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Stability of SARS Coronavirus in Human Specimens and Environment and Its Sensitivity to Heating and UV Irradiation

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Abstract

The causal agent for SARS is considered as a novel coronavirus that has never been described both in human and animals previously. The stability of SARS coronavirus in human specimens and in environments was studied. Using a SARS coronavirus strain CoV-P9, which was isolated from pharyngeal swab of a probable SARS case in Beijing, its stability in mimic human specimens and in mimic environment including surfaces of commonly used materials or in household conditions, as well as its resistance to temperature and UV irradiation were analyzed. A total of 10(6) TCID50 viruses were placed in each tested condition, and changes of the viral infectivity in samples after treatments were measured by evaluating cytopathic effect (CPE) in cell line Vero-E6 at 48 h after infection. The results showed that SARS coronavirus in the testing condition could survive in serum, 1:20 diluted sputum and feces for at least 96 h, whereas it could remain alive in urine for at least 72 h with a low level of infectivity. The survival abilities on the surfaces of eight different materials and in water were quite comparable, revealing reduction of infectivity after 72 to 96 h exposure. Viruses stayed stable at 4 degrees C, at room temperature (20 degrees C) and at 37 degrees C for at least 2 h without remarkable change in the infectious ability in cells, but were converted to be non-infectious after 90-, 60- and 30-min exposure at 56 degrees C, at 67 degrees C and at 75 degrees C, respectively. Irradiation of UV for 60 min on the virus in culture medium resulted in the destruction of viral infectivity at an undetectable level. The survival ability of SARS coronavirus in human specimens and in environments seems to be relatively strong. Heating and UV irradiation can efficiently eliminate the viral infectivity.
... Se sabe que el coronavirus puede permanecer estable solamente en productos refrigerados (4 °C) o congelados (-10 °C a -80 °C) hasta 21 días (Han et al., 2021) pero no hay registros del virus en productos alimenticios por encima de esa temperatura. Es posible que los rayos ultravioletas, altas temperaturas y humedad del ambiente ayuden a inactivar al coronavirus (Duan et al., 2003;Biryukov et al., 2020), es por ello que no se encuentran cargas virales suficientes en lugares públicos (Zedwitz-Liebenstein, 2022), así como en objetos manipulables como en billetes y tarjetas de crédito (Newey et al., 2022). Al inicio de la pandemia se especuló que el coronavirus estaría en superficies de varios objetos del ambiente (Suman et al., 2020), pero estos fueron encontrados por horas o días, solamente bajo ambientes experimentales, en oscuridad y ausencia de luz ultravioleta (Ridell et al., 2020). ...
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