Article

Detergent protease

Henkel, Enzyme Technology, Henkelstrasse 67 40191, Duesseldorf, Germany.
Current Opinion in Biotechnology (Impact Factor: 7.12). 09/2004; 15(4):330-4. DOI: 10.1016/j.copbio.2004.06.005
Source: PubMed

ABSTRACT

Over the past 20 years, the development of subtilisins as typical detergent proteases has employed all the tools of enzyme technology, resulting in a constant flow of new and improved enzymes. The number of molecules identified and characterized, however, is in clear opposition to the number of molecules that are entering the market. Will the next-generation detergent proteases be based on new backbones different from subtilisins, or will the use of all available technologies (rational design, directed evolution and exploitation of natural diversity) yield improved subtilisins, ending the current era dominated by high alkaline subtilisins? These questions will have to be answered not only by the performance of the molecules themselves, but also by their yield in fermentation and their compatibility with existing production technologies.

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Available from: Karl-Heinz Maurer
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    • "Alkaline and high-alkaline proteases are the most prominent detergent enzymes and contribute – alone or in combination with α-amylases – to the basic performance of modern detergents. Such proteases already reached in 2002 an annual tonnage of about 900 metric tons equivalent of pure enzyme for the European market, tendency increasing [3]. "
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    ABSTRACT: Since volatile and rising cost factors such as energy, raw materials and market competitiveness have a significant impact on the economic efficiency of biotechnological bulk productions, industrial processes need to be steadily improved and optimized. Thereby the current production hosts can undergo various limitations. To overcome those limitations and in addition increase the diversity of available production hosts for future applications, we suggest a Production Strain Blueprinting (PSB) strategy to develop new production systems in a reduced time lapse in contrast to a development from scratch.To demonstrate this approach, Bacillus pumilus has been developed as an alternative expression platform for the production of alkaline enzymes in reference to the established industrial production host Bacillus licheniformis. To develop the selected B. pumilus as an alternative production host the suggested PSB strategy was applied proceeding in the following steps (dedicated product titers are scaled to the protease titer of Henkel's industrial production strain B. licheniformis at lab scale): Introduction of a protease production plasmid, adaptation of a protease production process (44%), process optimization (92%) and expression optimization (114%). To further evaluate the production capability of the developed B. pumilus platform, the target protease was substituted by an alpha-amylase. The expression performance was tested under the previously optimized protease process conditions and under subsequently adapted process conditions resulting in a maximum product titer of 65% in reference to B. licheniformis protease titer. In this contribution the applied PSB strategy performed very well for the development of B. pumilus as an alternative production strain. Thereby the engineered B. pumilus expression platform even exceeded the protease titer of the industrial production host B. licheniformis by 14%. This result exhibits a remarkable potential of B. pumilus to be the basis for a next generation production host, since the strain has still a large potential for further genetic engineering. The final amylase titer of 65% in reference to B. licheniformis protease titer suggests that the developed B. pumilus expression platform is also suitable for an efficient production of non-proteolytic enzymes reaching a final titer of several grams per liter without complex process modifications.
    Full-text · Article · Mar 2014 · Microbial Cell Factories
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    • "Gram-positive bacteria of the genus Bacillus are widely appreciated as industrial workhorses for the production of various enzymes owing to their ability of secreting proteins into the extracellular medium [1]. Among the huge number of commercial enzymes, proteases are of great importance for their extensive applications in detergent , tanning, food processing, silk degumming, medical diagnosis, bioconversion, waste treatment, and peptide synthesis [2] [3] [4] [5]. Currently, proteases have accounted for up to 60% of the total enzyme sales in the global market, in which alkaline proteases represent the largest portion [6]. "

    Full-text · Dataset · Dec 2013
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    • "Gram-positive bacteria of the genus Bacillus are widely appreciated as industrial workhorses for the production of various enzymes owing to their ability of secreting proteins into the extracellular medium [1]. Among the huge number of commercial enzymes, proteases are of great importance for their extensive applications in detergent , tanning, food processing, silk degumming, medical diagnosis, bioconversion, waste treatment, and peptide synthesis [2] [3] [4] [5]. Currently, proteases have accounted for up to 60% of the total enzyme sales in the global market, in which alkaline proteases represent the largest portion [6]. "

    Full-text · Dataset · Dec 2013
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