Jack E. Staub

Utah State University, لوگان، اوهایو, Ohio, United States

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Publications (175)270.33 Total impact

  • Matthew D. Robbins · Jack E. Staub · B. Shaun Bushman

    No preview · Article · Jan 2016 · Euphytica
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    ABSTRACT: Most melon (Cucumis melo L.) breeding lines in South Korea display andromonoecious sex expression, which necessitates laborious hand emasculation during F1 hybrid seed production. Thus, there is a need to develop monoecious sex types in elite germplasm to obviate self-pollination. Sex expression is associated with floral ethylene production, which, in monecious melon plants, is associated with the A locus. Our study was conducted to develop molecular markers for selection of monoecious plants based on sequence variation inherent in the CmACS-7 gene [encoding 1-aminocyclopropane-1-carboxylic acid synthase (ACS)] that is associated with ethylene production. Full-length CmACS-7 sequences were cloned from a monoecious (MO23) and an andromonoecious (AM24) line. The alignment of those CmACS-7 sequences revealed a single nucleotide polymorphism (SNP; C170T) in exon 1 and an 18 bp indel in the 3′-untranslated region (UTR) of between MO23 and AM24, which was then used to develop a cleaved amplified polymorphic sequence (CAPS) (EX1-C170T) and a sequence characterized amplified region (SCAR) marker (T1ex), respectively. The sex expression and the T1ex SCAR-based genotype of 442 F2 plants derived from a MO23 × AM24 cross was determined. Monoecy and andromonoecy segregated in a 3:1 ratio in F2 progeny, where the sex type of 429 plants (13 plants not classified) co-segregated with the SCAR marker, demonstrating that sex expression regulated by CmACS-7 is controlled by a single dominant gene and that it confers monoecy in line MO23. Allelic variation in 112 geographically diverse melon lines for CmACS-7 as accessed by CAPS EX1-C170T and SCAR T1ex markers indicated that the: 1) exon 1 of CmACS-7 is highly conserved and the SNP/sex expression association detected is highly predictable making it potentially useful for marker-based selection of monoecious plants, and; 2) 18 bp indel mutation in the 3′-UTR was present in various lengths depending on different monoecious melon germplasm. © 2015, Korean Society for Horticultural Science and Springer-Verlag GmbH.
    No preview · Article · Aug 2015 · Horticulture, Environment and Biotechnology

  • No preview · Article · Aug 2015 · HortScience: a publication of the American Society for Horticultural Science
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    ABSTRACT: Background and aims Native grasses planted or growing on sites contaminated by heavy metals should be safe for livestock and wildlife. Plant breeders seek to identify genes and quantitative trait loci (QTLs) controlling trace element variation among these grasses. Methods QTLs controlling forage mineral concentrations were mapped in a population derived from two perennial wildrye species, Leymus cinereus and Leymus triticoides, grown in soil contaminated with arsenic, cadmium, copper, lead, zinc, and other trace elements. These QTLs were aligned to the genome sequence of barley (Hordeum vulgare) for comparison to genes or QTLs controlling trace element uptake in other species and soils, including perennial wildrye grown in fertile soil. Results A total of 25 QTLs for 14 elements were detected on contaminated soil. Three of four zinc QTLs were conserved between fertile and contaminated soils, but no other QTLs were conserved across these test soils. Two homoeologous molybdenum QTLs were closely associated with MOT1 orthogenes, which encode one of two known molybdate transporters in plants, and possible candidate gene associations were identified for other heavy metal QTLs. Conclusions Results elucidate conserved and unparalleled mechanisms controlling trace element variation among different plants and soils, showing opportunities and challenges in plant breeding.
    Preview · Article · Jul 2015 · Plant and Soil
  • Asjad Ali · Sun Woong Bang · Sang-Min Chung · Jack E. Staub
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    ABSTRACT: Genetic transformation using foreign genes and the subsequent development of transgenic plants has been employed to develop enhanced elite germplasm. Although some skepticism exits regarding pollen tube-mediated gene transfer (PTT), reports demonstrating improved transformation efficiency with PTT systems are increasing and encouraging and the adoption of increasingly refined pollen-mediated methodologies may lead to species-dependent improvements in breeding. Here, we highlight PTT technology as an alternative to genetic transformation.
    No preview · Article · Dec 2014 · Plant Molecular Biology Reporter
  • Jack E. Staub · M.D. Robbins · Yingmei Ma · Paul G. Johnson
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    ABSTRACT: Continued reduction in limited natural resources worldwide increasingly necessitates the incorporation of low-maintenance and low-input plant materials into urban landscapes. Some fine-leaved Festuca grass species have been used in formal gardens and native urban landscapes because of their inherent tolerance to abiotic stresses, but native, ornamental types (tall and non-spreading with multicolored culms and panicles) are not common in landscapes of the western United States. A native fine-leaved Festuca collection made in Montana (designated FEID 9025897) by the U.S. Natural Resources Conservation Services possesses such ornamental characteristics but has not been evaluated for its horticultural potential. Therefore, a study was designed to assess its phenotypic and genotypic attributes by cloning 270 FEID 9025897 plants and evaluating them along with native F. idahoensis and F. ovina PIs (five) and commercial checks (five) for genetic diversity and plant morphology for 2 years (2010-11). Plant genetic constitution was determined using amplified fragment length polymorphism (AFLP) analysis. Plant height, width, biomass, relative vigor (visual rating of 0 = dead to 5 = green, abundant growth), persistence (number of plants alive per plot), and regrowth after clipping (visual rating of 0 = none to 5 = most) were estimated by evaluation of plants under replication at Hyde Park, UT. Based on AFLP-based coancestry analysis, FEID 9025897 plants possessed considerable genetic affinities with F. idahoensis. Morphological traits as averaged over both years varied in height (13.9 to 105.0 cm), width (9.9 to 66.2 cm), biomass (0 to 170.4 g), vigor (0.2 to 4.7), persistence (0 to 3.9), and regrowth (0 to 4.0). Based on these differences, 19 (7%) FEID 9025897 plants were identified for their ornamental potential that possessed multicolored (red, orange, and yellow) culms and varied in morphology with 2-year means of height (79.8 cm), width (45.2 cm), biomass (88.5 g), vigor (2.9), persistence (1.8), and regrowth (3.7). © 2014 by the American Society for Horticultural Science. All rights reserved.
    No preview · Article · Nov 2014 · Journal of the American Society for Horticultural Science. American Society for Horticultural Science
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    ABSTRACT: Compact and dwarfing vining habits in melon (Cucumis melo L.; 2n = 2x = 24) may have commercial importance since they can contribute to the promotion of concentrated fruit set and can be planted in higher plant densities than standard vining types. A study was designed to determine the genetics of dwarfism associated with a diminutive (short internodes) melon mutant line PNU-D1 (C. melo ssp. cantalupensis). PNU-D1 was crossed with inbred wild-type melon line PNU-WT1 (C. melo ssp. agrestis), and resultant F1 progeny were then self-pollinated to produce an F2 population that segregated as dwarf and vining plant types. Primary stem length of F2 progeny assessed under greenhouse conditions indicated that a single recessive gene, designated mdw1, controlled dwarfism in this population. To identify the chromosomal location associated with mdw1, an simple sequence repeat (SSR)-based genetic linkage map was constructed using 94 F2 progeny. Using 76 SSR markers positioned on 15 linkage groups spanning 462.84 cM, the location of mdw1 was localized to Chromosome 7. Using the putative dwarfing-associated genes, fine genetic mapping of the mdw1 genomic region was facilitated with 1,194 F2 progeny that defined the genetic distance between mdw1 and cytokinin oxidase gene, a candidate gene for compact growth habit (cp) in cucumber, to be 1.7 cM. The candidate gene ERECTA (serin/threonine kinase) and UBI (ubiquitin) were also mapped to genomic regions flanking mdw1 at distances of 0.6 and 1.2 cM, respectively.
    Full-text · Conference Paper · Oct 2014
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    ABSTRACT: The responses to chilling temperature of 12 Korean cucumber varieties was compared to those of two U.S.A. (previously determined cold tolerant NC76 and 'Chipper'), and Chinese and Japanese germplasms. Seedlings of each entry were exposed to 4 degrees C (Experiment 1) and 1 degrees C (Experiments 2 and 3) at the first-true leaf stage for eight and nine hours, respectively, under 80% relative humidity (RH) and 149 mu moles.m(-2).s(-1) photosynthetic photon flux (PPF). The chilling response [damage rating (DR)] of each accession was based on visual ratings (1 to 5) after treatment, where 1 = no damage, 2 = slight, 3 = moderate, 4 = advanced, and 5 = severe damage. Predictably the cumulative average DR of chilling tolerant line NC76 and 'Chipper' after chilling was 1 and 1.1, respectively. Korean 'Nacdongchungjang' was most sensitive to chilling temperatures [DR = 2.3] when compared to the other entries examined. The sensitivity to chilling of 'Nacdongchungjang' was followed by Chinese 'Dongguan' [DR = 1.7]. In contrast, 'Saeronchungjang' (DR = 1) and 'Janghyungnachap' (DR = 1) were the most chilling tolerant of the Korean accessions examined and equivalent to the response of line NC76 and 'Chipper'. Nevertheless, chloroplast type genotyping of these accessions with known chilling-linked sdCAPS genomic markers revealed genotypic differences between chilling tolerant lines (NC76 and 'Chipper') and all Korean lines examined.
    Full-text · Article · Apr 2014 · Wonye kwahak kisulchi = Korean journal of horticultural science and technology /
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    ABSTRACT: Chilling temperatures (<10 °C) may cause damages in cucumber plants (Cucumis sativus L.) during winter and early spring seasons. Inheritance of chilling injury in U.S. processing cucumber is controlled by cytoplasmic (maternally) and nuclear factors. To understand inheritance of chilling injury in Korean market-type cucumber, reciprocal crosses between chilling tolerant (CT1) and susceptible (CT4) lines produced F1 (CT1 × CT4) and F1 (CT4 × CT1) progenies. Reciprocal F2 (CT1 × CT4) and F2 (CT4 × CT1) populations were subsequently derived. Seedlings in the first true leaf stage were subjected to 4 °C for 8 h (08:00 to 16:00) and damage level was assessed visually using 1 (no damage) to 5 (severe damage) rating scale. Means of damage rating for reciprocal F1 (CT1 × CT4) and F1 (CT4 × CT1) progenies were 1.1 and 1.1, respectively. This indicates that tolerance for chilling stress at 4 °C in this germplasm is dominant. However, means of damage for F2 (CT1 × CT4) progenies and F2 (CT4 × CT1) progenies were 3.2 and 1.2, respectively. These data indicate that genetic control of chilling injury in these progenies is paternal. Based on the data, we hypothesize that line CT1 possesses a dominant nuclear factor that conditions chilling tolerance in both reciprocal F1s and a paternal factor(s) that lead chilling tolerance only in F2 (CT4 × CT1). These putative nuclear and paternal genetic factors are designated as Ch-1 and Ch-p, respectively.
    No preview · Article · Mar 2014 · Scientia Horticulturae
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    ABSTRACT: Rhizomes are prostrate subterranean stems that provide primitive mechanisms of vegetative dispersal, survival, and regrowth of perennial grasses and other monocots. The extent of rhizome proliferation varies greatly among grasses, being absent in cereals and other annuals, strictly confined in caespitose perennials, or highly invasive in some perennial weeds. However, genetic studies of rhizome proliferation are limited and genes controlling rhizomatous growth habit have not been elucidated. Quantitative trait loci (QTLs) controlling rhizome spreading were compared in reciprocal backcross populations derived from hybrids of rhizomatous creeping wildrye (Leymus triticoides) and caespitose basin wildrye (L. cinereus), which are perennial relatives of wheat. Two recessive QTLs were unique to the creeping wildrye backcross, one dominant QTL was unique to the basin wildrye backcross, and one additive QTL was detectable in reciprocal backcrosses with high log odds (LOD = 31.6) in the basin wildrye background. The dominant QTL located on linkage group (LG)-2a was aligned to a dominant rhizome orthogene (Rhz3) of perennial rice (Oryza longistamina) and perennial sorghum (Sorghum propinquum). Nonparametric 99 % confidence bounds of the 31.6-LOD QTL were localized to a distal 3.8-centiMorgan region of LG-6a, which corresponds to a 0.7-Mb region of Brachypodium Chromosome 3 containing 106 genes. An Aux/IAA auxin signal factor gene was located at the 31.6-LOD peak, which could explain the gravitropic and aphototropic behavior of rhizomes. Findings elucidate genetic mechanisms controlling rhizome development and architectural growth habit differences among plant species. Results have possible applications to improve perennial forage and turf grasses, extend the vegetative life cycle of annual cereals, such as wheat, or control the invasiveness of highly rhizomatous weeds such as quackgrass (Elymus repens).
    No preview · Article · Feb 2014 · MGG Molecular & General Genetics
  • Vanessa S. Gordon · Jack E. Staub
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    ABSTRACT: Environmental stresses such as chilling temperatures can decrease germination, emergence, flower and fruit development, marketable yield, and postharvest fruit storage longevity in cucumber (Cucumis sativus L.). While response to chilling injury in cucumber is controlled by simple plastidic (maternal) and nuclear (paternal) factors, no chilling tolerant U.S. processing varieties are commercially available. Furthermore, even though three single nucleotide polymorphic sites have been identified as plastid components associated with chilling tolerance in cucumber, it is not known how these factors interact with nuclear factors controlling economically important traits. Therefore, an experiment was designed to evaluate the rate of recovery of the chilling susceptible (cytoplasm) genotype during introgression backcrossing (IB), where it was used as a recurrent parent after the initial mating to a line possessing chilling tolerant cytoplasm (donor parent). Phenotypic yield and quality trait data were collected on processing type backcross progeny (BC1–5 and BC2S3) derived from an initial ‘Chipper’ (tolerant) × line M 29 (susceptible) mating, and rate of progression to the recurrent parent was determined by simple sequence repeat marker and morphological trait analyses. Substantial degrees of the recurrent parent phenotype and nuclear genome were recovered by the BC2 generation (P = 0.001), with nearly complete recovery of recurrent parental traits and its nuclear genome occurring by the BC3. General combining ability (GCA) of derived BC2S3 lines was significant for yield, yield/plant, length (L), diameter (D), and L:D ratios. The BC2S3 line GCA and rate of progression towards the recurrent parent for economically important traits suggests that elite chilling tolerant cucumber germplasm can be developed rapidly through IB and marker genotyping.
    No preview · Article · Jan 2014 · Euphytica
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    ABSTRACT: Fine-leaved Festuca valesiaca Schleich. ex Gaudin (2n = 2x–4x) is native to heavily-grazed, cold, semi-arid, Asian rangelands. However, its potential for low-maintenance turf applications in the semi-arid western United States and its relatedness to other agriculturally important Festuca species have not been investigated. Therefore, a project was designed to identify F. valesiaca accessions that possess horticultural potential when grown under semi-arid growing conditions and to characterize their relatedness to other Festuca species. In 2008, 12 F. valesiaca accessions originating from Kyrgyzstan and eight US. Festuca and one Lolium cultivar were transplanted as replicated, spaced plants to a field nursery at Blue Creek, Utah. Relative vigor, height, width, total biomass (aboveground dry matter yield), seed weight, and seed number were evaluated between 2009 and 2011. Plant height, width, and total biomass of the F. valesiaca accessions examined were approximately equal to the commercial control, ‘Cascade’ (F. rubra L. subsp. commutata Gaudin; 6x; chewings fescue). Plant vigor and seed weight of F. valesiaca accessions PI 659923, PI 659932, W6 30575, and W6 30588 under semi-arid conditions (~300 mm annual precipitation) were significantly (P F. valesiaca accessions were distinct from a majority of the other Festuca accessions examined. These F. valesiaca accessions produced abundant amounts of small seed, and this seed yield was significantly correlated with total aboveground biomass (dry weight; r 2 = 0.84, P r 2 = 0.58, P r 2 = 0.83, P F. valesiaca relatedness to other economically important Festuca species. The AFLP-based, neighbor-joining analysis differentiated F. valesiaca accessions from US Festuca cultivars examined, except for ‘Durar’ (F. ovina L.; 6x; sheep fescue), to which they had strong genetic affinities. Given their morphological attributes, F. valesiaca PI 659923, W6 30575, PI 659932, and W6 30588 should be considered for use in low-maintenance, semi-arid turf improvement programs in the western US.
    No preview · Article · Jan 2014 · Genetic Resources and Crop Evolution
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    ABSTRACT: The USDA-ARS announces the public release of the cultivar 'Stabilizer' (Reg. No. CV-33, PI 665034) Siberian wheatgrass [Agropyron fragile (Roth) Candargy] as a low-growing, rapid-establishing revegetation grass for use on rangelands and roadsides and as a grass component in fire-strip plantings in the Intermountain West, Great Basin, and northern Great Plains regions of the western United States. Selection emphasis in Stabilizer was for seedling establishment, persistence, seed production, pubescence, and reduced forage yield. During the establishment year, Stabilizer had similar numbers of seedlings per unit area, using a frequency grid, as did the cultivar 'Vavilov II' at Guernsey, WY, Dugway, UT, Beaver, UT, Fillmore, UT, and Malta, ID; and the cultivar 'Vavilov' at King Hill, ID and Spring City, UT. Stabilizer persisted as well as Vavilov II at Guernsey, WY, Fillmore, UT, and Beaver, UT but was significantly more persistent than Vavilov II at Malta, ID. Stabilizer had significantly less dry matter yield (0.25 kg plot(-1)) at Beaver, UT than Vavilov II (0.53 kg plot(-1)) and Vavilov (0.60 kg plot(-1)). Stabilizer was genetically distinct and unique with respect to the other available Siberian wheatgrass varieties.
    No preview · Article · Jan 2013 · Journal of Plant Registrations
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    ABSTRACT: Cucurbits are a group of diverse horticultural species grown worldwide. Their fruit are consumed fresh, cooked, or processed, and seeds can be eaten or used for their high quality cooking oil and protein meal. India and Southeast Asia, including China, comprise the primary and secondary centers of diversity, respectively, of cucumber (Cucumis sativus L.). India and central and southwest Asia comprise the primary center of diversity for melon (Cucumis melo L.), with China as a secondary center. Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai var. lanatus] is important throughout Asia, although its primary center of diversity is west and central Africa. European forms of melon, cucumber and watermelon were introduced to the New World multiple times. Asian varieties of these and other cucurbits [e.g., bitter (Momordica charantia L.) and luffa (Luffa cylindrical L.) gourds] have been introduced to the U.S from the late 1800s to the present. Sustainability and improvement of U.S. melon, cucumber, and watermelon varieties have been achieved through introgression of genes from their respective Asian germplasm pools for disease and pest resistance, increased genetic diversity, productivity, and quality. Resistance to Podosphaera xanthii (Castagne) Braun & Shishkoff was first found in two Indian melons. Three types of resistance to melon aphid (Aphis gossypii Glover) were found in melons from India and Korea. Cucumis hystrix from China was crossed with cucumber to create the amphidiploid (Cucumis hytivusChen and Kirkbride) through which novel genetic variation was introgressed to cucumber. Gynoecious sex expression in cucumber was derived from Japan. Asian watermelon accessions may be the source for canary yellow flesh, which adds unique nutritional and flavor content to our diet. Technological advancement and genetic improvement pioneered in Asia advanced cucurbit production worldwide. Research on the feasibility of seedless watermelon was initiated in Japan in the late 1920s. Today, seedless types account for a major share of the watermelon market. Grafting, which originated in Asia in 1920s, using disease- and pest-resistant, or cold tolerant rootstocks is essential for sustainable cucurbit production in many parts of the world, and holds great potential as an alternative to methyl bromide fumigation. For example, interspecific hybrids of Cucurbita maxima Duch. and Cucurbita moschata Duch. ex Poir., two New World species, are the most widely used rootstock for cucurbits in Japan and South Korea, and Indian bottle gourd (Lagenaria siceraria L.) introductions of Indian origin are valuable sources of germplasm for breeding multiple disease resistant rootstocks.
    No preview · Conference Paper · Aug 2012
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    Thomas Horejsi · Jack E. Staub · Claude Thomas
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    ABSTRACT: Marker assisted selection (MAS) may improve the efficiency of breeding downy mildew resistant cucumber cultivars. A study was conducted to identify random amplified polymorphic DNA (RAPD) markers linked to the downy mildew resistance gene (dm) which would be suitable for MAS. A total of 145 F3 families from two populations (55 from the WI 1983G × Straight 8 population and 90 from the Zudm1 × Straight 8 population) were evaluated over five locations in North America and Europe. Resistant and susceptible F3 families were identified and mean family resistance ratings were used to type individual F2 plants. No evidence for race differences in the pathogen (Psuedoperonospora cubensis (Berk. & Curt.) Rostow) between North America and Europe was found. Phenotypic correlations between locations ranged from 0.3 to 0.7. Of the 135 polymorphic RAPD markers identified from 960 primers, five were linked to dm - G14800, X151100, AS5800, BC5191100, and BC5261000. In the WI 1983G × Straight 8 population, G14800 was linked to dm at 16.5 cm, AS5800 at 32.8 cm, BC5191100 at 9.9 cm, and BC5261000 at 19.2 cm. In the Zudm1 ×Straight 8 population, G14800 was linked at 20.9 cm, X151100at 14.8 cm, AS5800 at 24.8 cm, and BC526_1000 at 32.9 cm. MarkersG14800 and BC5191100 were linked in repulsion to the dm allele, and X151100, AS5800, and BC5261000 were linked in coupling phase. These genetic markers may be exploited to develop an efficient MAS strategy for breeding resistant cucumber cultivars.
    Preview · Article · Apr 2012 · Euphytica
  • J.E. Staub · I.Y. Delannay

    No preview · Article · Nov 2011
  • Jack E. Staub · Isabelle Y. Delannay · Jin-Feng Chen

    No preview · Article · Oct 2011 · HortScience: a publication of the American Society for Horticultural Science
  • J.E. Staub · P.W. Simon · H.E. Cuevas

    No preview · Article · Oct 2011
  • J.E. Staub · J.D. McCreight · J.E. Zalapa

    No preview · Article · Oct 2011
  • Jack E. Staub · Isabelle Y. Delannay

    No preview · Article · Sep 2011 · HortScience: a publication of the American Society for Horticultural Science

Publication Stats

4k Citations
270.33 Total Impact Points


  • 2011-2015
    • Utah State University
      • Department of Plants, Soils and Climate
      لوگان، اوهایو, Ohio, United States
  • 1982-2015
    • University of Wisconsin–Madison
      • • Department of Horticulture
      • • Agricultural Research Service
      Madison, Wisconsin, United States
  • 2014
    • Agricultural Research Service
      ERV, Texas, United States
  • 1989-2014
    • United States Department of Agriculture
      • Agricultural Research Service (ARS)
      Washington, Washington, D.C., United States
  • 1992-2008
    • University of Wisconsin - Stout
      Menominee, Wisconsin, United States
  • 1997
    • Michigan State University
      • Department of Horticulture
      East Lansing, MI, United States
  • 1982-1984
    • Pennsylvania State University
      • Department of Plant Science
      University Park, Maryland, United States