Ping Tu

Peking University, Peping, Beijing, China

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Publications (43)91.8 Total impact

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    ABSTRACT: Objective: To evaluate the clinicopathological features of extramammary Paget's disease. Methods: Statistical analyses were performed for the clinicopathological features for 75 cases of extramammary Paget's disease from 2004 to 2013. Results: Extramammary Paget's disease exhibited a male predominance (5.25:1). The mean age of onset was (64.23±12.02) years. Single lesion accounted for 92.0%(69/75) and most of them were located in genital area. Pruritus occurred in 61.6%(45/73) patients. Invasive extramammary Paget's disease accounted for 22.7%(17/75). The concordance between clinical and pathological diagnoses was 85.3%(64/75). Among 11 misdiagnosed cases, 7 cases were misdiagnosed as eczema. Comparing Paget cells located in epithelium of adnexa or dermis and those confined to epidermis, significant differences existed in age of onset, course, recurrence rate (all P<0.05), but not in lesion size. Among 67.1%(47/70) of patients undergoing Mohs surgery, 38.3%(18/47) had a complete one-time resection while 61.7%(29/47) required more than twice for thorough resection. The 5-year recurrence rate was 25.0%. The postoperative 5-year recurrence rate of Mohs micrographic surgery (MMS) was 18.0% and the rate of non-MMS 36.5%. From 2011 to 2013, 3 recurrent cases had no radiotherapy. However another 15 cases on radiotherapy were non-recurrent. Conclusions: With a slow progression, extramammary Paget's disease is more common among elder males. Recurrence rate is associated with the degree of invasion. Thus an early diagnosis is essential. Due to obvious differences between gross and pathological boundaries, Mohs surgery may help to determine the margin. Meanwhile, adding radiotherapy reduces the recurrence rate.
    No preview · Article · Dec 2015 · Zhonghua yi xue za zhi
  • Y Zhao · C.Y. Li · C.M. Wen · Y.B. Wei · R.Y. Li · G Wang · P Tu
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    ABSTRACT: Background: Actinic keratosis (AK) is one of the most common conditions treated by dermatologists in the western countries. Studies have shown that the AK prevalence in European, American, and Australian countries is 4.5-60%. No data of AK prevalence in China has been reported. Objective: This study aimed to explore the prevalence of AK in patients visiting dermatologists in two hospitals of China. Methods: This study was conducted in the dermatology departments of two teaching hospitals. All records for five years between 2008 and 2012 with clinically or pathologically diagnosed AKs were collected from the pathological databases of both hospitals. Data from these records were used to calculate the prevalence of AKs among the patients who were seen by dermatologists in these hospitals. To estimate the reliability of data from the previous database, a cross-sectional study was conducted simultaneously in the two hospitals from Oct. 15 to Dec. 08 in 2012 after all dermatologists in the two departments were re-trained with intense courses for recognizing AK clinically. Results: The prevalence of total clinical AK through 2008-2012 was 0.52‰ in 1,590,817 patient-visits in the two hospitals. The yearly prevalence of clinical AKs were 0.30-1.20‰. In the cross-sectional study, 72,437 clinical patients were screened and 76 patients (1.05‰) were identified to have clinically recognized AK. Conclusions: The overall prevalence of AKs in the patients visiting dermatologists in the two hospitals of China was 0.52‰, which is much lower than the prevalence in the western countries. This article is protected by copyright. All rights reserved.
    No preview · Article · Dec 2015 · British Journal of Dermatology
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    Y Zhao · C.M. Wen · N.N. Zhou · Q Feng · P Tu

    Full-text · Article · Jun 2014 · Journal of the European Academy of Dermatology and Venereology
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    ABSTRACT: Mycosis fungoides (MF) often mimics the common chronic inflammatory skin diseases and is difficult to be diagnosed with certainty, partly because of the lack of well-characterized molecular markers. Previously, we discovered that TOX, a key T cell development regulator,was aberrantly over-expressed in early stage MF. In the current multi-center study involving two independent patient cohorts, we determined the prevalence of TOX over-expression in the full spectrum of MF skin biopsies, and tested if TOX expression levels correlated with long term clinical outcomes. We examined TOX expression levels in 113 MF biopsies. We found that the MF biopsies expressed higher TOX mRNA than the controls in both cohorts (17.9 fold in cohort 1, P = 0.002; 5.8 fold in cohort 2, P < 0.0001). In addition, thicker skin lesions such as plaques and tumors expressed even higher TOX levels than thinner patches. Further, TOX over-expression differentiated MF from the controls (area under the curve [AUC]=0.87, P < 0.0001). Finally, high TOX mRNA levels correlated with increased risks of disease progression (P = 0.003) and disease-specific mortality (P = 0.008). In conclusion, TOX may be a useful marker for improving MF diagnosis and prognostication.
    Full-text · Article · May 2014 · Oncotarget
  • Z X Chen · M S Zhou · Ping Tu · X Y Li · S X Yang · Y S Huang · Hang Li
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    ABSTRACT: To investigate the clinical and histopathological characteristics of basal cell carcinoma (BCC) in Chinese patients. Clinical and pathological data of BCC confirmed by histology from 2010 to 2012 in Peking University First Hospital were retrospectively analyzed. Among 418 patients enrolled, the male/female ratio was 0.77:1. The average age was (65.39±13.51) years. Among the patients younger than 60 years who occupied 29% of all the cases, the male/female ratio was 1.16:1. In terms of the histology subtypes of the BCCs, 81.8% were nodular, followed by superficial (9.8%), and the others were in very small proportion. The head and face were the most common sites of BCC (86.6%). All morpheaform subtypes, and the majority of the nodular subtypes were located on the head and face, whereas the trunk and extremities were the most common locations for the others. Clinically, 86.6% of the BCC were pigmented and 80.4% were not ulcerated. The diagnostic accordance rates of BCC on the head and face (84.7%) and on the trunk (79.1%) were higher than those on the extremities (46.2%, P<0.05). The most clinical and histopathological characteristics of our cases were similar to those of Caucasian. This study displays some unique characteristics. The young and middle aged patients occupied relative higher proportion, and their gender ration was different from that of the aged group. Tumor with hyperpigmentation was popular and few cases were ulcerated. In this study, multiple BCC cases were seldom, and the BCC patients with nevus sebaceous were older than those in other reports. The research of the diagnostic accordance rates of BCC revealed that both doctors and patients should pay more attention to BCC.
    No preview · Article · Apr 2014 · Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences
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    ABSTRACT: Cutaneous CD30+ lymphoproliferative disease (CD30+LPD), characterized by the presence of CD30+ anaplastic large T cells, comprises the second most common group of cutaneous T cell lymphoma (CTCL). However, little is known about the pathobiology of the CD30+ lymphoma cells, as well as the mechanisms of disease progression. Here we report that Special AT-rich region binding protein 1 (SATB1), a thymocyte specific chromatin organizer, is over-expressed in CD30+ lymphoma cells in most CD30+LPDs, and its expression is up-regulated during disease progression. Our findings show that SATB1 silencing in CD30+LPD cells leads to G1 cell cycle arrest mediated by p21 activation. Using chromatin immunoprecipitation, luciferase assays, and mutational analysis, we demonstrate that SATB1 directly regulates the transcription of p21 in a p53 independent manner. Moreover, DNA demethylation on a specific CpG rich region of the SATB1 promoter is associated with the up-regulation of SATB1 during disease progression. These experiments define a novel SATB1-p21 pathway in malignant CD30+ T lymphocytes, which provides novel molecular insights into the pathogenesis of CD30+LPDs and possibly leads to new therapies.
    Full-text · Article · Apr 2014 · Blood
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    Full-text · Article · Apr 2014
  • Y. Wang · X.-X. Chen · L.-S. Wu · P. Tu
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    ABSTRACT: Objective: To analyze the clinical and histopathological characteristics of cutaneous metastases of a cancer. Method: Retrospectively analyzed the clinical and pathological features, the primary sites of cancers and prognosis in 27 cases of cutaneous cancer metastases diagnosed in the department from 2006 to 2012. Results: There were 12 males and 15 females with the male to female ratio of 1:1.25. The average ages at the onset of skin lesions were aged 60 years (24-91). Nine cases (33.33%) were with single lesion and 18 cases (66.67%) with multiple lesions. The metastatic sites included the chest, abdomen, genital area, scalp, back, shoulder, and the extremities. Histopathological examination revealed that 20 cases (74.07%) were metastatic adenocarcinoma; 3 cases (11.11%) were metastatic squamous cell carcinoma; 3 cases (11.11%) were metastatic mucinous carcinoma, and 1 case (11.11%) was metastatic melanoma. Four of 16 followed up patients died, and the others continued to radiation and chemotherapy. Conclusions: With increasing incidence of cancers, cutaneous metastases increased in recent years. To raise awareness of cutaneous metastases is helpful for early diagnosis, treatment, and prolonging patients' life.
    No preview · Article · Dec 2013
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    ABSTRACT: BCL11B is a Kruppel-like C2H2-type zinc finger transcription factor, which has been associated with several human malignancies. Recent evidence showed that overexpressed BCL11B conferred chemoresistance to malignant T cells and that inhibiting BCL11B led to increased apoptosis, suggesting its potential pathogenic relevance in cutaneous T-cell lymphomas (CTCL), which were characterized by the resistance to chemotherapy-induced apoptosis. To investigate the expression pattern of BCL11B in different stages of mycosis fungoides (MF), quantitative reverse transcription polymerase chain reaction and immunohistochemistry were performed to compare the mRNA and protein expression among different stages of MF and benign inflammatory dermatoses (BID), respectively. BCL11B demonstrated significant upregulation in all stages of MF, compared with BID, in both mRNA expression level and protein level. In addition, BCL11B expression increased with advancing lesion tumor stage and overall disease stage. Further, to evaluate the dynamic expression of BCL11B under CTCL-directed treatment, BCL11B expression and cell apoptosis were evaluated after interferon (IFN)-α-2b and methotrexate treatment on CTCL cell line Hut78 cells. IFN-α-2b, but not methotrexate, induced BCL11B inhibition and cell apoptosis, suggesting that BCL11B may play important roles in the anti-CTCL effect of IFN-α-2b. In conclusion, our study demonstrated the overexpression of BCL11B in MF lesions and its potential relevance to disease progression. In addition, we provided evidence for BCL11B inhibitory approaches as a potential treatment to target chemoresistant tumor cells in advanced MF.
    Full-text · Article · May 2013 · The Journal of Dermatology
  • Yi Zhao · Jining Tao · Ping Tu
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    ABSTRACT: Methods for quantitatively evaluate the efficacy of photodynamic therapy (PDT) for port-wine stains (PWS) are still needed for clinical practice and studies. 50 pairs of pictures before and after PDT of 40 PWS patients were selected. Each PWS lesion was labeled with a marker of red color graded 0-9. These pictures were taken without assistance of instruments to keep same position or distance and were referred to as 'experimental' images. 70 labels were photographed at a fixed position and distance with the assistance of a bracket and the images obtained were referred to as 'standardized' ones. An independent group of three experts viewed the photos and assessed the efficacy. The images were processed and measured for erythema index (EI) with the ImageJ freeware. The EI difference (ΔEI) and the percent change of ΔEI (ratioΔEI(%)) of the labels and the PWS lesions was computed separately. Significant differences of EI, ΔEI and ratioΔEI(%) were found between each two grades of the color markers in both standardized and experimental images. ΔEI of lesions achieved 'almost cured' and 'great improvement' after PDT was significantly reduced than before PDT. Significantly greater percentages of lesions were assessed as 'response' and 'significant response' in those of beforeΔEI≥35 compared to those of beforeΔEI<35. The ratioΔEI(%) decrease of lesions assessed as 'almost cured', 'great improvement' and 'some improvement' was significantly reduced sequentially. The EI image analysis is a valid method for quantitatively evaluating efficacy of PDT for PWS.
    No preview · Article · May 2013 · Photodiagnosis and photodynamic therapy
  • Yang Wang · Jin Yu · Ping Tu

    No preview · Article · Dec 2012 · The Journal of Dermatology
  • Yang Wang · Haizhen Yang · Ping Tu

    No preview · Article · Nov 2012
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    ABSTRACT: Background The epidemiological and clinical characterization data of skin malignancies and premalignancies in Chinese population is scarce and inadequate. Objective To systematically investigate the clinical features and the trend of skin malignancies and premalignancies in 1420 Chinese cases. Methods A total of 1398 patients (presenting 1420 skin tumours) were included. Clinical and demographic information for every individual was collected, including age, age of onset, sex, lesion location, disease duration and tumour histology, which was analyzed for each type of skin tumours. Results The number of skin malignancies and premalignancies increased over time, with Basal cell carcinoma (BCC) as the most common type (30.5%). The majority of the patients were above 60 years of age both at onset and at diagnosis (52.8% and 62.9%, respectively), yet around one-third of patients were between 35–59 years (35.3% and 31.2%, respectively). Skin malignancies and premalignancies were mainly located in the head and neck (58.6%), followed by the trunk (18.3%) and the extremities (15.0%). Of all BCCs, nodular BCC was the most common histologic subtype (62.8%), while 15.8% were classified as aggressive subtypes. Malignant melanoma (MM) comprised the lowest proportion of 3.7%, with 75% located on extremities. The diagnostic accordance rates varied from 49.5% to 90.4%, with BCC being 67.9%. Conclusions The clinical features of skin malignancies and premalignancies in this study showed some similarities with those observed in Caucasian and other Asian populations, with several distinguished features in Chinese patients also being recognized. Closer attention to suspicious lesions in young and middle-aged people is needed.
    No preview · Article · Aug 2012 · Journal of the European Academy of Dermatology and Venereology
  • W.-W. Li · H.-Z. Yang · L.-S. Wu · P. Tu
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    ABSTRACT: A case of mycosis fungoides presenting as persistent pigmented purpuric dermatosis is reported. A 48-year-old male presented with eruption on his lower extremities for 3 years, the lesions gradually aggravated and with occasionally itching. Dermatological examination disclosed erythematous and red-brown petechial patches on his waist, buttocks and lower extremities, and some dark brown pigmented patches on the dorsa of feet and hands with mild lichenification. Histopathological examination showed there was focal lymphocytic infiltration in the papillary and reticular dermis, and some lymphocytes showed atypical and significant epidermotropism. Immunohistochemical investigation showed tumor cells stained positive for CD3, CD5, CD4, CD8, TCRβ, and TIA1, and negative for CD20. Thus mycosis fungoides was diagnosed.
    No preview · Article · May 2012
  • B. Zhang · W. Chen · Y. Yang · Y. Wang · W.-W. Li · R.-Y. Li · P. Tu
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    ABSTRACT: A case of Multi-centric reticulohistiocytosis is reported. A 56-year-old male patient with papules, nodules and dark red plagues with edema on his face, trunk and extremities for more than 1 year, stiffness and deformity of the fingers, limitation of shoulder and knee joint activity, debility of the limbs for about 10 months. Radiography of the knees revealed destruction of joints and part of the articular facet had cystic lower density zones. Histopathological examination showed many single nucleated cells and multi-nucleated giant cells with ground-glass cytoplasm in the dermis and among the muscle cells. The final diagnosis was multi-centric reticulohistiocytosis.
    No preview · Article · Mar 2012
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    ABSTRACT: Olmsted syndrome (OS) is a rare congenital disorder characterized by palmoplantar and periorificial keratoderma, alopecia in most cases, and severe itching. The genetic basis for OS remained unidentified. Using whole-exome sequencing of case-parents trios, we have identified a de novo missense mutation in TRPV3 that produces p.Gly573Ser in an individual with OS. Nucleotide sequencing of five additional affected individuals also revealed missense mutations in TRPV3 (which produced p.Gly573Ser in three cases and p.Gly573Cys and p.Trp692Gly in one case each). Encoding a transient receptor potential vanilloid-3 cation channel, TRPV3 is primarily expressed in the skin, hair follicles, brain, and spinal cord. In transfected HEK293 cells expressing TRPV3 mutants, much larger inward currents were recorded, probably because of the constitutive opening of the mutants. These gain-of-function mutations might lead to elevated apoptosis of keratinocytes and consequent skin hyperkeratosis in the affected individuals. Our findings suggest that TRPV3 plays essential roles in skin keratinization, hair growth, and possibly itching sensation in humans and selectively targeting TRPV3 could provide therapeutic potential for keratinization or itching-related skin disorders.
    Full-text · Article · Mar 2012 · The American Journal of Human Genetics
  • Y.S. Huang · D F Bu · X.Y. Li · Z H Ma · Y Yang · Z M Lin · F M Lu · P Tu · H Li
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    ABSTRACT: Background Alterations of the PTCH1 gene have been found to contribute to both familial and sporadic basal cell carcinoma (BCC), especially in Caucasian patients. Furthermore, the majority of PTCH1 gene mutations in sporadic BCCs in Caucasian patients carry ultraviolet (UV) signatures, suggesting the key role of UV light in BCC development. However, sporadic BCC in non-Caucasian population has a lower incidence, and the pathogenesis remains largely unknown. To date, there has been no mutation analysis on PTCH1 gene in Chinese patients with sporadic BCCs. Objective To investigate genetic alterations of the PTCH1 gene in Chinese sporadic BCCs. Methods Direct sequencing was used to screen for mutations in PTCH1 in 31 microdissected samples in Chinese sporadic BCCs. In addition, single nucleotide polymorphisms (SNPs) were studied for loss of heterozygosity (LOH). Results Nineteen PTCH1 mutations in 17 of the 31 BCCs (54.8%) were identified. SNP analysis revealed LOH of PTCH1 in 10 of 23 BCCs (43.5%). Interestingly, the majority of mutations identified (63.2%) were insertion/deletion, which was different from the results in Caucasian cases whose mutations are predominantly point mutations. Only two (10.5%) of the remaining seven mutations were UV-specific C → T transition or tandem CC → TT transitions. All mutations occurred evenly throughout the entire PTCH1 protein domain without a hot-spot detected. Conclusion Mutations and LOH in PTCH1 were also highly prevalent in Chinese sporadic BCCs. However, UV light plays a less role in causing these mutations, suggesting other potential mechanisms in the development of sporadic BCC in Chinese patients.
    No preview · Article · Feb 2012 · Journal of the European Academy of Dermatology and Venereology
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    ABSTRACT: Sézary syndrome (SS) is an aggressive subtype of cutaneous T-cell lymphoma that is characterized by circulating leukemic Sézary cells. The accumulation of these malignant cells has been shown to be the result of the resistance to apoptosis, in particular, activation-induced cell death. However, the mechanism of apoptosis resistance remains unknown. By characterizing the gene transcription profiles of purified CD4(+)CD7(-) Sézary cells from patients with SS and cultured Sézary cells, it was found that Sézary cells are deficient in the expression of special AT-rich region binding protein 1 (SATB1), a key regulator of T-cell development and maturation. Retrovirus-mediated gene transduction revealed that SATB1 restoration in cultured Sézary cells (Hut78) triggered spontaneous cell death and sensitized Hut78 cells to activation-induced cell death, with associated activation of caspase 8 and caspase 3. Furthermore, endogenous expression of FasL in Sézary cells was increased in transcriptional and translational levels on restoration of SATB1 expression in cultured Sézary cells. These results suggest that deficiency in SATB1 expression in Sézary cells plays an important role in SS pathogenesis by causing apoptosis resistance. Thus, restoration of SATB1 expression may represent a potential molecular targeted therapy for SS, which does not have a cure at present.
    Full-text · Article · Apr 2011 · Blood
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    ABSTRACT: This phase IIa study aimed to study the efficacy and safety of hemoporfin in photodynamic therapy (PDT) with a 532 nm continuous laser for port-wine stain (PWS). In this 8-week open-labeled study in three centers, three different laser exposure times (532 nm continuous laser for 20, 30 and 40 min) were used in stage I, group A, stage II, group B and stage III, group C, respectively. Primary efficacy assessment was performed by an independent group of experts, who reviewed the standardized photos. Secondary efficacy assessment consisted of the subjective grading of the PWS fading by the investigators and the patients. Treatment reactions and adverse events (AE) were recorded separately. Forty patients were initially enrolled in the study, but stage III had to be cancelled eventually for the safety of the patients. Patients in groups A and B showed similar satisfactory results in efficacy assessments, the total 'response' rate being 80.0% and 94.7% in groups A and B, respectively. The AE rates were also similar in the two groups. Self-limiting photosensitive dermatitis and hyperpigmentation were the most frequently observed AE. Hemoporfin-PDT is effective and safe for patients with PWS aged 16-50.
    No preview · Article · Feb 2011 · Photodermatology Photoimmunology and Photomedicine
  • F. Zhang · P. Tu · D.-F. Bu · L.-S. Wu
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    ABSTRACT: Objective: To investigate the feasibility of laser capture microdissection (LCM) and genescan in T cell receptor gamma gene rearrangement in early mycosis fungoides. Methods: LCM to obtain lymphocytic infiltrates from tissue sections of skin biopsy specimens was used. T cell receptor gamma-chain polymerase chain reaction genescan analysis (TCR-γ-PCR-GSA) was used to analyze the clonality status of 9 patients with early MF and 2 patients with tumor-stage MF. Results: A monoclonal T cell infiltrate was demonstrated in 66.7% of early stage MF cases and in 100% of tumor stage MF patients. Conclusion: Applying LCM to clonality analysis of early MF could be more rapid and sensitive than conventional methods, and to introduce this approach into the routine diagnosis is recommended.
    No preview · Article · May 2010

Publication Stats

237 Citations
91.80 Total Impact Points

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  • 2005-2014
    • Peking University
      Peping, Beijing, China
  • 2002-2012
    • Beijing Medical University
      • Department of Dermatology
      Peping, Beijing, China