Evaluation of Hyaluronan from Different Sources: Streptococcus z ooepidemicus , Rooster Comb, Bovine Vitreous, and Human Umbilical Cord

Department of Surgery, University of Washington Seattle, Seattle, Washington, United States
Biomacromolecules (Impact Factor: 5.75). 10/2004; 5(6):2122-7. DOI: 10.1021/bm0498427
Source: PubMed


Sodium hyaluronate (HA) is widely distributed in extracellular matrixes and can play a role in orchestrating cell function. Consequently, many investigators have looked at the effect of exogenous HA on cell behavior in vitro. HA can be isolated from several sources (e.g., bacterial, rooster comb, umbilical cord) and therefore can possess diverse impurities. This current study compares the measured impurities and the differences in biological activity between HA preparations from these sources. It was demonstrated that nucleic acid and protein content was highest in human umbilical cord and bovine vitreous HA and was low in bacterial and rooster comb HA. Macrophages exposed to human umbilical cord HA produced significantly higher amounts of TNF-alpha relative to control or bacterial-derived HA. These results indicate that the source of HA should be considered due to differences in the amounts and types of contaminants that could lead to widely different behaviors in vitro and in vivo.

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Available from: Sam Arbabi, Jun 03, 2014
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    • "For biomedical applications, at first, HA was extracted and purified from rooster combs. Nowadays, HA production by microbial culture is preferred since it allows the standardization of molar mass distribution (Huang, Chen, & Chen, 2008; Kim, Lee, & Moon, 2006; Shiedlin et al., 2004; Yamada & Kawasaki, 2005). After the microbial culture step, HA has to be separated from cells and purified from the various molecules composing the complex culture medium. "
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    ABSTRACT: The cetyltrimethylammonium bromide turbidimetric method (CTM) has been developed to quantify the hyaluronic acid (HA) in complex media to overcome the lack of selectivity and specificity of the standard carbazole method. The objective of this work is to assess the potential application of CTM to determine HA concentration. Factors such as duration of incubation, linearity range, HA size and form (natural linear HA or cross linked HA), pH and ionic environment impact were investigated. The incubation time was set to 10 min and the calibration curve was linear up to 0.6 g L−1. The quantitative method was relevant whatever the HA size and form, and also for a wide range of conditions. The robustness of the CTM added to its high specificity and simplicity demonstrated that the CTM is a valuable method that would be an interesting substitute to the carbazole assay for HA quantification.
    Full-text · Article · Nov 2014 · Carbohydrate Polymers
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    • "Originally, it has been obtained and commercialized from diverse mammalian substrates as rooster combs, synovial fluid, VH and umbilical cords [53]. Marine wastes have been also explored in the search of new sources of HA, being only found in VH of various fish species and in cartilage of chondrichthyes [56]. "
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    ABSTRACT: In the last decade, an increasing number of glycosaminoglycans (GAGs), chitin and chitosan applications have been reported. Their commercial demands have been extended to different markets, such as cosmetics, medicine, biotechnology, food and textiles. Marine wastes from fisheries and aquaculture are susceptible sources for polymers but optimized processes for their recovery and production must be developed to satisfy such necessities. In the present work, we have reviewed different alternatives reported in the literature to produce and purify chondroitin sulfate (CS), hyaluronic acid (HA) and chitin/chitosan (CH/CHs) with the aim of proposing environmentally friendly processes by combination of various microbial, chemical, enzymatic and membranes strategies and technologies.
    Full-text · Article · Mar 2013 · Marine Drugs
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    • "A comprehensive overview of the sources from which HA can be isolated has been documented (Shiedlin et al. 2004). It has been reported that preparation of HA from bacterial source contains lower amounts of contaminating protein, endotoxin and nucleotides than those of animal source (Shiedlin et al. 2004). To date, only a few bacterial species are known to produce HA: group A and group C streptococci (Gram positive ) and Pasteurella multocida (Gram negative). "
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    ABSTRACT: Probiotics have been extensively reviewed for decades, emphasizing on improving general gut health. Recently, more studies showed that probiotics may exert other health promoting effects beyond gut well-being, attributed to the rise of the gut-brain-axis correlations. Some of these new benefits include skin health such as improving atopic eczema, atopic dermatitis, healing of burn and scars, skin rejuvenating properties, and improving skin innate immunity. Increasing evidences have also showed that bacterial compounds such as cell wall fragments, their metabolites and dead bacteria can elicit certain immune responses on the skin and improve skin barrier functions. This review aimed to underline the mechanisms or the exact compounds underlying the benefits of bacterial extract on the skin based on evidences from in vivo and in vitro studies. This review could be of help in screening of probiotic strains with potential dermal enhancing properties for topical applications. © 2013 The Authors Journal of Applied Microbiology © 2013 The Society for Applied Microbiology.
    Full-text · Article · Jan 2013 · Journal of Applied Microbiology
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