Elevated SNAP-25 is associated with fatty acid-induced impairment of mouse islet function

ArticleinBiochemical and Biophysical Research Communications 317(2):472-7 · May 2004with3 Reads
Impact Factor: 2.30 · DOI: 10.1016/j.bbrc.2004.03.067 · Source: PubMed

    Abstract

    The role of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins in insulin secretion following chronic exposure to non-esterified fatty acids (NEFAs) has not been extensively investigated. Here, we show that synaptosome-associated protein of 25 kDa (SNAP-25) levels were predominantly elevated in the soluble fraction of mouse islets exposed to palmitate. This coincided with an impairment of insulin secretion to glucose and non-glucose secretagogues, consistent with a defect at a distal regulatory step in exocytosis. Removal of palmitate from the media restored both SNAP-25 protein levels and insulin secretion to control levels. We conclude that increased expression of SNAP-25 is associated with NEFA-induced impairment of insulin secretion in mouse islets.