Chromatographic separation of chlorophenoxy acid herbicides and their radiolytic degradation products in water samples

Department of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland.
Water Research (Impact Factor: 5.53). 06/2004; 38(14-15):3259-64. DOI: 10.1016/j.watres.2004.03.032
Source: PubMed


HPLC procedure for simultaneous determination of chlorophenoxy acid herbicides and their radiolytic degradation products in waters is described with the use of octadecylsilica column and spectrophotometric detection at 280 nm. The satisfactory separation was achieved with a mobile phase of pH 2.5 consisting of 43.7 mM acetic acid with 40% (v/v) acetonitrile. Limit of detection values for herbicides and phenol derivatives were in the range of 19-41 microg/l and 10-60 microg/l, respectively. The developed method was applied for monitoring the effectiveness of radiolytic degradation of herbicides. Studies of products of gamma-radiolysis of 2,4-dichlorophenol have shown that the efficiency of this process is affected by the presence of naturally occurring scavengers of gamma-radiation such as carbonates or nitrates.

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Available from: Przemysław Drzewicz
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    • "Chlorophenoxy herbicides are used worldwide as plant growth regulators for agricultural and nonagricultural purposes [15] [16]. These herbicides can be easily transferred to surface and ground waters due to their polar nature and relatively good solubility which increase the risks of contamination and consequently the environmental damage [17]. Based on epidemiologic studies, phenoxy acid herbicides have been classified by the International Agency for Research on Cancer (IARC) as possibly carcinogenic to humans (category 2B) [18]. "
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    • "The use of ionizing radiation as the most efficient source of active free radicals can be especially useful for this purpose (Cooper et al., 1998). Several methods have been developed for degradation of pesticides (Angelini et al., 2000; Dessouki et al., 1999; Drzewicz et al., 2004a; Drzewicz et al., 2005; Jankowska et al., 2004; Leitner et al., 1999; Zona and Solar, 2003; Zona et al., 2002a, b), including chlorophenoxy herbicides (Drzewicz et al., 2004b; Jankowska et al., 2004; Zona and Solar, 2003; Zona et al., 2002a, b). So far, no studies have been reported of the use of this methodology for removal of MCPA from surface waters or industrial wastes. "
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