[Show abstract][Hide abstract] ABSTRACT: Orotate phosphoribosyl transferase (OPRT) is an enzyme playing an important role in exertion of the effect of 5-fluorouracil (5-FU). A type of gene polymorphism, single nucleotide polymorphism (SNP), is considered to be a factor affecting individual differences in exertion of drug effects, and its analysis has recently made progress. We investigated the correlation between SNP of OPRT and 5-FU sensitivity in colon and rectal cancers. The subjects were 31 patients with colorectal cancer who underwent surgical excision between December 2003 and July 2004 at our department. Of SNP of OPRT, 638G/C, 1050T/A, and 1336A/G located in the coding region were analyzed by invader assay. The growth inhibition rate (% IR) of colorectal cancer by 5-FU was obtained by the CDDST method, and 5-FU sensitivity was compared among strains (wild-, homo-, and hetero-types) of each polymorphism. There was no relationship between the strains and 5-FU sensitivity in any of the SNPs. The investigated SNPs of OPRT may have no major influence on 5-FU sensitivity. However, there are many unknown factors in the relationship between SNP of OPRT and 5-FU sensitivity, and SNP analysis of other regions is necessary.
No preview · Article · Feb 2006 · Oncology Reports
[Show abstract][Hide abstract] ABSTRACT: Background: Respiratory suppression is observed during endoscopy under sedation. If respiratory suppression can be predicted before endoscopy, incidental complications can conceivably be prevented. In the present study, we focused on the relation between respiratory suppression from sedation and lung function.
Methods: A total of 211 patients underwent respiratory function tests before the surgical operation and gave written informed consent individually to participate in this study. We investigated the relation between respiratory suppression from sedation and lung function. During the endoscopic procedure, when blood oxygen saturation (SpO2) fell to below 90%, the patient was evaluated as ‘respiratory suppression present’.
Results: Sedation lowered SpO2 by an average of 6.0%, and was significantly lower than the prior to sedation blood oxygen saturation (PreSpO2). Compared to patients with SpO2 maintained up to 90%, patients with SpO2 fallen below 90% were significantly older, shorter in stature, lighter in bodyweight, and more commonly female. Furthermore, respiratory suppression from sedation was influenced by vital capacity (VC) and PreSpO2. Multivariate analysis was performed, and the receiver operating characteristic (ROC) curve constructed for the respiratory suppression prediction model based on age, height, VC and PreSpO2 yielded area under the curve (AUC) of 0.79. As VCpredict can be calculated from age and height, the three variables of age, height and VC in the above model were substituted with VCpredict resulting in a two-factor model based on VCpredict and PreSpO2. The ROC curve of the two-factor model had AUC of 0.77, which was slightly decreased but by no means inferior.
Conclusion: Predicting respiratory suppression from VCpredict and PreSpO2 is clinically relevant with the additional benefit of simplicity.
No preview · Article · Dec 2005 · Digestive Endoscopy