Our aim was to determine the most effective treatment parameters for laser-assisted hair removal using long-pulsed Nd:YAG-lasers. 42 volunteers were treated with 1064 nm long-pulsed Nd:YAG-lasers. We used two different laser systems (Lyra XP, Wavelight and Smartepil II Deka-LMS) with various pulse lengths, fluences, and spot sizes. For each parameter, five test areas were treated one to five times at 4-week intervals. Follow-ups were performed 1, 3, 6, and 12 months after the last treatment. Percentual hair loss, side effects, and pain during treatment were evaluated. The average hair reduction 12 months after the last treatment using the Lyra XP was 48%, using the Smartepil II it ranged between 30% and 35%. There were no permanent side effects. Long-pulsed 1064 nm Nd:YAG-lasers are safe and effective for hair reduction. Although the different treatment parameters did not lead to a lot of differing hair reduction 12 months after the last treatment, the laser with the largest spot size and the longest pulse time showed the best results.