Wen-Hong Fan

National Institutes for Food and Drug Control, China, Peping, Beijing, China

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Publications (11)23.65 Total impact

  • No preview · Article · Jan 2013 · CNS Neuroscience & Therapeutics
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    ABSTRACT: In vivo electroporation works as an effective method to transfer exogenous genes into postnatal rodent forebrain. Nevertheless, two deficiencies were found in the reported methods. First, surgical operation brings unnecessary trauma to newborn pups. Second, the procedure was complicated and the transfection efficiency was relatively low. Here we improved the previous electroporation method and make it more simple and efficient. The pulse voltage was decreased to 90 v. DNA injection into one pup's forebrain could be completed within 30 s without any surgical operation. More than 94% of injected neonates survived. Almost 100% of the survivors expressed the introduced gene and the expression persists as long as 20 days after injection. Thus, this method offers a powerful new way for gene function study in postnatal neurogenesis and neural development.
    No preview · Article · Mar 2012 · Neurochemical Research
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    Cheng Tan · Wen-Hong Fan · Wen-Xiong Wang
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    ABSTRACT: Titanium dioxide nanoparticles (nano-TiO(2)) are now widely applied in consumer products, and the dispersion of nano-TiO(2) may adsorb metals and modify their behavior and bioavailability in the aquatic environment. In the present study, the aqueous uptake, dietary assimilation efficiency (AE), and efflux rate constant (k(e)) of two toxic metals (cadmium-Cd, and zinc-Zn) adsorbed on nano-TiO(2) in a freshwater zooplankton Daphnia magna were quantified. The biokinetics was then compared to daphnids that were exposed only to dissolved metals as controls. The aqueous uptake of Cd and Zn involved an initial rapid uptake and then an apparent saturation, and the uptake of metals was accompanied by an ingestion of nano-TiO(2). The AEs of Cd and Zn adsorbed on nano-TiO(2) were 24.6 ± 2.4-44.5 ± 3.7% and 30.4 ± 3.4-51.8 ± 5.0%, respectively, and decreased with increasing concentrations of nano-TiO(2). Furthermore, the difference between the AEs of Cd and Zn indicated that the desorption of Cd and Zn from nano-TiO(2) may have occurred within the gut of daphnids. With the use of algae as carrier, the AEs of Cd and Zn adsorbed on nano-TiO(2) were significantly higher than those of Cd and Zn directly from nano-TiO(2). The efflux rate constants of Cd and Zn adsorbed on nano-TiO(2) in the zooplankton were significantly lower than those of Cd and Zn not adsorbed on nano-TiO(2). Our study shows that the uptake and retention of toxic metals is enhanced when they are adsorbed on nano-TiO(2), and suggests more attention be paid to the potential influences of nano-TiO(2) on the bioavailability and toxicity of other contaminants.
    Preview · Article · Nov 2011 · Environmental Science & Technology
  • Chun-Mei Zhao · Wen-Hong Fan · Wen-Xiong Wang
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    ABSTRACT: Among the many toxic metals, the biokinetics of copper (Cu) in the freshwater cladoceran Daphnia magna have not been studied due to the lack of an ideal radiotracer. In the present study, a gamma radiotracer, ⁶⁷Cu (half-life = 61.9 h), was used to study the uptake of copper from the dissolved and dietary phase and efflux in D. magna, an important toxicity testing species. The influx rate of Cu from the dissolved phase increased with dissolved Cu concentration, with a calculated uptake rate constant of 0.055 L/g/h. The assimilation efficiency (AE) of Cu decreased significantly (from 92 to 16%) as the available food concentration increased, and the AE differed among the food types. As low as 1% of Cu AE was found in daphnids fed high concentrations (1.54 mg/L) of the green algae Chlorella pyrenoidosa. The AE decreased linearly as the ingestion rate of the daphnids increased. The efflux rate constant was 0.20/d at high food concentrations. Excretion accounted for 82 to 94% of total Cu loss from the animals, although Cu also was transferred maternally from female adults to their offspring. Under conditions of high food concentrations, approximately 6.5% of the mother's Cu was transferred to the offspring over 7 d. It was concluded that Cu accumulation is dominated by uptake from dietary sources, and there is a substantial need to understand the dietary toxicity of Cu to daphnids. The present study has implications for the choice of food particles in conducting the Cu toxicity testing in cladocerans.
    No preview · Article · Nov 2009 · Environmental Toxicology and Chemistry
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    ABSTRACT: Human DIXDC1 is a member of Dishevelled-Axin (DIX) domain containing gene family which plays important roles in Wnt signaling and neural development. In this report, we first confirmed that expression of Ccd1, a mouse homologous gene of DIXDC1, was up-regulated in embryonic developing nervous system. Further studies showed that Ccd1 was expressed specifically in neurons and colocalized with early neuronal marker Tuj1. During the aggregation induced by RA and neuronal differentiation of embryonic carcinoma P19 cells, expressions of Ccd1 as well as Wnt-1 and N-cadherin were dramatically increased. Stable overexpression of DIXDC1 in P19 cells promoted the neuronal differentiation. P19 cells overexpressing DIXDC1 but not the control P19 cells could differentiate into Tuj1 positive cells with RA induction for only 2 days. Meanwhile, we also found that overexpression of DIXDC1 facilitated the expression of Wnt1 and bHLHs during aggregation and differentiation, respectively, while inhibited gliogenesis by down-regulating the expression of GFAP in P19 cells. Thus, our finding suggested that DIXDC1 might play an important role during neurogenesis, overexpression of DIXDC1 in embryonic carcinoma P19 cells promoted neuronal differentiation, and inhibited gliogenesis induced by retinoic acid.
    Full-text · Article · Feb 2009 · Cellular and Molecular Neurobiology
  • Wen-Hong Fan · Ge Tang · Chun-Mei Zhao · Yong Duan · Rong Zhang
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    ABSTRACT: Metal accumulation (quantified as body burden) and the responses of two biomarkers (metallothionein [MT] induction and superoxide dismutase [SOD] activity) in a freshwater cladoceran Daphnia magna were quantified after being exposed to different concentrations of Cd and Zn for 3 d. The Cd and Zn body burdens increased with increasing exposure concentrations. The responses of biomarkers were metal-specific and concentration-dependent. As a detoxification mechanism, MT was induced by exposure to Cd or Zn or Cd + Zn and was dependent on the exposure concentrations. Low concentrations of Cd or Zn exposure increased the SOD activity, which was, however, inhibited at higher metal exposure concentrations. Metallothionein concentrations in daphnids were significantly related to the metal body burden. The SOD activity was inversely related to Cd body burden, while it was independent of the Zn body burden. Survivorship of D. magna was high at lower Cd/MT ratios in the animals but then decreased linearly when the ratio exceeded six. In contrast, the relationship between survivorship and Zn/MT ratio was different due to the essentiality of Zn. Overall, MT induction was a useful indicator of metal pollution in cladocerans.
    No preview · Article · Oct 2008 · Environmental Toxicology and Chemistry
  • Yan Wu · Xiao-Tang Jing · Xin Ma · Wen-Hong Fan · Ming Fan
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    ABSTRACT: By using of Escherichia coli DH5alpha to express GST-Ccd1 fusion protein and which was purified by affinity chromatographic separation. The purified target protein was used to immunize rabbits to prepare polyclonal antibody. The previously constructed recombinant prokaryotic expression vector pGEX-5X-1-Ccd1 was transformed into Escherichia coli DH5alpha and was induced to expression by IPTG. The recombinant target protein was expressed with soluble state in Escherichia coli expression system which was separated and purified by chromatographic column stuffed with glutathione Sepharose 4B. The prepared antigen was used to immunize rabbits to get anti-Ccd1 specific rabbit original polyclonal antibody. ELISA data demonstrated that the antibody titer of the serum was up to 1:40 000. Immunohistochemistry analysis indicated that the "home-made" antibody had a specific interaction with Ccd1 protein and which could be used for extended experimental research. The anti-Ccd1 polyclonal antibody we had prepared had a high quality of potency and specificity. The antibody was demonstrated by experiments that which could totally fulfill the requirement of immunoblotting and immunohistochemistry study of Ccd1. The antibody provided an useful experimental tool to profoundly research the tissue expression profile, intercellular location and biological function of Ccd1.
    No preview · Article · Feb 2008 · Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology
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    ABSTRACT: To identify the genes differentially expressed in development of human glioma, and to study the expression of some genes in different grade gliomas. Oligonucleotide microarray (including 218 genes related to neural system development) was adopted and hybridized with probes which were prepared from the total RNAs of glioma specimens and normal brain tissues. Differentially expressed genes between the normal tissues and glioma tissues were assayed after scanning oligonuceltide microarray with ScanArray 4000, and some of these genes such as smad1, Hmp19 and TRIP3 were verified by real-time quantitative PCR(real-time-Q-PCR) method. In comparison with the genes in the normal brain tissue, 5 down-regulated and 5 up-regulated genes in glioma specimens were revealed by means of microarrays, and the expression of smad1, Hmp19 and TRIP3 were verified by real-time-Q-PCR assay. Multiple genes play important roles in development of glioma. cDNA microarray technology is a powerful technique in screening for differentially expressed genes between glioma tissues and normal brain tissues. This study is helpful for judgement of invasion and prognosis of gliomas, and provides more target genes for targeted therapy.
    No preview · Article · May 2007 · Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics
  • Jun-Die Fan · Ling-Ling Zhu · Wen-Hong Fan

    No preview · Article · Feb 2007 · Sheng li ke xue jin zhan [Progress in physiology]
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    ABSTRACT: Intermittent hypoxia has been found to prevent brain injury and to have a protective role in the CNS. To address the possible causes of this phenomenon, we made investigative effort to find out whether intermittent hypoxia affects neurogenesis in the adult rat brain by examining the newly divided cells in the subventricular zone (SVZ) and dentate gyrus (DG). The adult rats were treated with 3000 and 5000 m high altitude 4 h per day for 2 weeks consecutively. 5-Bromo-2-deoxyuridine-5-monophosphate (BrdU) immunocytochemistry demonstrated that the BrdU-labeled cells in the SVZ and DG increased after 3000 and 5000 m intermittent hypoxia. The number of BrdU-labeled cells in the SVZ returned to normal level 4 weeks following intermittent hypoxia. However, the BrdU-labeled cells in the DG had a twofold increase 4 weeks subsequent to intermittent hypoxia. From these data, we conclude that intermittent hypoxia facilitates the proliferation of neural stem cells in situ, and that the newly divided cells in the SVZ and DG react differently to hypoxia. We are convinced by these findings that the proliferation of neural stem cells in SVZ and DG may contribute to adaptive changes following intermittent hypoxia.
    No preview · Article · Oct 2005 · Brain Research
  • Wen-Hong Fan · Min Li · Lu-Ming Wang · Ming Fan · Cheng Xing
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    ABSTRACT: To investigate the effect of hyperthermia on apoptosis of cultivated striatum neurons in the rat. After 30 min hyperthermia in 43 degrees, the Ca2+ concentration, the mitochondria membrane potential of the neuron were detected by Laser Scanning Confocal Microscopy (LSCM). The apoptosis of striatum neurons was detected by TUNEL staining. Heat stress at 43 degrees C for 40 min caused an increase in the Ca2+ concentration of striatum neurons and a decrease in the mitochondria membrane potential of the striatum neurons. The striatum shows more apoptosis neurons after heat stress.
    No preview · Article · Nov 2004 · Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology

Publication Stats

165 Citations
23.65 Total Impact Points


  • 2012-2013
    • National Institutes for Food and Drug Control, China
      Peping, Beijing, China
  • 2008-2011
    • Beijing University of Aeronautics and Astronautics (Beihang University)
      • Department of Environmental Science and Engineering
      Peping, Beijing, China
  • 2004-2008
    • Academy of Military Medical Sciences
      T’ien-ching-shih, Tianjin Shi, China