological functions of TFL1/CEN homologs in rice, we isolated and analyzed four rice TFL1/CEN-like genes, Oscen1–4. We showed that these genes have weak basal expression throughout rice development, and most have distinct expression patterns mainly in the secondary meristems as revealed by mRNA in situ hybridization analyses. The 35S::Oscen1 and 35S::Oscen2 transgenic rice plants exhibit increased numbers of internodes, shortened length and altered radial patterns in the elongated internodes, as well as delayed heading and abnormal panicle architecture. These findings suggest that the TFL1/CEN-like genes in rice play distinct roles in regulating the development of basic structures by stimulating the activities of secondary meristems in the uppermost phytomers.
    No preview · Article · Jun 2005 · Plant Science
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    ABSTRACT: Using Rapid Amplification of cDNA ends (RACE) technique, the full-length cDNA encoding a NaCl-induced fructose-1, 6-diphosphate aldolase (DsALDP) was obtained. It was shown that the DsALDP had a relatively high homology (66%-73%) to chloroplast fructose-1, 6-diphosphate aldolase (AldP) in many plants according to their amino acid sequences. The phylogenetic analysis further confirmed that AldP in alga is the nearest to DsALDP. As to its expression pattern, DsALDP was de novo synthesized by NaCl induction. Its expression level was significantly changed with inducing time. After the selected DsALDP cDNA subcloned into a binary vector pBI121, the new construct was introduced into tobacco by Agrobacterium tumefaciens. The results of Southern blot and RT-PCR analysis of four transgenic T1 plants indicated that DsALDP was integrated into genome of these transgenic plants and effectively expressed. Aldolase activities have been detected in T1-1, T1-2 and T1-3 plants by bioassay under 100-200 mmol/L NaCl. It was also observed that proline contents in them were differentially increased.
    No preview · Article · Feb 2003 · Science in China Series C Life Sciences