Yoko Yakabe

National Institute of Livestock and Grassland Science, Ibaragi, Ōsaka, Japan

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Publications (9)12.32 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Ketone bodies such as β-hydroxybutyrate and acetoacetate have physiological functions in addition to being used as an energy source. In order to assess the effect of elevated ketogenesis on blood lipid profiles and redox status, a ketogenic diet (KD), a high-fat, low-carbohydrate, and low-protein diet, was fed to chicken for 4 weeks. Plasma β-hydroxybutyrate, but not acetoacetate, concentrations were significantly increased by KD feeding for 2 and 4 weeks. The KD also induced elevation of plasma non-esterified fatty acid (NEFA) and total cholesterol concentrations, whereas plasma triglyceride concentration was decreased. Plasma total antioxidant activity in chicken with ketosis induced by KD was lower than that of the control. However, the level of plasma TBARs, an oxidative stress marker, was also reduced by KD feeding. A reduction of energy intake was observed in chickens fed the KD; therefore, the effect of a restricted diet (RD) was also investigated. Plasma β-hydroxybutyrate concentration, lipid (total cholesterol and NEFA) concentration, and redox status were not affected by RD feeding. These data suggest that a high-fat, low-arbohydrate, and low-protein diet induces ketosis by elevating blood β-hydroxybutyrate concentration in chicken. Under conditions of ketosis induced by the KD, total antioxidant capacity was reduced along with a modulation of the blood lipid profile in chicken.
    No preview · Article · Jul 2013 · The Journal of Poultry Science
  • Hiroyuki Abe · Yoko Yakabe · Masahiro Amari

    No preview · Article · Jan 2013 · Nihon Chikusan Gakkaiho
  • M Yamazaki · H Ohtsu · Y Yakabe · M Kishima · H Abe
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    ABSTRACT: 1. The aim of this work was to select lactic acid bacteria (LAB) strains from chicks and hens of egg-laying strains for potential use to control Salmonellae. 2. Nineteen LAB strains obtained from culture collections, and 24 strains isolated from excreta of laying hens and chicks, were evaluated for inhibitory capacities against two Salmonella serotypes using a "Spot-the-lawn" technique and other in vitro properties that could be predictive of antimicrobial activity. 3. The size of the inhibition zone differed slightly between Salmonella serotypes, however, the mean size of the Salmonella inhibition zone differed greatly among the LAB strains. Lactobacillus salivarius, L. plantarum, L. rhamnosus and L. reuteri exhibited powerful inhibitory effects to each Salmonella strain. 4. The result of the acid tolerance test showed that all L. salivarius, L. kitasatonis strains and each of L. ingluviei cannot survive in a low pH environment. In the bile acid tolerance assay, growth was inhibited in all strains, except L. kitasatonis HE4, and a large inhibition was observed in most of the L. salivarius and L. crispatus strains. 5. The results demonstrate that some LAB of poultry origin were able to inhibit the growth of Salmonella and survive simulated passage through the gastrointestinal tract. The selected LAB could act in the lower gastrointestinal tract to prevent salmonellosis in poultry.
    No preview · Article · Apr 2012 · British Poultry Science
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    ABSTRACT: Experiments were conducted to investigate the effect of the Bacillus subtilis var. natto fermented soybean (FS) on growth performance, microbial activity in the caeca and cytokine gene expression in spleen of domestic meat type chickens. Chicks were fed commercial diets supplemented with 0 (control), 1 or 2% FS for 80 days from 0 to 80 days of age, and a fourth group of chicks were fed control diet from 0 to 27 days of age, and then fed a 2% FS diet from 28 to 80 days of age. Supplementation with FS did not decrease feed intake compared to chicks fed the control diet, and there were no significant differences in body weight gain and feed efficiency due to dietary treatment. FS supplementation had no effect oil carcass weight, breast and thigh meat composition, or abdominal fat deposition. There were no significant differences in pH. NH3-N concentration, or number of microorganisms in the cecum content in each group; however. total acetic acid concentration increased (P < 0.05) in chicks fed the 2% FS Supplemented diet from 0 to 80 days of age. Supplementation with FS did not affect IFN-gamma, IL-4 and IL-13 gene expression in spleens. It can be concluded from this study that supplementing FS to meat type chicken diets has no deleterious effect on the growth performance. and has beneficial effects on intestinal microbial activity, as observed by increases in VFA concentration.
    No preview · Article · Apr 2009 · The Journal of Poultry Science
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    Kazuki Nakashima · Yoko Yakabe
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    ABSTRACT: In skeletal muscle, AMP-activated protein kinase (AMPK) is a metabolic master switch regulating glucose and lipid metabolism. Recently, AMPK has been implicated in the control of protein synthesis in skeletal muscle, but the effect of AMPK activation on myofibrillar protein degradation has yet to be elucidated. The present study was designed to examine the effect of 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside (AICAR)-induced AMPK signaling on effector mechanisms of myofibrillar protein degradation and the expression of atrophy-related genes (atrogin-1/MAFbx, MuRF1, proteasome C2 subunit, calpains, cathepsin B, and caspase-3) in C2C12 myotubes. AICAR stimulated myofibrillar protein degradation (as measured by N(tau)-methylhistidine release), while also increasing the levels of atrogin-1/MAFbx and MuRF1 mRNA, but the expression of other atrophy-related genes was not enhanced by AICAR treatment in C2C12 myotubes. AICAR also stimulated the level of FOXO transcription factors mRNA and protein in C2C12 myotubes. These results indicate that activation of AMPK stimulates myofibrillar protein degradation through the expression of atrogin-1/MAFbx and MuRF1 by increasing FOXO transcription factors in skeletal muscles.
    Preview · Article · Feb 2008 · Bioscience Biotechnology and Biochemistry
  • K Nakashima · Y Yakabe · A Ishida · M Yamazaki · H Abe
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    ABSTRACT: We previously reported that L-leucine suppresses myofibrillar proteolysis in chick skeletal muscles. In the current study, we compared the effects of L- and D-enantiomers of leucine on myofibrillar proteolysis in skeletal muscle of chicks. We also assessed whether leucine itself or its metabolite, alpha-ketoisocaproate (alpha-KIC), mediates the effects of leucine. Food-deprived (24 h) chicks were orally administered 225 mg/100 g body weight L-leucine, D-leucine or alpha-KIC and were sacrificed after 2 h. L-Leucine administration had an obvious inhibitory effect on myofibrillar proteolysis (plasma N(tau)-methylhistidine concentration) in chicks while D-leucine and alpha-KIC were much more effective. We also examined the expression of the proteolytic-related genes (ubiquitin, proteasome, m-calpain and cathepsin B) by real-time PCR of cDNA in chick skeletal muscles. Ubiquitin mRNA expression was decreased by D-leucine and alpha-KIC but not L-leucine. Proteasome and m-calpain mRNA expressions as well as cathepsin B mRNA expression were likewise decreased by L-leucine, D-leucine and alpha-KIC. These results indicate that D-leucine and alpha-KIC suppress proteolytic-related genes, resulting in an decrease in myofibrillar proteolysis while L-leucine is much less effective in skeletal muscle of chicks, may be explain by conversion of D-leucine to alpha-KIC.
    No preview · Article · Oct 2007 · Amino Acids
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    ABSTRACT: We examined the effects of orally administered glycine on myofibrillar proteolysis in food-deprived chicks. Food-deprived (24 h) chicks were orally administered 57, 113, and 225 mg glycine/100 g body weight and killed after 2 h. The plasma N(tau)-methylhistidine concentration, used as myofibrillar proteolysis, was decreased by glycine. We also examined the expression of proteolytic-related genes by real-time PCR of cDNA from chick skeletal muscles. The mRNA expression of atrogin-1/MAFbx, proteasome C2 subunit, m-calpain large subunit, and cathepsin B was decreased by glycine in a dose-dependent manner. The plasma corticosterone concentration was also decreased by glycine, but the plasma insulin concentration was unaffected. These results indicate that orally administered glycine suppresses myofibrillar proteolysis and expression of proteolytic-related genes of skeletal muscle by decreasing the plasma corticosterone concentration in chicks.
    No preview · Article · Aug 2007 · Amino Acids
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    ABSTRACT: This experiment was conducted to study the effects of fasting and refeeding on expression of the atrogin-1 and Akt/FOXO signaling pathway in skeletal muscle of chicks. Chicks were fasted for 24 h and refed for 2 h. Atrogin-1 mRNA expression was increased by fasting, and their increment was reduced by refeeding. Phosphorylations of Akt and FOXO1 were not decreased by fasting, but, they were increased by refeeding. These results indicate that refeeding stimulates phosphorylation of Akt/FOXO, resulting in a decrease in atrogin-1 expression in skeletal muscle of chicks.
    Preview · Article · Dec 2006 · Bioscience Biotechnology and Biochemistry
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    ABSTRACT: We examined the effects of orally administrated amino acids on myfibrillar proteolysis in food-deprived chicks. Plasma N(tau)-methylhistidine concentration, as an index of myofibrillar proteolysis, was decreased by the administration of Glu, Gly, Ala, Leu, Ile, Ser, Thr, Met, Trp, Asn, Gln, Pro, Lys and Arg but not by Asp, Val, Phe, Tyr or His to chicks. Orally administrated Cys was fatal to chicks. These results indicate that oral Glu, Gly, Ala, Leu, Ile, Ser, Thr, Met, Trp, Asn, Gln, Pro, Lys and Arg administration suppressed myofibrillar proteolysis in chicks.
    No preview · Article · Sep 2006 · Bioscience Biotechnology and Biochemistry