Genome size variation among accessions of A. thaliana

Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, D-06466 Gatersleben, Germany.
Annals of Botany (Impact Factor: 3.65). 04/2004; 93(3):317-21. DOI: 10.1093/aob/mch037
Source: PubMed


Estimates of the amount of nuclear DNA of Arabidopsis thaliana, known to be among the lowest within angiosperms, vary considerably. This study aimed to determine genome size of a range of accessions from throughout the entire Eurasian range of the species.
Twenty accessions from all over Europe and one from Japan were examined using flow cytometry.
Significant differences in mean C-values were detected over a 1.1-fold range. Mean haploid (1C) genome size was 0.215 pg (211 Mbp) for all analysed accessions. Two accessions were tetraploid.
A closer investigation of the DNA fractions involved in intraspecific genome size differences in this experimentally accessible species may provide information on the factors involved in stability and evolution of genome sizes.

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    • "In contrast to intraspecific stability, numerous studies in plants have reported large variations in genome size among species within genera (Schmuths et al. 2004;Wang et al. 2009;Díez et al. 2013). Retro-elements and polyploidization are considered to be primary factors leading to the variations in genome size (Piegu et al. 2006;Grover and Wendel 2010). "

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    • "It has been correlated with several environmental traits, affecting the plant life-histories (Bennett 1972; Nandini and Murray 1997; Price and Bachmann 1975), phenology (Baranyi and Greilhuber 1999; Grime and Mowforth 1982) and plant species distribution (Bennett 1976; Bottini et al. 2000; Knight and Ackerly 2002; Levin and Funderburg 1979; Mac Gillivray and Grime 1995; Ohri and Khoshoo 1986; Poggio et al. 1989, 1998; Wakamiya et al. 1993). Moreover, correlations between DNA variation and geographic or climatic parameters were suggested for many plant species (Bennett 1995; Caceres et al. 1998; Rayburn and Auger 1990; Schmuths et al. 2004; Temsch and Greilhuber 2000, 2001). Considering Lathyrus species, the DNA content variation observed in a few species of the Northern Hemisphere was associated to the life cycles, breeding systems (Nandini and Murray 1997; Rees and Hazarika 1969; Rees and Narayan 1988) and to the frequency of chiasmata in meiotic cells (Narayan and McIntre 1989). "
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    • "This result suggests that the contigs were mapped to the same A. thaliana genes because they are homologs rather than because they are partial sequences derived from different regions of the same genes by incomplete assembly. This is consistent with Dipterocarpaceae having a larger genome size than A. thaliana (Ohri & Kumar 1986; Schmuths et al. 2004) and thus possibly more genes (Hou & Lin 2009). Therefore , we treated these contigs as different genes in the following analyses. "
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