Microarray detection of E2F pathway activation and other targets in multiple sclerosis peripheral blood mononuclear cells

ArticleinJournal of Neuroimmunology 150(1-2):163-77 · June 2004with5 Reads
Impact Factor: 2.47 · DOI: 10.1016/j.jneuroim.2004.01.017 · Source: PubMed


    We performed microarray analysis of peripheral blood mononuclear cells (PBMCs) from multiple sclerosis (MS) patients and detected a profile of immune cell activation, autoantigen upregulation, and enhanced E2F pathway transcription. Accordingly, E2f1-deficient mice manifested only mild disability upon induction of experimental autoimmune encephalomyelitis (EAE). Furthermore, PBMCs from Avonex-treated patients had lower expression of E2F targets. The profile was enriched in genes known to harbor MS-associated polymorphisms, or localized to MS susceptibility chromosomal regions. Our study shows that PBMC microarrays reflect MS pathobiology that can be validated in the EAE model.