Th Papanikolaou

University of Thessaly, Iolcus, Thessaly, Greece

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Publications (13)12.93 Total impact

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    ABSTRACT: Blood concentrations of progesterone, FSH and oestradiol in Karagouniko ewes subjected to laparoscopic ovum pick-up (OPU) at specific stages of induced oestrous cycle, were measured. Twenty-four cyclic ewes were randomly allocated into four equal groups (A, B, C and D). Oestrus was synchronized with progestagen intravaginal sponges and detected by teaser rams (oestrus: day 0). In group A, during the induced oestrous cycle, OPU was performed on days 4, 9 and 14 (sessions A1, A2 and A3, respectively). In group B and group D, OPU was performed once, on day 9 and 14, respectively. In group C (controls), endoscopic observation of follicular population was performed three times, as in group A. Starting at sponge removal, progesterone was measured in blood samples collected on 22 daily occasions and oestradiol in samples collected on 27 occasions collected at various time-points starting 2 h before to 24 h after OPU. Follicular populations did not differ among A1, A2, A3 or between C1, C2, C3 and A1, A2, A3 or A1, B, D, respectively. Oocytes of better quality (category ‘1’ or ‘2’) were collected at A3 session compared with A1 (P < 0.05). Progesterone concentration and oestrous cycle length did not differ among groups. Decreased oestradiol concentrations followed by FSH increase were recorded 3–5 h after OPU. The results confirm the regulatory role of oestradiol on FSH secretion. The quality of collected oocytes was improved in subsequent pick-up sessions in the oestrous cycle. Moreover, OPU at specific stages of the luteal phase of the cycle, even when applied repeatedly, do not affect the normal oestrous cycle length of ewes.
    Full-text · Article · May 2013 · Animal reproduction science
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    ABSTRACT: The structural stability of transcriptionally inert paternal chromatin is of vital importance for the fertilization process and early embryonic development. Accordingly, a series of eight experiments were conducted during a 7-month period to investigate: (1) effects of bull breed, individuality, successive ejaculations, semen quality characteristics (SQC), semen dilution rates and hypothermic storage of semen in a Tris-egg yolk extender on incidence of sperm nuclear chromatin instability (NCI), and (2) effects of the interaction between variation of NCI within a frozen ejaculate and variation of oocytes quality due to maturation time and/or season on the efficiency of in vitro embryo production (IVEP). Semen samples were collected once a week from six bulls using an AV and only ejaculates (n=220) of >0.30x10(9) sperm/ml and >or=60% motility were used. NCI was measured by: (1) detection of lysine-rich histones in sperm chromatin using aniline blue staining, (2) sperm susceptibility to acid-induced nuclear DNA denaturation in situ using acridine orange test, and (3) sperm susceptibility to nuclear chromatin decondensation (NCD). Bovine oocytes (n=695) were matured in vitro for 18 or 24 h, fertilized after sperm selection through a swim-up procedure and cultured for 72 h. The results showed that the 2nd ejaculates were superior to the 1st ones with respect to chromatin stability. Dilution of semen to 49.67+/-8.56x10(6) sperm/ml (1:19) decreased resistance of sperm to NCD. Cooling of semen had no significant effect on chromatin stability. Cryopreservation of semen augmented sperm vulnerability to DNA denaturation. Improvement of SQC (semen volume, sperm motility, velocity, viability and morphological normalcy) was generally concomitant with increase of sperm resistance to NCI. While Blonde d'Aquitaine bulls had a resistance to NCD higher than Limousine bulls in fresh semen, the former showed a greater susceptibility to DNA denaturation than the latter in cooled semen. Individuality significantly influenced NCI. The variability of NCI within a frozen ejaculate affected efficiency of IVEP. Significant negative correlations were observed between incidence of NCI and both fertilization rate and developmental capacity of embryos after maturation of oocytes for 18 h. The significant variation in IVEP traits due to season was independent of the effect of sperm chromatin instability.
    No preview · Article · Mar 2008 · Animal Reproduction Science
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    ABSTRACT: The structural stability of transcriptionally inert paternal chromatin is of vital importance for the fertiliza-tion process and early embryonic development. Accordingly, a series of eight experiments were conducted during a 7-month period to investigate: (1) effects of bull breed, individuality, successive ejaculations, semen quality characteristics (SQC), semen dilution rates and hypothermic storage of semen in a Tris-egg yolk extender on incidence of sperm nuclear chromatin instability (NCI), and (2) effects of the interaction between variation of NCI within a frozen ejaculate and variation of oocytes quality due to maturation time and/or season on the efficiency of in vitro embryo production (IVEP). Semen samples were collected once a week from six bulls using an AV and only ejaculates (n = 220) of >0.30 × 10 9 sperm/ml and ≥60% motility were used. NCI was measured by: (1) detection of lysine-rich histones in sperm chromatin using aniline blue staining, (2) sperm susceptibility to acid-induced nuclear DNA denaturation in situ using acridine orange test, and (3) sperm susceptibility to nuclear chromatin decondensation (NCD). Bovine oocytes (n = 695) were matured in vitro for 18 or 24 h, fertilized after sperm selection through a swim-up procedure and cultured for 72 h. The results showed that the 2nd ejaculates were superior to the 1st ones with respect to chromatin stability. Dilution of semen to 49.67 ± 8.56 × 10 6 sperm/ml (1:19) decreased resistance of sperm to NCD. Cooling of semen had no significant effect on chromatin stability. Cryopreservation of semen augmented sperm vulnerability to DNA denaturation. Improvement of SQC (semen volume, sperm motility, velocity, viability and morphological normalcy) was generally concomitant with increase of sperm resistance to NCI. While Blonde d'Aquitaine bulls had a resistance to NCD higher than Limousine bulls in fresh semen, the former showed a greater susceptibility to DNA denaturation than the latter in cooled semen. Individuality
    Full-text · Article · Jan 2008 · Animal Reproduction Science
  • I Valasi · L Leontides · Th Papanikolaou · G S Amiridis
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    ABSTRACT: Experiments were conducted to determine the effects of lamb age, frequency of follicular aspirations, and hormone stimulation by fixed or variable FSH dose, on the number of collected oocytes and their maturational competence. In trial 1, the characteristics of follicular population (number and diameter of follicles) were studied in 40 lambs which were slaughtered at the age of 30 days (S1), 42 days (S2), 60 days (S3) and 5-6 months (S4), each n = 10. In trial 2, 27 lambs were divided into four groups. group MF lambs (n = 6) had follicular aspiration (OPU) in four monthly intervals commencing from the age of 8-9 weeks (sessions MF1, MF2, MF3 and MF4). In groups SF2, SF3 and SF4 (each n = 6), OPU was conducted once during the 12-13, 16-17 and 20-21 week of age, respectively. Ovarian stimulation was conducted with fixed FSH dose (3.52 mg/animal). In trial 3, 10 lambs (group MV) were treated as those of group MF apart from the FSH dose, which was administered according to the body weight in a dose of 0.27 mg/kg. The number and the size of follicles, the number and the quality of collected oocytes and the maturational competence of the oocytes were compared between and within groups. In trial 1, the total number and the number of small follicles were greater in groups S1 and S2 compared with those of S3 and S4 (p < 0.01). Similarly, the follicular population was greater in group MF1 than in group SF3 (p < 0.01). In sessions MF2, MF3, MV2, MV3 and MV4, more oocytes were collected in comparison with those from the respective once-aspirated age mates (groups SF2, SF3 and SF4). In total, more (p = 0.02) oocytes per donor were collected from group MV (15.2 +/- 5.5) than from group MF (9.0 +/- 3.2). An absolute maturational failure was observed in oocytes collected from groups SF2 and SF3. Maturational competence varied between 16.7% and 58.3% (p = 0.017) among sessions of group MF, but it was more uniform among sessions of group MV (range 12.5-42.9%, p > 0.05). Our results indicate that firstly, the number and the quality of harvested oocytes from juvenile lambs can be much improved if follicular stimulation regime is adjusted to the body weight. Secondly, in terms of follicular population and oocyte quality, 3 and 4-month-old lambs are naturally bad oocyte donors, but this characteristic can be reversed by a previous follicular ablation.
    No preview · Article · Jun 2007 · Reproduction in Domestic Animals
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    Irene Valasi · I Menegatos · Th Papanikolaou · P Goulas · G.S. Amiridis
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    ABSTRACT: A study was designed to evaluate whether the time of onset of puberty and fertility of young ewe lambs would be affected by oocyte pick-up conducted in single or repeated sessions during the first months of lambs' live. Five groups of lambs from the Karagouniko breed were used (A-E each n=12). In group A no treatments were applied (control group) while, laparoscopical follicular aspiration (OPU) was performed early in the third, fourth and fifth month of lambs age (groups C-E, respectively). From the second to fifth month of their age, group B lambs were aspirated four times in monthly intervals. All lambs were weighed at birth, weaning, at second month and monthly thereafter until the eighth month of age. Progesterone priming and ovarian stimulation by serial FSH administrations proceeded each OPU session. To determine onset of puberty blood progesterone concentration was assayed in samples collected initially every week and after the seventh month of age twice weekly. From the seventh month a fertile ram was introduced in each group and oestrous behavior/mating was daily monitored and recorded. Pregnancy diagnosis was carried out by transabdominal ultrasound scanning 55 days after rams' removal. At the fourth and fifth month of age group B lambs were lighter (p<0.05) than controls, but this difference was later equalized. The time of onset of puberty did not differ between groups (p=0.069) and ranged between 224 and 270 days. Some animals (n=15) entered puberty with a full-length luteal phase having progesterone concentration greater than 1ng/ml, while others (n=32) exhibited one or two short luteal phases before luteal length restoration. During the first breeding season 41 animals were fertilized and maintained pregnancy to term, without noticeable differences between groups (p=0.555). During the second breeding season, all ewes were naturally served and lambed at the expected time. It is concluded that OPU in young dairy lambs does not affect the time of onset of puberty, the endocrine profile of the lambs and it does not compromise their future fertility even if it is applied at four successive months.
    Full-text · Article · Jan 2007 · Theriogenology

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  • No preview · Conference Paper · Sep 2005
  • G S Amiridis · I Valasi · I Menegatos · C Rekkas · P Goulas · T Papanikolaou · C Deligiannis
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    ABSTRACT: Possible hormonal aberrations precluding conception or maintenance of pregnancy in dairy ewes subjected to ovulation synchronisation were investigated in this study. The pituitary response to exogenous gonadotrophin-releasing hormone (GnRH) was tested at different luteal stages in 36 ewes. Oestruses were synchronised by using progestagen-impregnated sponges and the animals were randomly allotted into one of three treatment groups (A, B and C; n = 12 for each). Treatments commenced on Days 4, 9 and 14 of the new cycle (oestrus was defined as Day 0). Ewes were given two GnRH injections, 5 days before and 36 h after a prostaglandin F2+/- (PGF2+/-) injection, and the animals were inseminated 12-14 h after the second GnRH injection (modified OVSYNCH). For luteinising hormone (LH) determination blood samples were withdrawn from six ewes of each group at the time of GnRH administration, and 30, 90, 180, 270 and 360 min later. Progesterone was assayed in samples taken every other day starting from oestrus and for 17 days after the second GnRH injection, and in an additional sample collected on the day of insemination. After the first GnRH injection, the LH concentration was higher in Group C than in Groups B and A (mean +/- s.d.: 64.8 +/- 10.0 ng mL(-1), 41.3 +/- 3.7 ng mL(-1) and 24.6 +/- 9.0 ng mL(-1), respectively; P < 0.05), whereas after the second GnRH injection a uniform LH release was found in all groups. PGF2+/- caused a significant decrease in progesterone (P4) concentration in all groups; however, at artificial insemination ewes that conceived had significantly lower P4 concentration in comparison with those that failed to conceive. As early as Day 5, pregnant animals had higher P4 concentrations than non-pregnant animals. Overall, 21 animals conceived (seven, nine and five ewes from Groups A, B and C, respectively). These results indicate that the proposed protocol is equally effective in inducing a preovulatory LH surge at any stage of the luteal phase, and that elevated P4 concentration along with a delayed P4 increase should be considered as a causative factor for inability to conceive.
    No preview · Article · Jan 2005 · Reproduction Fertility and Development
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    Th. Papanikolaou · G.S. Amiridis · E. Vainas · F. Samartzi · C.A. Rekkas
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    ABSTRACT: Plasminogen activators (t-PA, u-PA) are enzymes that convert the abundant zymogen plasminogen into plasmin, a trypsin-like proteinase. Plasminogen activator activity (PAA) is regulated by plasminogen activator inhibitors, and plasmin activity is regulated by plasmin inhibitors. The activity of this proteolytic system in the follicular fluid affects ovulation, atresia and oocyte quality at the respective follicle. In this study we investigated whether the maturational stage of the follicle affects PAA, plasminogen activator inhibition against t-PA (t-PAI) or against u-PA (u-PAI) and plasmin inhibition (PI) of the follicular fluid. All parameters were assessed spectrophotometrically in samples collected ex vivo from 102 pre-pubertal (2–7 month-old) Holstein heifer follicles (G1), mid-cycle-dominant follicles of 18 adult pregnant (G2) or 18 cyclic Holstein cows (G3) and the pre-ovulatory follicles of eight adult cyclic Holstein cows (G4). One-way ANOVA followed by Duncan’s test were performed; p < 0.05 was considered significant. G4 PAA (90.83 ± 21.31 IU/ml), t-PAI (49.21 ± 9.91) and u-PAI (0.28 ± 0.02) were significantly higher than G1, G2 and G3 PAA (44.85 ± 11.5, 37.76 ± 7.72, 52.5 ± 16.28), t-PAI(15.41 ± 5.2, 13.11 ± 2.46, 15.58 ± 4.6) or u-PAI(0.12 ± 0.05, 0.13 ± 0.05, 0.15 ± 0.01). No significant difference regarding PI was detected. Our results indicate that follicular fluid PAA and t- or u-PAIcould be linked to ovulation and/or better oocyte quality, as they increase in pre-ovulatory follicles.
    Full-text · Conference Paper · Sep 2004
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    G S Amiridis · C Billinis · T Papanikolaou · V Psychas · D Kanteres

    Preview · Article · Jun 2004 · The Veterinary record

  • No preview · Article · Nov 2003 · Journal of Veterinary Pharmacology and Therapeutics

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Publication Stats

91 Citations
12.93 Total Impact Points

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Institutions

  • 2003-2013
    • University of Thessaly
      • • Τμήμα Κτηνιατρικής
      • • Κλινική Μαιευτικής και Αναπαραγωγής
      Iolcus, Thessaly, Greece