Biochemical and histological responses in mussel (Mytilus edulis) exposed to North Sea oil and to a mixture of North Sea oil and alkylphenols

Université Bordeaux 1, Talence, Aquitaine, France
Marine Environmental Research (Impact Factor: 2.76). 08/2004; 58(2-5):437-41. DOI: 10.1016/j.marenvres.2004.03.121
Source: PubMed


Several environmental chemicals are suspected to be responsible for adverse health effects on the reproductive system in various organisms. During this work, environmentally relevant concentrations of North Sea oil were used alone or in combination with alkylphenols and additional PAH to study the effect on vitellogenin-like protein expression and gonadal development in mussels. North Sea oil (0.5 ppm) induced the expression of phospho-proteins in both sexes indicating that some compounds are oestrogen-mimics. This induction was not seen in samples dosed with the mixture but signs of toxic effects were observed in the gonads. Indeed, numerous degenerating ovarian follicles in females and foci, similar to vertebrate melanomacrophage centres, were observed in testes.

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Available from: Peter-Diedrich Hansen, Nov 08, 2015
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    • "Several characteristics such as filter feeding, sessile life form and ability to accumulate pollutants in addition to their wide distribution, makes them favored organisms for estimating environmental pollution levels [5] [6]. With a range of physiological, histological and molecular responses, including abnormal morphology , alterations of anti-oxidative status, induction of DNA strand-breaks, etc., they have been shown to be applicable for in situ and ex situ assessment of the effects of pollutants present in environment [7] [8] [9]. Most importantly, they are widely employed for assessing genotoxicity [10] [11] [12] [13]. "
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    • "An heterosynthesis merges also from the assay of alkali-labile phosphate (ALP), an indirect technique utilized to determine the presence of VTG-like proteins (Blaise et al., 1999; Pampanin et al., 2005; Ricciardi et al., 2008). Indeed after ALP, the presence of vitelline-like proteins was also reported in the hemolymph of several molluscs treated with estrogen (Blaise et al., 1999; Gagné et al., 2001) or exposed to environmental contaminants (endocrine-disrupting chemicals) known to induce changes and alterations of the endocrine functions (Blaise et al., 1999; Gagné et al., 2002; Verslycke et al., 2002; Aarab et al., 2004, 2006; Marin and Matozzo, 2004; Quinn et al., 2004; Versonnen et al., 2004; Matozzo and Marin, 2005; Pampanin et al., 2005; Ortiz-Zarragoitia and Cajaraville, 2006; Zorita et al., 2006; Matozzo et al., 2008). "
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    ABSTRACT: The aim of this study is to assess, by RT-PCR, in situ hybridization, electron microscopy, and immunohistochemistry, the site/s of vitellogenin (VTG) synthesis in the mussel Mytilus galloprovincialis. Our investigations demonstrate that, among the analyzed tissues, the synthesis of VTG occurs only in the female gonad, i.e. within the oocyte and follicle and connective cells. Such a synthesis is just evident in early vitellogenic oocytes, whose cytoplasm is characterized by numerous RER cisternae and an extended Golgi complex surrounded by nascent yolk platelets. The synthesis of VTG goes on in vitellogenic oocytes assuming a pear form, and progressively reduces once the oocyte shows the pear or polygonal form, typical of those oocytes that have concluded the growth. The expression of VTG occurs also within follicle (auxiliary) and connective cells. In particular, it is noteworthy that follicle cells are characterized by numerous RER cisternae and an active Golgi complex surrounded by numerous vesicles and vacuoles containing electron dense material. The same material is also present along their plasma membrane, within the intercellular space between oocyte and follicle cells, and finally within invaginations of the oocyte surface, thus suggesting a VTG transfer to the oocyte via endocytosis. Differently, no VTG synthesis was observed within digestive gland. All together the findings here reported strongly suggest that in Mytilus galloprovincialis, inside the gonad, the VTG synthesis occurs in the oocyte (autosynthesis) and in the follicle and adipogranular cells (heterosynthesis). J. Cell. Physiol. © 2012 Wiley Periodicals, Inc.
    Full-text · Article · Mar 2013 · Journal of Cellular Physiology
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    • "Regarding the use of VTG as a biomarker in invertebrates, studies on the effect of hydrocarbons on protein-like-vitellogenin are restricted to the bivalve mollusk Mytilus edulis exposed to crude oil (Ortiz-Zarragoitia and Cajaraville, 2006; Aarab et al., 2004) where the amount of vitellogenin was determined using alkali-labile phosphate, an indirect marker of vitellin. Though this assay is considered as an alternative assessment of ELISA, this method should be applied with caution as alkali-labile phosphorus is a minor part of the total phosphorus pool in crustacean vitellogenin (Volz et al., 2002), and the presence of other phosphorus sources can substantially affect the results (Stanton, 1968). "
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