Publications (33)

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    Jean-Pierre Wolf · Malika Bouhaddi · Francis Louisy · [...] · Jacques Regnard
    [Show abstract] [Hide abstract] ABSTRACT: In the present study, the effects of L-dopa treatment on cardiovascular variables and peripheral venous tone were assessed in 13 patients with Parkinson's disease (PD) with Hoehn and Yahr stages 1-4. Patients were investigated once with their regular treatment and once after 12 h of interruption of L-dopa treatment. L-Dopa intake significantly reduced systolic and diastolic blood pressure, heart rate and plasma noradrenaline and adrenaline in both the supine and upright (60 degrees ) positions. A significant reduction in stroke volume and cardiac output was also seen with L-dopa. The vascular status of the legs was assessed through thigh compression during leg weighing, a new technique developed in our laboratory. Healthy subjects were used to demonstrate that this technique provided reproducible results, consistent with those provided by strain gauge plethysmography of the calf. When using this technique in patients with PD, L-dopa caused a significant lowering of vascular tone in the lower limbs as shown, in particular, by an increase in venous distensibility. Combined with the results of the orthostatic tilting, these findings support that the treatment-linked lowering of plasma noradrenaline in patients with PD was concomitant with a significant reduction in blood pressure, heart rate and vascular tone in the lower limbs. These pharmacological side-effects contributed to reduce venous return and arterial blood pressure which, together with a lowered heart rate, worsened the haemodynamic status.
    Full-text Article · Apr 2006 · Clinical Science
  • Jean Pierre Wolf · Malika Bouhaddi · F. Louisy · [...] · Jacques Regnard
    Article · Jan 2006
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    [Show abstract] [Hide abstract] ABSTRACT: The objectives to determine both the contribution to orthostatic intolerance (OI) of calf venous volume during a stand-test, and the effects of a combined eccentric-concentric resistance exercise countermeasure on both vein response to orthostatic test and OI, after 90-day head-down tilt bed-rest (HDT). The subjects consisted of a control group (Co-gr, n = 9) and an exercise countermeasure group (CM-gr, n = 9). Calf volume and vein cross-sectional area (CSA) were assessed by plethysmography and echography during pre- and post-HDT stand-tests. From supine to standing (post-HDT), the tibial and gastrocnemius vein CSA increased significantly in intolerant subjects (tibial vein, +122% from pre-HDT; gastrocnemius veins, +145%; P < 0.05) whereas it did not in tolerant subjects. Intolerant subjects tended to have a higher increase in calf filling volume than tolerant subjects, in both sitting and standing positions. The countermeasure did not reduce OI. Absolute calf volume decreased similarly in both groups. Tibial and gastrocnemius vein CSA at rest did not change during HDT in either group. During the post-HDT stand-test, the calf filling volume increased more in the CM-gr than in the Co-gr both in the sitting (+1.3 +/- 5.1%, vs. -7.3 +/- 4.3%; P < 0.05) and the standing positions (+56.1 +/- 23.7% vs. +1.6 +/- 9.6%; P < 0.05). The volume ejected by the muscle venous pump increased only in the CM-gr (+38.3 +/- 21.8%). This study showed that intolerant subjects had a higher increase in vein CSA in the standing position and a tendency to present a higher calf filling volume in the sitting and standing positions. It also showed that a combined eccentric-concentric resistance exercise countermeasure had no effects on either post-HDT OI or on the venous parameters related to it.
    Full-text Article · Dec 2004 · The Journal of Physiology
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    [Show abstract] [Hide abstract] ABSTRACT: This study was undertaken to determine whether or not elastic compression stockings (ECS) can be used in elderly sportsmen to increase performance and leg pain recovery between two maximal exercises. For 2 weeks, 12 trained elderly cyclists, 63 (3) years old, performed two 5-min maximal exercises, Plim1 and Plim2, separated by an 80-min recovery period, twice a week with a 2-day rest interval. During the 80-min recovery period, they randomly wore or did not wear grip-top ECS Ganzoni-Sigvaris. ECS exerted a 44 hPa pressure at the ankle. Blood lactate concentrations, hematocrit, and plasma volume were measured after a 60-min rest and every 20 min during recovery. Leg sensations were assessed with a questionnaire. The decrease in maximal power between Plim1 and Plim2 was lower when wearing the ECS during the 80-min recovery period; when expressed as a percentage of Plim1, the difference reached 2.1 (1.4)%, P < 0.01. Between the two exercises, blood lactate concentrations and hematocrit were significantly decreased when wearing ECS. The increase in plasma volume was not significant. The 12 cyclists stated that wearing the ECS had a positive effect on their leg pain. Ten of the cyclists thought that it could have influenced their performance. However, no relationship was found between the gain in performance and the leg pain sensation. It was concluded that wearing ECS during an 80-min recovery period significantly increased subsequent performance. This was associated with a reduction in lactate and hematocrit.
    Full-text Article · Dec 2004 · Arbeitsphysiologie
  • [Show abstract] [Hide abstract] ABSTRACT: The aim of the present study was to evaluate the fluid shift in a simulated microgravity experiment and to test the use of thigh cuffs to help alleviate the problem. The change in skin thickness was assessed by a 20 MHz B-scan ultrasound device. This was performed on eight volunteers who underwent two successive 7-day periods of -6 degrees anti-orthostatic bed-rest, with or without the daytime use of thigh cuffs. The thigh cuffs were used to counteract the development of facial oedema. In the control group (without thigh cuffs), the results showed a steady increase in skin thickness of the combined dermis and hypodermis of the forehead and a reduction of the thickness of this tissue on the tibia. For the countermeasure group, although thigh cuffs were only employed during the daytime - being removed at night - their use reduced the amplitude and kinetics of the fluid shift, resulting in greater beneficial effects at the end of the day than early in the morning. These results of objective measurements of skin made using a non-invasive high frequency ultrasonography method confirm reports by cosmonauts of a reduction in facial oedema and a more 'comfortable' adaptation to microgravity by the use of thigh cuffs during space flight. This system is potentially promising for investigating fluid shifts in the skin and may prove useful in the evaluation of some oedematous skin diseases, as well as their therapy.
    Article · Aug 2001 · European Journal of Ultrasound
  • Francis Louisy · Didier Cauquil · Claudie Andre-Deshays · [...] · Gallina Fomina
    [Show abstract] [Hide abstract] ABSTRACT: A new approach to the study of peripheral vascular hemodynamics in microgravity was initiated by designing an alternative methodology: air plethysmography (AP). This is the only technique that enables the quantification of vascular hemodynamics from gross measurements performed on the limbs. This paper reports a description of the device and of the measurement protocol. A comparative study showed that AP yields results that are well correlated, although not in agreement, with those obtained by means of the reference method in the laboratory (mercury strain gauge plethysmography, MSGP; for venous capacity, correlation coefficient r = 0.8, P < 0.0001, limits of agreement--0.9 ml.100 ml-1 and 1.4 ml.100 ml-1; for arterial flow index, correlation coefficient r = 0.7, P < 0.0006, limits of agreements -20.4 ml.min-1 and 37.2 ml.min-1; for half-emptying time, correlation coefficient r = 0.9, P < 0.0001, limits of agreement -0.88 s and 0.77 s), and that are characterized by good reproducibility (coefficient of variation in general lower than 12%). Preliminary findings during spaceflight, on board the Mir Space Station, yielded data that is expected to improve our knowledge of vascular deconditioning in conditions of weightlessness.
    Article · Aug 2001 · Arbeitsphysiologie
  • J.-T. Aubert · F. Louisy · P. Arbeille · C. Ribreau
    Article · Jan 2001
  • A. Pavy-Le Traon · F Louisy · P Vasseur-Clausen · [...] · C Gharib
    [Show abstract] [Hide abstract] ABSTRACT: Various factors may contribute to orthostatic intolerance (OI) observed after space flights or simulated weightlessness such as bed rest experiments: individual physical and physiological factors (arterial blood pressure (BP), height), physiological changes induced by real or simulated weightlessness (hypovolaemia, increase in venous distensibility), and space flight or simulation conditions (duration and counter-measure application). Our purpose was to test which of these factors were dominant in contributing to the OI. This was assessed in 47 healthy men participating in bed rest experiments of 4, 14, 28, 30 and 42 days, with or without counter-measures (medical stockings, lower-body negative pressure (LBNP), LBNP + muscular exercise). Nineteen subjects did not finish the orthostatic test (60 degrees head-up tilt or stand test) after bed rest. The occurrence of OI was associated with greater height, low resting BP, greater changes in resting lower-limb venous distensibility throughout the bed rest, and absence of counter-measures.
    Article · Oct 1999 · Clinical Physiology
  • F Louisy · P Schroiff · A Güell
    [Show abstract] [Hide abstract] ABSTRACT: Leg venous hemodynamics [venous distensibility index (VDI), arterial flow index (AFI), half-emptying time (T1/2)], and leg volumes (LV) were assessed by mercury strain-gauge plethysmography with venous occlusion and volometry, respectively, in seven men before, during, and after 42 days of 6 degrees head-down bed rest. Results showed a high increase in VDI up to day 26 of bed rest (+50% vs. control at day 26, P < 0.05), which tended to subside thereafter (+20% increase vs. control value at day 41, P < 0.05). VDI changes were associated with parallel changes in T1/2 (+54% vs. control at day 26 of bed rest, P < 0.05, and +25% vs. control at day 41, P < 0.05) and with a decrease in AFI (-49% at day 41 vs. P < 0.05). LV continuously decreased throughout bed rest (-13% vs. control at day 41, P < 0.05) but was correlated with VDI only during the first month of bed rest. These results show that during long-term 6 degrees head-down bed rest alterations of leg venous compliance are associated with impairment of venous emptying capacities and arterial flow. Changes in skeletal muscle mass and fluid shifts may account for venous changes during the first month of bed rest but, subsequently, other physiological factors, to be determined, may also be involved in leg venous hemodynamic alterations.
    Article · Jun 1997 · Journal of Applied Physiology
  • F Louisy · J C Jouanin · CY Guezennec
    [Show abstract] [Hide abstract] ABSTRACT: Leg volume changes were assessed in healthy volunteers by mercury strain gauge postural plethysmography in order to study the effects of different types of physical training on lower limb venous distensibility and emptying. Seven endurance trained subjects (E), seven muscle strength trained subjects (M) and seven sedentary subjects (S) were submitted to a tilt table test. The test measured leg filling volumes FV (ml.100 ml-1) during 30 head-up tilt, half-emptying time T1/2 (seconds) and venous output at the 6th second of emptying (VO6) (ml.100 ml-1.min-1) during return to horizontal position. Comparative tests were made using an ANOVA test. Results showed significantly higher values for FV and VO6 in E (FV: 3.9 +/- 0.2 ml.100 ml-1 in E vs 2.5 +/- 0.2 and 2.2 +/- 0.2 ml.100 ml-1 in M and S, respectively; VO6: 9.2 +/- 1.1 ml.100 ml-1.min-1 in E vs 6.9 +/- 0.7 and 5.5 +/- 0.5 ml.100 ml-1.min-1 in M and S, respectively) whereas no significant differences were observed between groups for T1/2. M and S had the same physiological responses to orthostatic stress. Endurance training seems to increase lower limb venous distensibility without affecting venous emptying and venous return from the lower limbs. These results suggest that the greater venous distensibility in endurance-trained subjects does not result from alterations of the visco-elastic properties of deep vein walls and that it is probably a physical response to hypervolemia consecutive to chronic aerobic training.
    Article · Feb 1997 · International Journal of Sports Medicine
  • F Louisy · C Andre-Deshays · P Schroiff · [...] · G Fomina
    [Show abstract] [Hide abstract] ABSTRACT: First results on changes in vascular physiology in the first french spationaut during the French-russian spatial mission CASSIOPEE are reported in this paper. The data, obtained by Air Plethysmography during flight, evidence at the level of lower limbs alterations of venous filling and emptying characteristics (the latter particularly depending on the muscular pump function of the calf) and of capillary circulation.
    Article · Feb 1997 · Travaux scientifiques des chercheurs du Service de santé des armées durant l'année ... / République Française, Ministère de la défense, Direction centrale du Service de santé des armées
  • [Show abstract] [Hide abstract] ABSTRACT: Leg volume changes were assessed in healthy volunteers by mercury strain gauge postural plethysmography in order to study the effects of different types of physical training on lower limb venous distensibility and emptying. Seven endurance trained subjects (E), seven muscle strength trained subjects (M) and seven sedentary subjects (S) were submitted to a tilt table test. The test measured leg filling volumes FV (ml·100 ml-1) during 30* head-up tilt, half-emptying time T1/2 (seconds) and venous output at the 6th second of emptying (VO6) (ml ·100 ml-1 · min-1) during return to horizontal position. Comparative tests were made using an AIMOVA test. Results showed significantly higher values for FV and VO6 in E (FV: 3.9 ±0.2 ml · 100 ml-1 in E vs 2.5 ± 0.2 and 2.2 ±0.2 ml-100 ml-1 in M and S, respectively; VO6:9.2 ± 1.1 ml ·100 ml-1 · min-1 in E vs 6.9 ± 0.7 and 5.5 ± 0.5 ml · 100 ml-1 · min-1 in M and S. respectively) whereas no significant differences were observed between groups for T1/2. M and S had the same physiological responses to orthostatic stress. Endurance training seems to increase lower limb venous distensibility without affecting venous emptying and venous return from the lower limbs. These results suggest that the greater venous distensibility in endurance-trained subjects does not result from alterations of the visco-elastic properties of deep vein walls and that it is probably a physical response to hypervolemia consecutive to chronic aerobic training.
    Article · Jan 1997
  • F Louisy · P Schroiff
    [Show abstract] [Hide abstract] ABSTRACT: The study and follow-up of certain physiological adaptations in microgravity, particularly vascular and venous ones, require the use of reliable equipment that yields results well correlated with data provided by equipment usually used in clinical examinations. The purpose of the present study was to evaluate the reproducibility of results obtained using a new type of plethysmography, plethysmography with optoelectronic sensors or volometry, and to verify whether these results correlate with those yielded by mercury strain gauge plethysmography. The plethysmograph is a device which permits measuring limb volume by means of an infra-red light transmitter-receiver system that calculates cross-sectional areas at 220 successive points of a given limb segment. Calf venous capacity (calf volume measured after 50 mm Hg venous occlusion in the thigh) was measured on 27 subjects using volometry and mercury strain gauge plethysmography used as reference method. Results showed a good correlation (r = 0.8, p < 0.001) and a statistically identical reproducibility between the two methods even though venous capacity measurements obtained using these two techniques were not superimposable because they did not use the same model for limb volume measurements. Volometry therefore appears to be a technique of interest for the future, to monitor vascular and muscular physiological parameters in astronauts during long-term microgravity exposure.
    Article · Jan 1996 · Aviation Space and Environmental Medicine
  • F Louisy · P Schroiff · C Y Guezennec · A Guell
    [Show abstract] [Hide abstract] ABSTRACT: Seven healthy subjects were submitted to a 42-day head down bedrest, where leg venous compliance (venous distensibility index VDI) and leg volumes were assessed by mercury strain gauge plethysmography with venous occlusion and optoelectronic plethysmography, respectively. Plethysmographic and volometric measurements were made, before, during (at days 1, 4, 7, 14, 21, 26, 34 and 41), and after bedrest (days 1, 4, 7, 11 and 30 of the recovery period). Results showed a continuous decrease in leg volumes throughout bedrest, when VDI increased until day 26 of bedrest, and then decreased afterwards. The recovery period was characterized by a rapid return of VDI to prebedrest levels while leg volumes progressively normalised. These results showed that leg venous compliance changes are not always dependent upon skeletal muscle changes, and that factors other than size of muscle compartment are able to determine increases in leg venous compliance during long-term bedrest.
    Article · Oct 1995 · Acta Astronautica
  • F. Louisy · P. Schroiff · CY Guezennec · A. Güell
    [Show abstract] [Hide abstract] ABSTRACT: Seven healthy subjects were submitted to a 42-day head down bedrest, where leg venous compliance (venous distensibity index VDI) and leg volumes were assessed by mercury strain gauge plethysmography with venous occlusion and optoelectronic plethysmography, respectively. Plethysmographic and volometric measurements were made, before, during (at days 1, 4, 7, 14, 21, 26, 34 and 41), and after bedrest (days 1, 4, 7, 11 and 30 of the recovery period). Results showed a continuous decrease in leg volumes throughout bedrest, when VDI increased until day 26 of bedrest, and then decreased afterwards. The recovery period was characterized by a rapid return of VDI to prebedrest levels while leg volumes progressively normalised. These results showed that leg venous compliance changes are not always dependant upon skeletal muscle changes, and that factors other than size of muscle compartment are able to determine increases in leg venous compliance during long-term bedrest.
    Article · Oct 1995 · Acta Astronautica
  • F Louisy · P Berry · J F Marini · [...] · C Y Guezennec
    [Show abstract] [Hide abstract] ABSTRACT: In order to test the hypothesis that increases in calf venous distensibility in microgravity are partly due to the changes affecting the surrounding skeletal muscles (muscular atrophy), 12 healthy volunteers were exposed for 28 d to microgravity simulated by -6 degrees head-down bed rest. Half these subjects were exposed to countermeasures during bed rest: a) repeated LBNP (Lower Body Negative Pressure) sequences starting on the 15th d with one 15 min sequence at -35 mb, every other day from the 15th until the 21st d, and then every day until the end of bed rest; b) physical training including isotonic type exercise and isometric or isokinetic work by all muscle mass of upper and lower limbs (from the 8th until the 28th d). The other six subjects forming the control group were not subjected to any countermeasure. Calf venous hemodynamics were determined by mercury strain gauge plethysmography with venous occlusion. Distensibility (delta Vmax) and venous emptying (venous outflow at the 6th s of emptying: VO6, half-emptying time: T1/2, maximum venous outflow (MVO) could also be measured. Nuclear magnetic resonance (NMR) was used to study changes in volume of calf muscles. Plethysmographic measurements made for each subject prior to, during (once a week), and after bed rest show a parallel increase in calf venous distensibility in both groups of subjects until the 20th d of bed rest. Filling and emptying times then tended to stabilize in the group treated with countermeasures (group CM) whereas high venous distensibility was observed until the end of bed rest and 5 d thereafter in the control group (group C).(ABSTRACT TRUNCATED AT 250 WORDS)
    Article · Jul 1995 · Aviation Space and Environmental Medicine
  • F Louisy · P Schroiff · C Y Guezennec · A Güell
    [Show abstract] [Hide abstract] ABSTRACT: Exposure to actual or simulated microgravity is known to result in changes in lower limb venous compliance or distensibility which may play a role in post-bedrest or postflight orthostatic intolerance. Venous deconditioning has only been described in terms of changes in vascular compliance or distensibility. But a complete understanding of changes in venous hemodynamics and cardiovascular regulation occurring under these conditions has to take into account changes in emptying capacities of the veins which influence venous return, cardiac filling, and cardiac output regulation. Moreover, few data are available about the course of changes in venous hemodynamics for periods of simulated microgravity longer than 4 weeks. The purpose of this investigation was to measure parameters of venous compliance and venous emptying before, during, and after a 42-day period of bedrest at -6 degrees head-down tilt for a better understanding of long term venous physiological adaptation to microgravity.
    Article · Feb 1995 · Journal of gravitational physiology: a journal of the International Society for Gravitational Physiology
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    F. Louisy · P. Berry · J.F. Marini · [...] · C.Y. Guezennec
    Full-text Article · Jul 1994
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    F. Louisy · L. Pupier · C.Y. Guezennec · A. Güell
    Full-text Article · Jul 1994
  • F Louisy · CY Guezennec · A Güell
    [Show abstract] [Hide abstract] ABSTRACT: When contemplating future trips to the Moon whose gravity is one sixth of Earth gravity, the question is to know what the adaptive changes in the lower limb venous system would be. In fact, one can suppose that the presence of a partial gravity on the Moon would be able to attenuate venous hemodynamics adaptative changes observed in microgravity. In the present experiment changes in the venous hemodynamics of lower limbs have been studied with mercury strain gauge plethysmography during a simulated Moon mission including a 4 day trip to the Moon (-6 degrees bedrest), a stay of 6 days on the Moon (+11 degrees bedrest), and a 4-day trip back to Earth (-6 degrees bedrest). It was previously demonstrated that +11 degrees bedrest was a good model to simulate the effects of lunar gravity on the cardiovascular system (Vernikos-Danellis J 1986, personnal communication).
    Article · Jun 1994 · Journal of gravitational physiology: a journal of the International Society for Gravitational Physiology