A neurogenomics approach to gene expression analysis in the developing brain

Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, United States.
Molecular Brain Research (Impact Factor: 2). 01/2005; 132(2):116-27. DOI: 10.1016/j.molbrainres.2004.10.002
Source: PubMed


Secreted and transmembrane proteins provide critical functions in the signaling networks essential for neurogenesis. We used a genetic signal sequence gene trap approach to isolate 189 genes expressed during development in e16.5 whole head, e16.5 hippocampus and e14.5 cerebellum. Gene ontology programs were used to classify the genes into respective biological processes. Four major classes of biological processes known to be important during development were identified: cell communication, cell physiology processes, metabolism and morphogenesis. We used in situ hybridization to determine the temporal and spatial patterns of gene expression in the developing brain using this set of probes. The results demonstrate that gene expression patterns can highlight potential gene functions in specific brain regions. We propose that combining bioinformatics with the gene expression pattern is an effective strategy to identify genes that may play critical roles during brain development.

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    • "In this regard, it is particularly useful to compare the embryonic expression patterns with those of the adult as some genes may exhibit distinct functions in the developing and adult brain. BGEM BGEM is a growing atlas of genes expressed in the developing and adult mouse brain (Jensen et al. 2004). BGEM is part of the GENSAT project (http://www. "
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