[Show abstract][Hide abstract]ABSTRACT: The present study was conducted on forty lactating Nili-Ravi buffaloes to compare the reproductive performance by treating with GnRH and PGF2α for synchronization of estrus, ovulation and pregnancy per artificial insemination with or without supplemental estradiol. All buffaloes received 25 mg PGF2α at 51 days in milk (DIM) for pre-synchronization of estrus cycles. After 13 days of PGF2α injection, buffaloes were given one of four treatments; Group A (n=10) were given an intramuscular injection of 100 μg of GnRH followed by an injection of PGF2α 7 days later and a final injection of GnRH at timed artificial insemination (AI) 48 h after the PGF2α injection. Group B (n=10) were given the same treatment as that given to Group A buffaloes but with an injection of 1 mg of estradiol 24 h after the PGF2α injection. Group C (n=10) were given an intramuscular injection of 100 μg of GnRH followed by an injection of PGF2α 7 days later and a final injection of GnRH at timed artificial insemination (AI) 72 h after the PGF2α injection. Group D (n=10) were given the same treatment as that given to Group C buffaloes but with an injection of 1 mg of estradiol 24 h after the PGF2α injection. Blood samples (10 ml) were collected from all buffaloes 7 days before and again at the first GnRH of the BuffSynch protocols, at 48 and 72 h after the PGF2α injection of the BuffSynch and at 7 days after the final GnRH of synchronization protocols. Estrus was observed in 65% (13/20) of the buffaloes after the BuffSynch72 (Groups C and D) compared with 45% (09/20) buffaloes in the BuffSynch48 (Groups Aand B). Of the buffaloes which were treated with estradiol, 70% (14/20) displayed estrus as compared to 40% (08/20) of the buffaloes which were not treated with estradiol. Irrespective of treatments, a preponderance of buffaloes observed in estrus was identified 72 h after injection of PGF2α. It is obvious that estrus expression increased after estradiol treatment however merely at 72 h or 48 h after estradiol injection. Among different treatment groups, pregnancy per AI at 40 and 68 days after artificial insemination did not differ. At 40 days after artificial insemination, pregnancy per AI rates were 45.0% for BuffSynch48 (Groups A and B) and BuffSynch72 (Groups C and D), whereas 45.0% for buffaloes with (Groups B and D) or without (Groups A and C) receiving estradiol treatment. It was concluded that lactating buffaloes exposed to presynchronized timed artificial insemination program with variable interval of proestrus before GnRH, in which artificial insemination was done at the time of final GnRH injection, practiced analogous pregnancy per artificial insemination. It is important that prolonging the period of proestrus and treating buffaloes with estradiol show estrus mainly at 72 h after PGF2α but did not increase fertility.
Full-text available · Article · Jun 2014 · Buffalo Bulletin
[Show abstract][Hide abstract]ABSTRACT: Pertaining to reported high prevalence of brucellosis in Pakistan, district Layyah on
account of having thick sheep population was selected for this study. A total of 384
sheep blood samples were collected randomly from different selected private herds
in the district, and tested through Modified Rose Bengal (mRB) test and Indirect
Enzyme Linked Immunosorbent Assay (iELISA) for the serological analysis against
the Brucella antibodies. Positive samples from these two tests were further
subjected to Competitive Enzyme Linked Immunosorbent Assay (cELISA). The
individual based seroprevalence of brucellosis in sheep was found to be 7.0% by
mRB. Herd based prevalence was 42.5%. The highest (P<0.05) seroprevalence
(16.8%) was observed in Tehsil Layyah as compared to Tehsils Karor (2.3%) and
Choubara (4.5%). Non-significant differences were recorded between breeds, age and
sex groups and also for the animals with or without history of abortion. In case of
indirect and competitive ELISA, no sheep serum sample was found to be positive.
Full-text available · Article · Oct 2013 · Pakistan Veterinary Journal
[Show abstract][Hide abstract]ABSTRACT: The current study was conducted to determine the level of some major biochemical constituents in seminal plasma of Lohi rams, and its correlation with semen physical characteristic. For this purpose six mature healthy rams were selected from the sheep herd maintained at Livestock Production and Research Institute, Bahadurnagar, Okara. All the experimental rams were kept under similar feeding and management conditions. Total 48 semen samples were collected during the breeding season (mid-autumn). Immediately after the collection samples were subjected to quantitative and qualitative sperm parameters. Seminal plasma was separated by centrifugation at 3000 × g for 20 min and samples were stored at −20 °C till the analysis. The mean concentration of Na, K, Mg, Cu, P, Zn, and total protein (TP) in seminal plasma was 222.90 mg/dl, 48.21 ppm, 10.77 mg/dl, 2.22 mg/dl, 10.26 mg/dl, 0.35 ppm and 1.87 mg/dl respectively. Statistical analysis of the data showed the significant negative correlation between biochemical constituent (Na, Mg, Cu) and sperm characteristics (ejaculated volume, mass activity, motility percentage, sperm concentration and spermatozoa abnormalities). There was significantly positive correlation between biochemical constituent (Zn, TP, P) and sperm characteristics (ejaculated volume, mass activity, motility percentage and sperm concentration). However they were negatively correlated with sperm abnormalities.
[Show abstract][Hide abstract]ABSTRACT: The present study was conducted to determine the trace mineral profile in fertile, subfertile and pregnant mares kept under different management conditions. For this purpose the blood samples were collected without anticoagulant from 100 field mares and 100 farm mares for serum separation. All animals were grouped according to their history and rectal examination. Serum manganese levels in pregnant mares were significantly (P<0.05) higher than all other mares. Serum iron levels showed no significant difference between the groups and within the groups. Pregnant mare in field conditions showed significantly (P<0.05) higher serum copper level than farm animals. Serum zinc levels in estrual group of mares under field conditions showed significantly (P<0.05) lower levels compared with rest of the three groups and from farm maintained groups. Serum zinc levels in estrual mares under farm condition were significantly (P<0.05) higher as compared to their counterparts under field conditions. Fertile, subfertile and pregnant mares under field conditions differed significantly (P<0.05) from one another, pregnant mares showed significantly (P<0.05) higher levels compared with rest of three groups under same condition. Pregnant mares under field conditions showed significantly (P<0.05) higher serum selenium levels when compared with the farm animals. It can be concluded that deficiency of manganese, iron, zinc, copper and selenium might be possible causes of infertility in mares.
[Show abstract][Hide abstract]ABSTRACT: The present study was conducted during 2005 and 2006 on 200 Nili-Ravi buffaloes kept in two agroecological zones (irrigated [zone 1] and rain-fed [zone-2]) of Punjab, Pakistan, with the objective to determine the level of trace minerals (Cu, Fe, Zn, Se) in serum of the buffaloes suffering from vaginal prolapse and to compare them with their healthy counterparts. In each zone 50 buffaloes suffering from prepartum vaginal prolapse during their seventh month of gestation were identified through survey. Vaginal prolapse-affected buffaloes belonging to zone 1 were identified as group VPB1 (N = 50), whereas buffaloes belonging to zone 2 were recognized as VPB2 (N = 50). The buffaloes of control group in zone 1 and zone 2 were identified as NCB1 and NCB2, respectively. The blood samples in all four groups of buffaloes were collected three times, i.e., first when these animals were in the eighth month of gestation, second during the eighth to ninth month of gestation, and finally when these animals were in the ninth or later month of gestation. The mean serum copper concentrations in buffaloes of group VPB1 were significantly lower (P < 0.05) in comparison with NCB1 and NCB2, whereas there were nonsignificant differences (P > 0.05) in copper concentrations between VPB1 and VPB2. There was a significant difference (P < 0.05) of iron concentration in VPB1 compared with NCB1 and NCB2. Similarly, VPB2 also had significantly lower (P < 0.05) iron concentrations compared with NCB1 and NCB2. Serum zinc concentrations were significantly lower (P < 0.05) in animals of the VPB1 group when compared with NCB1 and NCB2. Similarly, lower zinc concentrations were observed in VPB2 in comparison with NCB1 and NCB2. There was significantly lower (P < 0.05) zinc concentration in affected buffaloes (VPB1 and VPB2) from the ninth month of gestation to term when compared with those in the eighth to ninth mo of gestation, and with those not yet in the eighth month of gestation. Serum selenium concentration were significantly higher (P < 0.05) in control group buffaloes (NCB1 and NCB2) in comparison with vaginal prolapse-affected buffaloes (VPB1 and VPB2). During different stages of gestation, mean serum selenium concentrations varied nonsignificantly (P > 0.05) within each group of buffalo. Based on information obtained from this study, it was concluded that the low serum concentration of copper and selenium are linked to increased incidence of vaginal prolapse in buffaloes during the last trimester of gestation.
Full-text available · Article · Jan 2012 · Theriogenology
[Show abstract][Hide abstract]ABSTRACT: The present study was planned to evaluate the efficacy of vitamin E-selenium administration during last month of gestation in improving the productive and reproductive performance of dairy buffaloes and on growth performance of their calves. For this purpose, 40 Nili-Ravi buffaloes in their last month of pregnancy were randomly divided into two groups. The buffaloes in group 1 were treated with 10 ml of vitamin E-Se (Farvet Holland), containing 50 mg of vitamin E and 0.5 mg of sodium selenite/ml) intramuscularly on day 30 and 15 prior to expected date of parturition, while buffaloes in group II served as control. All the experimental buffaloes were kept under similar management and feeding conditions. The data on productive performance showed that the colostrum production was 22% higher (P<0.05) in treated buffaloes (14.15 3.29L) as compared to their counterparts (11.51 3.39L). The average milk production of first 90 days of lactation was 7% higher (P>0.05) in Vit E-Se treated buffaloes (706.90 314.29L) as compared to control group (663.85 225.46L). Data on reproductive performance showed that vitamin E-Se administration significantly (P<0.01) improved the conception rate (66 vs 45%) and estrus rate (90 vs 55%). The mean calf body weight at birth and at 4 weeks of age was higher by 3 and 12%, respectively, in calves born to vitamin E-Se treated buffaloes, the difference was non significant (P>0.05). It was inferred that the administration of vitamin E-Se during late gestation in buffaloes have beneficial effect on colostrum production, post partum estrus rates and conception rates.
[Show abstract][Hide abstract]ABSTRACT: The objective of present study was to know the incidence of pre partum vaginal prolapse in irrigated and rain fed agro-ecological zones of Punjab through field survey. The study was conducted, during 2005-2006, in two agro-ecological zones of Punjab, Pakistan. The area for sample collection comprised of the District of Bahawalpur from the irrigated zone (Zone-1) and the District of Muzaffargarh from the rain fed zone (Zone-2). The highest incidence of pre-partum vaginal prolapse was recorded during the months of summer and the lowest incidence was recorded in the months of spring in both the agro-ecological zones. Buffalo below the age of five years had the highest incidence of pre-partum vaginal prolapse in both the irrigated and rain fed zones. The survey revealed that the incidence of pre partum vaginal prolapse was lowest in animals fed commercially available concentrate. Animals fed cotton seed cake only had a significantly higher incidence of vaginal prolapse in both irrigated and rain fed zones. The buffalo carrying male calves had significantly higher incidence of pre partum vaginal prolapse compared with those carrying female calves in both the zones. It was concluded that the incidence of vaginal prolapse is influenced by the agro-ecological zone and the month of gestation, season of the year, lactation number, milk production, sex of calf are the contributory factors in the incidence of vaginal prolapse in buffalo in Punjab, Pakistan.
[Show abstract][Hide abstract]ABSTRACT: The low superovulatory response and poor recovery rate of oocytes are major impediments to the application of in vitro fertilization and embryo transfer technology in buffaloes. The present study aimed to evaluate the comparative efficacy of oocyte collection methods, season (low and peak breeding season), and ovarian status (presence or absence of corpus luteum) on the recovery rate and quality of recovered buffalo follicular oocytes. For this purpose riverine buffalo ovaries were collected from buffaloes slaughtered at Faisalabad Municipal Corporation Slaughter House. To study the efficiency of 3 different recovery methods the ovaries were dissected (n = 291), punctured (n = 301), or aspirated (n = 298). In all, 675 oocytes were recovered by dissection, 441 by puncture, and 363 by aspiration. The results of the present study revealed that a significantly higher (P < 0.05) oocyte recovery rate was obtained from the ovaries collected during the peak-breeding season and from those in which the corpus luteum was absent. Of the 3 oocyte recovery methods used, dissection yielded the highest percentage (36.74%) of type I oocytes, followed by puncture (32.87%), and aspiration (19.83%).
Article · Feb 2008 · Turkish Journal of Veterinary and Animal Sciences
[Show abstract][Hide abstract]ABSTRACT: The present study was conducted to determine the correlation of hypo-osmotic swelling test with conventional semen evaluation parameters of fresh semen collected from two Nili-Ravi buffalo and two Sahiwal cow bulls. A total of 10 pooled samples (each comprising two consecutive ejaculates) from each bull were collected. Each semen sample was divided into two parts. One part was used for the evaluation of semen by conventional method, while the other part was subjected to hypo-osmotic swelling test by using 150 mOsm/L sodium citrate fructose solution. The mean sperm positive to HOS test was 85.25% both in Nili-Ravi buffalo and Sahiwal cow bull semen. Statistical analysis of the data revealed a significant (P
[Show abstract][Hide abstract]ABSTRACT: The present study was conducted to determine the correlation of hypo-osmotic swelling test with conventional semen evaluation parameters of fresh semen collected from two Nili-Ravi buffalo and two Sahiwal cow bulls. A total of 10 pooled samples (each comprising two consecutive ejaculates) from each bull were collected. Each semen sample was divided into two parts. One part was used for the evaluation of semen by conventional method, while the other part was subjected to hypo-osmotic swelling test by using 150 mOsm/L sodium citrate fructose solution. The mean sperm positive to HOS test was 85.25% both in Nili-Ravi buffalo and Sahiwal cow bull semen. Statistical analysis of the data revealed a significant (P<0.05) positive correlation between progressive motility, morphologically normal spermatozoa, sperm viability and percentage of HOS test positive spermatozoa for both species. It was inferred that HOS test could be a valuable method for routine evaluation of semen for artificial insemination.
[Show abstract][Hide abstract]ABSTRACT: Jamil H., H.A. Samad, N.U. Rehman, Z.I. Qureshi, L.A. Lodhi: In vitro Maturation and Fertilization of Riverine Buffalo Follicular Oocytes in Media Supplemented with Oestrus Buffalo Serum and Hormones. Acta Vet. Brno 2007, 76: 399-404. Effects of two maturation media (TCM-199 and Ham's F-12) with and without the addition of oestrus buffalo serum (OBS) and hormones (FSH, LH, E2) on the maturation rate of buffalo follicular oocytes were evaluated. The results revealed a signifi cant (P < 0.05) increase in the maturation rate when the OBS and hormones were added to TCM-199 than in Ham's F-12 medium. The percentage of maturation rates in TCM-199 + hormones + OBS, TCM-199 + hormones, TCM-199 + OBS and TCM-199 were 77.44, 55.17, 62.28 and 26.62 percent, respectively. While in Ham's F-12 + OBS + hormone, Ham'F-12 + hormone, Ham's F-12 + OBS and Ham's F- 12 were 32.85, 27.52, 31.38 and 13.46 percent, respectively. A signifi cantly higher (P < 0.05) fertilization rate was recorded for modifi ed Ca 2+ free Tyrode's medium (63.72%) than in TALP (10.9%) and IVF-TL (32.18%). Thus, TCM-199 containing hormones and OBS appeared better for in vitro maturation, whereas modifi ed Ca 2+ free tyrode's medium was found to be more suitable for in vitro fertilization of buffalo follicular oocytes. Reproduction, Bubalus bubalis, TCM-199, Ham's F-12, FSH, LH, E2 Riverine buffaloes (Bubalus bubalis) are hardy dairy animals, resistant to climate, stress and diseases. However, problems like delayed onset of reproductive maturity, seasonality of breeding, long calving interval, latent oestrus, low number of primordial follicles and
Full-text available · Article · Sep 2007 · Acta Veterinaria Brno
[Show abstract][Hide abstract]ABSTRACT: The present study was designed to compare fertilization rates following oocyte exposure to spermatozoa from different buffalo bulls, using three sperm preparation methods i.e. percoll density gradient, swim-up (modified Ca2 free Tyrode’s medium and TALP medium) and sodium citrate washing prior to co-incubation with oocytes. Buffalo oocytes were aspirated from ovarian follicles within 1.5 to 2 hours after slaughter. They were matured in TCM-199 supplemented with 20% oestrus buffalo serum drops under mineral oil in CO2 incubator at 39C for 24 hours. Matured oocytes were transferred to the fertilization droplets and inseminated with 1x106 capacitated sperms prepared by different experimental methods. Data collected on recovered sperm motility immediately after treatment and 24 hours after incubation (at 37C) and cleavage rate of co-incubated oocytes were subjected to statistical analysis. The percentage of motile spermatozoa was significantly higher (P<0.05) in semen samples prepared by swim-up method in Ca2 free Tyrode’s medium compared to other experimental techniques. Bull wise comparison showed significantly higher (P<0.05) motility in bull B1 (50.50 ± 5.92%), followed by bull B2 (46.46 ± 5.99%) and B3 (45.96 ± 5.79%). Fertilization rate was also significantly (P<0.05) higher for spermatozoa prepared by Swim-up method in Ca2 free Tyrode’s medium (63.75 ± 2.81%), followed by sodium citrate (26.70 ± 5.08%), swim-up TALP (29.14 ± 3.74%) and Percoll gradient density (23.89 ± 3.88%). Fertilization rate was significantly higher (P<0.05) in oocytes inseminated with semen from bull B1 (43.43 ± 8.59%), followed by B2 (33.38 ± 9.95%) and B3 (30.80 ± 9.56%). The results of present study indicate that bulls and sperm preparation methods differ in their contribution to in vitro fertilization rate. Further studies are suggested to ascertain the factors responsible for such specific effects.
[Show abstract][Hide abstract]ABSTRACT: Serum glucose, total proteins, calcium and inorganic phosphorus were determined in estrual, pregnant, infertile and regular breeder mares, each group comprising of 10 animals. The mares were grouped on the basis of rectal examination, history and records. There was a significant (P<0.05) difference in the serum glucose levels between the pregnant and estrual mares, pregnant and regular breeder mares, infertile and regular breeder mares and between infertile and estrual mares. The highest values of serum glucose were recorded in regular breeder mares, whereas the lowest values were recorded in infertile mares. The highest values for total proteins were recorded in regular breeder mares and the lowest in pregnant mares, difference being significant (P<0.05) between the pregnant and estrual and pregnant and regular breeder mares. The highest values of serum calcium were recorded in estrual mares and lowest for regular breeder mares. There was a significant (P<0.05) difference between the estrual and pregnant mares, estrual and infertile mares and between estrual and regular breeder mares. The highest values of serum inorganic phosphorus were recorded for regular breeder mares whereas the lowest values were recorded in pregnant mares. The analysis of data indicated non significant (P>0.05) difference in the serum phosphorus levels among all the groups of mares.