Effects of hyaluronic acid on peripheral nerve scarring and regeneration in rats
Division of Hand Surgery, Department of Plastic and Reconstructive Surgery, Uludağ University Medical School, Görükle, Bursa, Turkey. Microsurgery
(Impact Factor: 2.42).
01/2003; 23(6):575-81. DOI: 10.1002/micr.10209
The purpose of this experimental study was to investigate the effects of topical applications of hyaluronic acid on peripheral nerve scarring and regeneration in an adult rat model. After the right sciatic nerves of 48 rats were transected and immediately repaired, nerves were randomly divided into two groups. Nerves to which were applied hyaluronic acid comprised the experimental group, and nerves to which were applied saline comprised the control group. Perineural scarring was evaluated at 4 and 12 weeks macroscopically and histologically. Nerves treated with hyaluronic acid demonstrated significant reduction in perineural scar thickness (P < 0.05, Student's t-test). Histomorphologic nerve analysis, electrophysiologic studies, muscle mass evaluation, and serial functional walking-track analysis were performed for evaluation of peripheral nerve regeneration at 12 weeks. The results showed better conduction velocities, increased axon-fiber diameter, and faster functional recovery in hyaluronic acid-treated nerves (P < 0.05, Student's t-test). In conclusion, hyaluronic acid appears to be effective in preventing perineural scar formation, resulting in enhancement of peripheral nerve regeneration.
Available from: Agon Mekaj
- "The rabbits were allowed unrestricted movement immediately after anesthesia. All of the surgical procedures were performed using microsurgical instruments under a loupe at 3.5× magnification . "
Available from: Abit Aktaş
- "The whole sciatic nerve, along with the tissue attached to it (including the mended part), was severed, fixed in 10% formalin, embedded with paraffin, cut into 5-µm-thick transverse sections , and stained with Masson-Trichrome for evaluation of epineural fibrosis. The epineural scar tissue formation index was obtained by dividing the thickness of the scar tissue by how thick the nerve tissue was found to be (Petersen et al., 1996; Ozgenel, 2003; Ozay et al., 2007). "
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ABSTRACT: Glioblastoma cyst fluid contains growth factors and extracellular matrix proteins which are known as neurotrophic and neurite-promoting agents. Therefore, we hypothesized that glioblastoma cyst fluid can promote the regeneration of injured peripheral nerves. To validate this hypothesis, we transected rat sciatic nerve, performed epineural anastomosis, and wrapped the injured sciatic nerve with glioblastoma cyst fluid-or saline-soaked gelatin sponges. Neurological function and histomorphological examinations showed that compared with the rats receiving local saline treatment, those receiving local glioblastoma cyst fluid treatment had better sciatic nerve function, fewer scars, greater axon area, counts and diameter as well as fiber diameter. These findings suggest that glioblastoma cyst fluid can promote the regeneration of injured sciatic nerve and has the potential for future clinical application in patients with peripheral nerve injury. © 2015, Editorial Board of Neural Regeneration Research. All rights reserved.
- "HA has a positive influence on cell proliferation, attachment, and migration.   HA prevents perineural scarring and results in enhanced peripheral nerve regeneration. Covalent crosslinking of HA improves its elastic moduli. "
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ABSTRACT: Abstract Recently alginate-based tissue repair scaffolds fabricated using 3D printing techniques have been extensively examined for use in tissue engineering applications. However, their physical and mechanical properties are unfavorable for many tissue engineering applications because these properties are poorly controlled during the fabrication process. Some improvement of alginate gel properties can be realized by addition of hyaluronic acid (HA), and this may also improve the ability of cells to interact with the gel. Here we report improvement of the physical properties of alginate-HA gel scaffolds by the addition of the polycation polyethyleneimine (PEI) during the fabrication process in order to stabilize alginate molecular structure through the formation of a polyelectrolyte complex. We find that PEI has a significant beneficial influence on alginate-HA scaffold physical properties, including a reduction in the degree of gel swelling, a reduction in scaffold degradation rate, and an increase in the Young's modulus of the gel. Further study shows that fabrication of alginate-HA gels with PEI increases the encapsulation efficiency of bovine serum albumin, a model protein, and reduces the subsequent initial protein release rate. However, it was also found that survival of Schwann cells or ATDC-5 chondrogenic cells encapsulated during the scaffold fabrication process was modestly reduced with increasing PEI concentration. This study illustrates that the use of PEI during scaffold fabrication by plotting can provide an effective means to control alginate-based scaffold properties for tissue engineering applications, but that the many effects of PEI must be balanced for optimal outcomes in different situations.
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