Publications (31)5.66 Total impact


  • No preview · Article · Jul 2015 · Journal of Clinical and Diagnostic Research
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    ABSTRACT: Background: Suppressive doses of levothyroxine therapy are reported to reduce bone mineral density (BMD) in women. Data on bone changes in premenopausal hypothyroid women with replacement therapy are limited. Hence, this study was undertaken to evaluate bone changes in this group using bone markers and BMD. Materials and methods: A hospital-based case-control study including 75 premenopausal women aged 30-45 years was conducted. The subjects were categorized based on their thyroid function and history into three groups of 25 euthyroid, 25 newly diagnosed hypothyroid, and 25 hypothyroid women on 100-200 μg of levothyroxine for a minimum of 5 years. The bone changes were evaluated and compared among the groups biochemically by estimating their plasma osteocalcin and serum calcium and phosphorus and radiologically by measuring their BMD by quantitative ultrasonography. Statistical analysis was conducted by using analysis of variance, Tukey's test, and Pearson's correlation using IBM SPSS Statistics 20. Results: Levels of plasma osteocalcin, serum calcium, and serum phosphorus in patients on long-term levothyroxine therapy were significantly higher than those in newly diagnosed hypothyroid women and in the euthyroid group. BMD showed definite features of osteopenia (T-score: -2.26 ± 0.5) among the women in the treatment group, while it was well within the normal range in the newly diagnosed and euthyroid women. A significant correlation was found between the osteocalcin levels and T-score. Conclusion: Hypothyroid women on long-term levothyroxine therapy showed signs of increased bone turnover and increased resorptive changes, though not frank osteoporosis. Hence, it may be important to evaluate the bone status of patients on levothyroxine for >5 years.
    Full-text · Article · Mar 2015
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    ABSTRACT: Dyslipidemia triggers a sequel of metabolic derangements such as insulin resistance, hyperglycemia and oxidative stress via vicious cycle. Dyslipidemia is characterised by elevation of plasma cholesterol, triglycerides (TGs), or both, or a low level of high-density lipoprotein (HDL) which in turn can progress to atherosclerosis a forerunner for ischemic heart disease (IHD). Dyslipidemia is seen even in subclinical hypothyroid patients.
    No preview · Article · Sep 2014 · Journal of Clinical and Diagnostic Research
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    ABSTRACT: This case illustrates that serum ferritin level is markedly elevated in patients with autoimmune haemolytic anaemia. A 48 year old male with a history of spleenectomy done 16 years back, presented with complaints of fever, cough with expectoration and pain abdomen. Laboratory investigation demonstrated highly elevated level of serum ferritin of 10100 units.
    No preview · Article · Jul 2014
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    ABSTRACT: This case illustrates that D-dimer is elevated in patients with acute myeloid leukemia. A 21-year old lady presented with complaints of menorrhea and fever associated with swelling and bleeding from her gums. Routine laboratory investigations demonstrated an elevated D-dimer of 11.3mg/L. A diagnosis of acute myeloid leukemia was made. Peripheral smear showed presence of blast cells and decreased platelets. This case report highlights the mounting evidence that D-dimer is elevated in acute myeloid leukemia and could be useful as a prognostic marker.
    No preview · Article · Apr 2014
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    ABSTRACT: Levothyroxine (LT4) therapy has shown to have effects on bone metabolism though its deleterious effect on bone remodeling is debatable. This study was aimed at assessing the diagnostic utility of the bone remodeling marker C-terminal telopeptide (CTx) in detecting early bone loss. In this case-control study, 84 premenopausal women of 30-45 years of age were selected. Out of them, 28 were recently diagnosed of hypothyroidism (not on LT4), 28 were on LT4 replacement therapy (100-200 μg/day) for more than five years, and 28 had euthyroid. Plasma CTx levels were estimated. Bone mineral density (BMD) was measured by quantitative ultrasound (QUS) method. Pearson's coefficient of correlation and ANOVA were used for statistical analysis. CTx was most elevated in LT4-treated group (0.497 ± 0.209 ng/mL). It showed a significant negative correlation with T-score and Z-score of BMD values. In the treatment group of more than 150 μg/day, CTx showed significantly negative correlation with TSH (r = -0.462, P = 0.047). LT4 therapy induces bone loss in hypothyroid patients. CTx levels can measure such bone loss along with BMD. Regular monitoring of CTx with adjustment in LT4 doses may help delay osteoporosis induced by prolonged LT4 replacement therapy.
    Full-text · Article · Mar 2014 · Biomarker insights
  • Rajeshwari · B. D'Souza · V. D'Souza
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    ABSTRACT: Non prostatic tartarate resistant acid phosphatase(TRACP) is present in bone resorbing osteoclasts. Matrix degradation products together with TRACP are released from the osteoclasts into the blood circulation. Therefore this study is carried out to study TRACP as a marker for osteoporosis and bone malignancy. A total of 75 subjects were included in this study. They were divided into 3 groups. Group I included 25 diagnosed cases of malignant bone tumors between the age group 20 to 70 years of both sexes. Group II included 25 cases of osteoporosis and group III, the control group, included 25 healthy individuals of the same age groups and sexes. TRACP was estimated in the serum of all the subjects. TRACP levels were highly increased in both the osteoporosis and bone malignancy groups (p<0.001). Therefore TRACP can be considered as a marker for bone resorption in osteoporosis and bone malignancy.
    No preview · Article · Jan 2014 · International Journal of Pharma and Bio Sciences
  • R.P. Babu · A. Christy · B. D'Souza · V. D'Souza
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    ABSTRACT: The spectrum of anemias includes several varieties of subtypes and though each has distinctive characteristics, they seldom show overlapping features due to their specific etiology. This is a rare case of a 49 year old patient who presented with weakness and chronic fatigue with features of haemolytic anemia such as jaundice and elevated LDH levels, but on evaluation showed severe anemia with vitamin B12 deficiency thus having underlying megloblastic anemia.
    No preview · Article · Oct 2013
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    ABSTRACT: The aim of this study was to ascertain the effect of atorvastatin on oxidative stress parameters in dyslipidemic patients with or without diabetes. Advanced oxidation of protein products(AOPP), was measured as a marker for protein oxidation, malondialdehyde was studied as a marker of lipid peroxidation and glutathione (G-SH) was estimated to study the antioxidant potential of these patients. The results were compared with normal healthy control subjects. This study suggests that there is a significant increase in both AOPP and MDA in dyslipidemic, diabetic patients with or without atorvastatin. There is no significant change in antioxidant G-SH levels in these patients, compared to the control subjects. This study indicates that atorvastatin increases the oxidative stress.
    No preview · Article · Oct 2013 · International Journal of Pharma and Bio Sciences
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    Full-text · Dataset · Aug 2013
  • R. Babu · A. Christy · V. D'Souza · B. D'Souza
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    ABSTRACT: A case of a female patient who came with the history of fever with chills for two weeks duration and was extensively evaluated for the cause of fever. All other causes of fever were excluded. The patient had elevated level of Lactate dehydrogenase (LDH) upto 966IU/L. On CT and biopsy of the enlarged lymph nodes, patient was found to have hilar lymphadenopathy with reactive lymphadenitis, which could have been the cause for her symptoms. The cause of the elevated LDH remains to be evaluated.
    No preview · Article · Jul 2013
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    ABSTRACT: Objectives: The present study was conducted to evaluate the effect of a standardized hydroalcoholic root extract of Sala¬cia oblonga (SOE) on the Random Blood Glucose (RBG) levels, serum insulin, glycated haemoglobin (HbA1c) and the serum lipid profile in long standing, experimentally induced Diabetes Mellitus (DM) with glibenclamide (Glb) as the standard. Materials and methods: Streptozotocin (STZ) induced, dia-betic, Wistar rats of either sex were treated with two oral doses of SOE, 100 and 50mg/kg body wt /day, for a period of 16 weeks. The RBG was estimated at day-1 and at the end of the 16 weeks by using a glucometer. The fasting serum insulin was determined by an ELISA technique. The plasma HbA1c was evaluated by a Turbidimetric Inhibition Immunoassay (TINIA) and the lipid profile was estimated enzymatically. Results and analysis: A 45% decrease in the RBG was seen after the treatment with the higher dose of SOE, whereas a 44% decrease was observed with the lower dose as com¬pared to the diabetic control. Serum insulin was significantly increased (P<0.05) in all the treated groups as compared to the diabetic control. Plasma HbA1c was significantly decreased (P<0.05). The serum Triacyl Glycerol (TG) levels were signifi¬cantly decreased (P<0.05) in the treated rats as compared to the diabetic control. A significant increase in HDL-cholesterol (P<0.05) in the diabetic rats as a result of the 100mg/kg SOE treatment was a remarkable finding. Conclusion: SOE improves the glycaemic parameters in diabetic rats after a prolonged treatment. The serum TG levels were normalized on treatment. A higher dose of the extract could not alter the parameters significantly, except for HDL-C.
    No preview · Article · Dec 2012
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    ABSTRACT: Adult onset Still's disease (AOSD) is a systemic inflammatory disorder characterized by prolonged high grade fever, arthralgia, salmon color rashes. It is often complicated by a fatal condition called hemophagocytic syndrome. Serum Ferritin levels are extremely elevated in AOSD. We are reporting one such case, with ferritin level of more than 1 lac.
    No preview · Article · Oct 2012
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    ABSTRACT: Two known cases of diabetes mellitus, with plasmodium vivax malaria who had abnormally low HDLc(High density lipoprotein cholesterol) levels in the range of 2-5 mg/dl and abnormally high TC/HDLc ratio (Total cholesterol/HDLc) were observed during treatment in the hospital. The low HDLc levels were related to parasitaemia. Plasma triglyceride concentrations were moderately increased and plasma low density lipoprotein cholesterol (LDLc) concentrations decreased during the course of infection. The mechanisms underlying the selective and pronounced decline in HDLc concentration remain obscure, hence needing further evaluation in the direction.
    No preview · Article · Jul 2012
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    ABSTRACT: Background: Urbanization, rapid industrialization, increased vehicular traffic, and consequent increase in the use of petroleum fuels in India are constantly emitting lead along with other pollutants into the environment. Apart from atmospheric lead, this element is the most widely used in everyday life. Although infants and children are the most susceptible to the effects of lead, adults are also affected to varying degrees and it had ranked as one of the most serious environmental threats to human health. Hence, we must understand the benefits of preventing lead exposure as it reduces treatment costs, increases productivity in industry, and also reduces infant mortality. These are good enough reasons for a nation wide program to prevent lead poisoning. Objectives: In the view of elevated blood lead levels (BLL) in majority of the school children in the city of Mangalore, we aimed to identify the potential sources of lead in the environment which would have probably caused the elevated BLL. Materials and Methods: More than 600 readings were taken throughout the city of Mangalore using X-ray fluorimeter. Results: Our results showed that there were elevated levels of lead in the environment surrounding the battery repair shops, battery recyclers, automotive workshops, and tyre retreaders, but interestingly, the soil around the petrol bunks did not show elevated levels of lead. Among the paints, the yellow paint showed high levels of lead. Conclusion: Similar surveys would be useful elsewhere in India and in other developing countries in order to identify the potential sources of lead and to prevent lead poisoning.
    Full-text · Article · Jan 2012 · Indian Journal of Medical Sciences
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    ABSTRACT: Unlabelled: The serum acid phosphatase (ACP) activity and Hemoglobin (Hb) levels were measured in malaria patients and nonmalarial fever patients. The results were compared with normal healthy control subjects. ACP was significantly increased (P < 0.001) in all the malaria patients. ACP was significantly higher in Plasmodium falcifarum malaria and mixed malaria when compared to Plasmodium vivax malaria. Hb levels were significantly decreased in all the malaria patients which indicates that malaria parasite uses host erythrocyte Hb as a major nutrient source. There is negative correlation(r = -0.478) between ACP and Hb in malaria patients, which is highly significant. These results suggest that the measurement of ACP could be used as a marker for malaria. Electronic supplementary material: The online version of this article (doi:10.1007/s12291-011-0137-z) contains supplementary material, which is available to authorized users.
    Full-text · Article · Oct 2011 · Indian Journal of Clinical Biochemistry
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    ABSTRACT: Objective: The measurement of levels of transition metals and electrolytes is of considerable interest in the etiology of brain tumors. Material and Methods: Iron (Fe) and copper (Cu) and electrolytes such as sodium (Na) and potassium (K) levels were studied in 27 patients diagnosed with primary brain tumor and 25 normal healthy age and sex matched controls. Result: Significant decrease was observed in serum iron and sodium, serium copper and potassium were increased. Conclusion: Measurement of Serum level of sodium, Potasium, Iron and Copper level significance in the assement of primary brain tumor.
    No preview · Article · Apr 2011
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    ABSTRACT: Tonsilolith is unusual presentation of stone in tonsillar crypt. Etiology of tonsilolith is multifactorial and not usually diagnosed. Here a patient presented with bad smell from her breath and difficulty in swallowing. Radiological and biochemical investigation helped us to diagnose tonsilolith.
    No preview · Article · Jan 2011 · International Journal of Pharma and Bio Sciences
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    ABSTRACT: Stone(s) or calculi in the urinary tract are referred as urolithiasis. Clinically they usually presents with acute abdominal pain, urinary obstruction and hematuria. In present study we have analyzed 50 patients with uroliths for the chemical composition to know their cause, complications and measures for the preventing the occurrence of urolithiasis. We found that 90% of uroliths found to be calcium related stones and 10% were uric acid stones. Metabolic dysregulation, diet and environmental factors have a causative role in the etiogenesis of urolithiasis.
    No preview · Article · Jan 2011
  • B. D'Souza · Sreekantha · V. D'Souza
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    ABSTRACT: Background: Ovarian cancer is uncontrolled growth of abnormal ovarian cells. Ovarian cancer is the most common cause of cancer death among women, since no signs and symptoms are manifested at the early stage of the disease and the disease is diagnosed only in advanced stage. There is a strong need for a tumour marker for early diagnosis and to monitor the response of ovarian cancer patients to therapy Objective: To estimate serum amylase levels in benign, malignant ovarian tumour and ovarian cyst. Materials and Methods: Twenty five women with diagnosed ovarian cancer, twenty five benign ovarian tumor and twenty five ovarian cyst patients and 25 normal healthy women were selected for the study. Serum amylase activity is estimated by commercially available kit called CNPG 3 reagent set. Result: The serum amylase levels were significantly elevated (p< 0.001) in patients suffering from malignant ovarian tumours when compared to control. Conclusion: We conclude that estimation of serum amylase can be used as marker in the diagnosis and follow up cases of ovarian cancer patients.
    No preview · Article · Jan 2011 · International Journal of Pharma and Bio Sciences