[Show abstract][Hide abstract] ABSTRACT: Nasal-type NK/T cell lymphoma (NK/TCL) is an increasingly recognized disease entity of aggressive clinical behavior. The objective of this study was to investigate the correlation between the clinical features of NK/TCL and patient prognosis. Immunohistochemical detection of Epstein-Barr virus (EBV)-associated latent membrane protein (LMP)-1, Cyclin D1 and Bcl-2 protein expression, as well as TUNEL staining, were performed. Of the 58 cases studied, 47 (81%) displayed positive expression of LMP-1 protein, which was associated with the presence of B symptoms and patient prognosis. In addition, high expression of Cyclin D1 and Bcl-2 protein was observed to correlate with poor prognosis and decreased survival. More importantly, LMP-1 and Cyclin D1 appeared to be co-expressed. This, along with other parameters such as clinical stage at diagnosis and enlarged lymph nodes, could be used as an independent prognostic factor for clinical outcome, as the survival rate of NK/TCL patients was closely associated with LMP-1/Cyclin D1 co-expression, advanced clinical stage and lymph node infiltration.
Preview · Article · May 2008 · Molecular Medicine Reports
[Show abstract][Hide abstract] ABSTRACT: Vasculogenic mimicry (VM) is the formation of fluid-conducting channels by highly invasive and genetically dysregulated tumor cells. In this study, we collected specimens of 84 human gastrointestinal stromal tumors (GISTs) along with clinicopathologic data and another 42 GISTs with fresh tissue that was used for gelatin zymography. VM was found in 21 of the 84 GISTs using CD31/periodic acid-Schiff double staining and CD117 and CD31 immunohistochemical staining. There was a significant difference in the VM-positive rate between the lesions with a mitotic rate > or =5/50 high-power fields and those with a lower mitotic rate (P = .000) and between the cases with and without liver metastasis (P = .008). There was a significant difference in the VM-positive rate between the high-risk group (5.9%) and the very low/low-risk group (12.5%) (P = .010) or the intermediate-risk group (39.5%) (P = .020). Kaplan-Meier survival analysis showed VM indicated a poor prognosis (P = .0000). Cox proportional hazards model indicated that the presence of VM, tumor size 10 cm or greater, and hemorrhage were independent predictors of a poor prognosis (P = .000, .005, .032, respectively). The staining indexes of matrix metalloproteinase (MMP)-2 and MMP-9 were higher in the VM-positive than in the VM-negative group (P = .024 and .037, respectively). Gelatin zymography showed that the activity of MMP-2 and MMP-9 was significantly higher in the VM-positive lesions (P = .013 and .033, respectively). We conclude that VM in GISTs is an unfavorable prognostic sign and that patients with VM-positive tumors are prone to suffer liver metastasis. Both MMP-2 and MMP-9 play an important role in VM formation in GISTs.