Intranasal steroids decrease eosinophils but not mucin expression

Cardiovascular Research Institute, Box 0130, University of California San Francisco, San Francisco, CA 94 143-0130, USA.
European Respiratory Journal (Impact Factor: 7.64). 11/2004; 24(4):594-600. DOI: 10.1183/09031936.04.00014404
Source: PubMed


Increased mucin expression is a feature of nasal polyposis. Corticosteroids reduce polyp size and symptoms, but their effect on mucin production remains unknown. In this study, the effects of intranasal corticosteroids on MUC5AC mucin expression, nasal resistance, eosinophil and neutrophil infiltration, epidermal growth factor receptor (EGFR), interleukin (IL)-8, and tumour necrosis factor (TNF)-alpha expression was assessed in nasal polyps. In nine subjects, one nasal polyp was removed surgically before treatment and another was removed after 8 weeks of intranasal fluticasone (400 Tissues were processed for in situ hybridisation and immunohistochemical staining. Described effects of fluticasone on nasal polyps (reduction in nasal resistance and in eosinophil infiltration) were evaluated. Morphometric analysis was performed to assess the effect of fluticasone on epithelial-, MUC5AC-, EGFR- and IL-8-stained areas, TNF-alpha-stained cells, and neutrophil numbers. Treatment with fluticasone decreased nasal resistance and intra-epithelial eosinophils. The MUC5AC-stained area in the epithelium was unchanged by treatment; MUC5AC mRNA expression was unaffected by treatment. EGFR-stained area, intra-epithelial neutrophil numbers, IL-8 and TNF-alpha expression were also unchanged by therapy. Intranasal fluticasone was effective in decreasing nasal airflow resistance and intra-epithelial eosinophils but had no effect on mucin or epidermal growth factor receptor expression or on neutrophil recruitment.

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    • "The same study also demonstrated that apigenin-mediated MUC1 downregulation was associated with increased apoptosis and decreased clonogenic potential. EGF through EGFR signaling stimulates MUC5AC expression in a human airway epithelial cell line [98] and its hypersecretion is associated with severe pulmonary disorders like asthma, chronic bronchitis, cystic fibrosis and bronchiectasis [99]. Apigenin treatment was shown to decrease both de novo [100] as well as EGF-induced MUC5AC expression [100] in lung cancer NCI-H292 cells. "
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    • "The advent of topically administered corticosteroids has improved the treatment of upper (NP and rhinitis) and lower (asthma, chronic obstructive airways disease) airways disease (Fokkens et al 2007). Their clinical efficacy is achieved by a combination of anti inflammatory effects along with their ability to reduce airway eosinophillic infiltration by preventing their increased viability and activation (Burgel et al 2004). Both topical and systemic glucocorticoids may affect the eosinophil function by both directly reducing eosinophil viability and function or indirectly reducing the secretion of chemotactic cytokines by nasal mucosa and polyp epithelial cells (Roca-Ferrer et al 1997). "
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